AB314096

Anti-PDGFR beta antibody [BLR081G] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal PDGFR beta antibody. Suitable for Flow Cyt, ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PDGFRB aa 1-550.

View Alternative Names

CD140b, PDGFR, PDGFR1, PDGFRB, Platelet-derived growth factor receptor beta, PDGF-R-beta, PDGFR-beta, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1

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Supplier Data

Immunocytochemistry - Anti-PDGFR beta antibody [BLR081G] - BSA free (AB314096)

Detection of human PDGFR beta in FFPE A-172 cells by ICC. Antibody : Rabbit anti-PDGFR beta recombinant monoclonal antibody. Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Supplier Data

Immunocytochemistry - Anti-PDGFR beta antibody [BLR081G] - BSA free (AB314096)

Detection of human PDGFR beta in FFPE OVCAR-8 cells by ICC. Antibody : Rabbit anti-PDGFR beta recombinant monoclonal antibody. Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Flow Cytometry - Anti-PDGFR beta antibody [BLR081G] - BSA free (AB314096)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-PDGFR beta antibody [BLR081G] - BSA free (AB314096)

Detection of human PDGFR beta (shaded) in OVCAR8 cells by flow cytometry. Antibody : Rabbit anti-PDGFR beta recombinant monoclonal antibody [BLR081G] (A700-081) or isotype control (unshaded). Secondary : DyLight® 650-conjugated goat anti-rabbit IgG.

Supplier Data

Immunoprecipitation - Anti-PDGFR beta antibody [BLR081G] - BSA free (AB314096)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from BJ cells prepared using NETN lysis buffer. Antibodies : Rabbit anti-PDGFR beta recombinant monoclonal antibody [BLR081G] (A700-081) used for IP at 6 µl per reaction. PDGFR beta was also immunoprecipitated by rabbit anti-PDGFR beta recombinant monoclonal antibody [BL-1184-1E8]. For blotting immunoprecipitated PDGFR beta, A700-081 was used at 1 : 1000. Chemiluminescence with an exposure time of 10 seconds.

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Supplier Data

Western blot - Anti-PDGFR beta antibody [BLR081G] - BSA free (AB314096)

Samples : Whole cell lysate (50 µg) from BJ, HEK293T, GaMG, A-549, U2OS, MCF-7, IMR-90, and Jurkat cells prepared using NETN lysis buffer. Antibody : Rabbit anti-PDGFR beta recombinant monoclonal antibody [BLR081G] (A700-081 lot 1) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 minutes. Lower Panel : Rabbit anti-eEF2 (A301-688A).

All lanes:

Western blot - Anti-PDGFR beta antibody [BLR081G] - BSA free (ab314096) at 1/1000 dilution

Lane 1:

BJ Whole cell lysate at 50 µg

Lane 2:

HEK293T Whole cell lysate at 50 µg

Lane 3:

GaMG Whole cell lysate at 50 µg

Lane 4:

A-549 Whole cell lysate at 50 µg

Lane 5:

U2OS Whole cell lysate at 50 µg

Lane 6:

MCF-7 Whole cell lysate at 50 µg

Lane 7:

IMR-90 Whole cell lysate at 50 µg

Lane 8:

Jurkat Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG.

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR081G

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, ICC, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human PDGFRB aa 1-550. The exact immunogen used to generate this antibody is proprietary information.

P09619

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDGFR beta also known as PDGFR-b or PDGFRB is a cell surface tyrosine kinase receptor with a molecular mass of about 180 kDa. It binds the PDGF (platelet-derived growth factor) family of ligands. PDGFR beta is commonly found in various tissues including blood vessels and connective tissues and is highly expressed in cells like pericytes and fibroblasts. The receptor plays a critical role in cell signaling mechanisms involving proliferation chemotaxis and survival.

Biological function summary

The receptor plays an essential role in the regulation of cell growth and development. PDGFR beta undergoes dimerization and autophosphorylation upon ligand binding initiating a series of downstream signaling cascades. This receptor is often part of a complex with other receptor proteins promoting interactions necessary for signal propagation. Its main biological functions include mediating cellular responses to environmental signals that contribute to tissue repair and angiogenesis.

Pathways

PDGFR beta is an important player within the PI3K-Akt and MAPK signaling pathways. It works alongside proteins such as PI3K and Ras to regulate cellular responses related to growth and survival. These pathways facilitate cross-talk with other cellular processes influencing various cellular outcomes. This receptor's activity regulates critical physiological functions by providing signals that maintain cellular homeostasis under various physiological conditions.

PDGFR beta has significant implications in the progression of cancer and fibrotic diseases. Its overexpression or mutation can lead to anomalous signaling that contributes to tumorigenesis particularly in connective tissue tumors known as sarcomas. Additionally PDGFR beta's role in promoting fibroblast activity makes it relevant in fibrotic diseases such as pulmonary fibrosis. Abnormal activation of PDGFR beta can interact with related proteins like VEGF receptors enhancing pathogenic responses and contributing to disease severity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the PDGFRB gene is a tyrosine-protein kinase that functions as a cell-surface receptor for various PDGF homodimers and heterodimers, playing an essential role in embryonic development, cell proliferation, survival, differentiation, chemotaxis, and migration. It is crucial for blood vessel development by promoting the proliferation and migration of, as well as recruitment of, pericytes and smooth muscle cells to endothelial cells. It has a role in vascular smooth muscle cell migration and neointima formation at injury sites, and is necessary for normal cardiovascular development and pericyte recruitment in kidney glomeruli. The protein facilitates actin cytoskeleton rearrangement and membrane ruffle formation. Ligand binding activates signaling cascades depending on the ligand bound and interactions with PDGFRA, leading to phosphorylation of several substrates, activation of signaling pathways such as AKT1, MAPK, and SRC family kinases, and production of molecules like diacylglycerol and inositol trisphosphate. Receptor signaling is downregulated by protein phosphatases and receptor internalization. This supplementary information is collated from multiple sources and compiled automatically.

See full target information PDGFRB

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDGFR beta antibody [BLR081G] - BSA free

Immunocytochemistry - Anti-PDGFR beta antibody [BLR081G] - BSA free (AB314096)

Immunocytochemistry - Anti-PDGFR beta antibody [BLR081G] - BSA free (AB314096)

Flow Cytometry - Anti-PDGFR beta antibody [BLR081G] - BSA free (AB314096)

Immunoprecipitation - Anti-PDGFR beta antibody [BLR081G] - BSA free (AB314096)

Western blot - Anti-PDGFR beta antibody [BLR081G] - BSA free (AB314096)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise