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Rabbit Recombinant Monoclonal PDGFR alpha phospho Y720 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Mouse samples. Cited in 4 publications.

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Images

Immunocytochemistry - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (AB134068), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (AB134068), expandable thumbnail
  • Western blot - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (AB134068), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Predicted
Not recommended
Predicted
Not recommended
Predicted
Mouse
Tested
Not recommended
Tested
Not recommended
Tested
Rat
Predicted
Not recommended
Predicted
Not recommended
Predicted

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Predicted
Predicted

Species
Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/10000
Notes

-

Predicted
Predicted

Species
Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.

Alternative names

CD140a, PDGFR2, RHEPDGFRA, PDGFRA, Platelet-derived growth factor receptor alpha, PDGF-R-alpha, PDGFR-alpha, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a antigen, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, PDGFR-2

Recommended products

Rabbit Recombinant Monoclonal PDGFR alpha phospho Y720 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Mouse samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP2478
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PDGFR alpha also known as platelet-derived growth factor receptor alpha is a cell surface receptor tyrosine kinase with a mass of approximately 123 kDa. It plays a mechanical role in transmitting signals across the cell membrane initiating cellular responses. PDGFR alpha is widely expressed in mesenchymal cells including fibroblasts and smooth muscle cells. Researchers frequently employ techniques such as PDGFRA IHC and PDGFRA staining to study the localization and expression levels in tissues.

Biological function summary

PDGFR alpha mediates cell proliferation survival and differentiation especially in embryonic development and wound healing. The receptor often forms a complex by dimerizing with itself or with PDGFR beta to activate intracellular signaling cascades. This process is initiated upon ligand binding which triggers autophosphorylation on tyrosine residues. The receptor is also recognized in the detection of PDGFRA with assays such as PDGFRA ELISA.

Pathways

PDGFR alpha significantly affects the MAPK and PI3K/AKT signaling pathways which regulate cellular growth and survival signals. Through these pathways PDGFR alpha interacts with related proteins like SHP-2 and PI3K contributing to signal transduction involved in cellular responses. This role links PDGFR alpha to important cellular processes via its kinase activity and signal integration.

Associated diseases and disorders

PDGFR alpha is implicated in conditions such as gastrointestinal stromal tumors (GISTs) and idiopathic pulmonary fibrosis (IPF). It connects to proteins such as KIT in GISTs where mutations cause aberrant receptor signaling leading to tumorigenesis. Aberrant PDGFR alpha signaling also associates with IPF a disorder characterized by increased fibroblast proliferation and fibrotic tissue formation. These connections make PDGFR alpha a significant target for research and therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (ab134068), expandable thumbnail

    Immunocytochemistry - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (ab134068)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling PDGFR alpha with ab134068 at 1/100 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of NIH/3T3 cells. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 AlexaFluor® 594-conjugated Anti-alpha Tubulin was used to counterstain tubulin at 1/200 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (ab134068), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (ab134068)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PDGFR alpha with ab134068 at 1/1000 dilution (Red) compared with an isotype control (Black) and an unlabelled control (Cell without incubation with primary antibody and secondary antibody (Blue)). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) was used as the secondary antibody.

  • Western blot - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (ab134068), expandable thumbnail

    Western blot - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (ab134068)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PDGFR alpha (phospho Y720) antibody [EP2478] (ab134068) at 1/10000 dilution

    Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Pervanadate for 30 min whole cell lysate at 15 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Pervanadate for 30 min whole cell lysate, then membrane treated with Alkaline phosphatase at 15 µg

    Secondary

    All lanes: Western blot

    Observed band size: 190 kDa

    Exposure time: 40s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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