AB134068

Anti-PDGFR alpha (phospho Y720) antibody [EP2478]

20ul selling size
RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal PDGFR alpha phospho Y720 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Mouse samples. Cited in 6 publications.

View Alternative Names

CD140a, PDGFR2, RHEPDGFRA, PDGFRA, Platelet-derived growth factor receptor alpha, PDGF-R-alpha, PDGFR-alpha, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a antigen, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, PDGFR-2

3 Images

Supplier Data

Immunocytochemistry - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (AB134068)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling PDGFR alpha with ab134068 at 1/100 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of NIH/3T3 cells. ab195889 AlexaFluor® 594-conjugated Anti-alpha Tubulin was used to counterstain tubulin at 1/200 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (AB134068)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (AB134068)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PDGFR alpha with ab134068 at 1/1000 dilution (Red) compared with an isotype control (Black) and an unlabelled control (Cell without incubation with primary antibody and secondary antibody (Blue)). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) was used as the secondary antibody.

Supplier Data

Western blot - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (AB134068)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (ab134068) at 1/10000 dilution

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Pervanadate for 30 min whole cell lysate at 15 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Pervanadate for 30 min whole cell lysate, then membrane treated with Alkaline phosphatase at 15 µg

Secondary

All lanes:

Western blot

Observed band size: 190 kDa

false

Exposure time: 40s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2478

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDGFR alpha also known as platelet-derived growth factor receptor alpha is a cell surface receptor tyrosine kinase with a mass of approximately 123 kDa. It plays a mechanical role in transmitting signals across the cell membrane initiating cellular responses. PDGFR alpha is widely expressed in mesenchymal cells including fibroblasts and smooth muscle cells. Researchers frequently employ techniques such as PDGFRA IHC and PDGFRA staining to study the localization and expression levels in tissues.

Biological function summary

PDGFR alpha mediates cell proliferation survival and differentiation especially in embryonic development and wound healing. The receptor often forms a complex by dimerizing with itself or with PDGFR beta to activate intracellular signaling cascades. This process is initiated upon ligand binding which triggers autophosphorylation on tyrosine residues. The receptor is also recognized in the detection of PDGFRA with assays such as PDGFRA ELISA.

Pathways

PDGFR alpha significantly affects the MAPK and PI3K/AKT signaling pathways which regulate cellular growth and survival signals. Through these pathways PDGFR alpha interacts with related proteins like SHP-2 and PI3K contributing to signal transduction involved in cellular responses. This role links PDGFR alpha to important cellular processes via its kinase activity and signal integration.

PDGFR alpha is implicated in conditions such as gastrointestinal stromal tumors (GISTs) and idiopathic pulmonary fibrosis (IPF). It connects to proteins such as KIT in GISTs where mutations cause aberrant receptor signaling leading to tumorigenesis. Aberrant PDGFR alpha signaling also associates with IPF a disorder characterized by increased fibroblast proliferation and fibrotic tissue formation. These connections make PDGFR alpha a significant target for research and therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.

See full target information PDGFRA phospho Y720

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell communication and signaling 19:e70025 PubMed40520075

2025

Adipose-derived stem cell exosomes alleviate TGF-β1-induced urethral stricture fibrosis by suppressing the TGF-β/Smad pathway and downstream PDGFR-β/RAS/ERK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Liang,Chao Deng,Hang Guo,Zhenghao Dai,Yiwen Jiang,Yuting Lu,Weiguo Chen

Nature communications 16:471 PubMed39773984

2025

Endothelial-secreted Endocan activates PDGFRA and regulates vascularity and spatial phenotype in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Soniya Bastola,Marat S Pavlyukov,Neel Sharma,Yasmin Ghochani,Mayu A Nakano,Sree Deepthi Muthukrishnan,Sang Yul Yu,Min Soo Kim,Alireza Sohrabi,Natalia P Biscola,Daisuke Yamashita,Ksenia S Anufrieva,Tatyana F Kovalenko,Grace Jung,Tomas Ganz,Beatrice O'Brien,Riki Kawaguchi,Yue Qin,Stephanie K Seidlits,Alma L Burlingame,Juan A Oses-Prieto,Leif A Havton,Steven A Goldman,Anita B Hjelmeland,Ichiro Nakano,Harley I Kornblum

Nature communications 14:4467 PubMed37491377

2023

Circular RNA encoded MET variant promotes glioblastoma tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Zhong,Xujia Wu,Yixin Gao,Junju Chen,Maolei Zhang,Huangkai Zhou,Jia Yang,Feizhe Xiao,Xuesong Yang,Nunu Huang,Haoyue Qi,Xiuxing Wang,Fan Bai,Yu Shi,Nu Zhang

PloS one 14:e0214654 PubMed30925179

2019

Characterization of pulmonary intimal sarcoma cells isolated from a surgical specimen: In vitro and in vivo study.

Applications

Unspecified application

Species

Unspecified reactive species

Takayuki Jujo Sanada,Seiichiro Sakao,Akira Naito,Hatsue Ishibashi-Ueda,Masaki Suga,Hiroki Shoji,Hideki Miwa,Rika Suda,Shunichiro Iwasawa,Yuji Tada,Keiichi Ishida,Nobuhiro Tanabe,Koichiro Tatsumi

Clinical cancer research : an official journal of 25:4179-4193 PubMed30867219

2019

Unexpected Activities in Regulating Ciliation Contribute to Off-target Effects of Targeted Drugs.

Applications

Unspecified application

Species

Unspecified reactive species

Anna A Kiseleva,Vladislav A Korobeynikov,Anna S Nikonova,Peishan Zhang,Petr Makhov,Alexander Y Deneka,Margret B Einarson,Ilya G Serebriiskii,Hanqing Liu,Jeffrey R Peterson,Erica A Golemis

The Journal of clinical investigation 129:1596-1611 PubMed30730307

2019

Targeting compensatory MEK/ERK activation increases JAK inhibitor efficacy in myeloproliferative neoplasms.

Applications

Unspecified application

Species

Unspecified reactive species

Simona Stivala,Tamara Codilupi,Sime Brkic,Anne Baerenwaldt,Nilabh Ghosh,Hui Hao-Shen,Stephan Dirnhofer,Matthias S Dettmer,Cedric Simillion,Beat A Kaufmann,Sophia Chiu,Matthew Keller,Maria Kleppe,Morgane Hilpert,Andreas S Buser,Jakob R Passweg,Thomas Radimerski,Radek C Skoda,Ross L Levine,Sara C Meyer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDGFR alpha (phospho Y720) antibody [EP2478]

Immunocytochemistry - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (AB134068)

Flow Cytometry (Intracellular) - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (AB134068)

Western blot - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (AB134068)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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