Skip to main content

Rabbit Recombinant Monoclonal PDGFR beta antibody. Suitable for Flow Cyt, ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PDGFRB aa 1-550.

Be the first to review this product! Submit a review

Images

Western blot - Anti-PDGFR beta antibody [BLR081G] - BSA free (AB314096), expandable thumbnail
  • Immunoprecipitation - Anti-PDGFR beta antibody [BLR081G] - BSA free (AB314096), expandable thumbnail
  • Flow Cytometry - Anti-PDGFR beta antibody [BLR081G] - BSA free (AB314096), expandable thumbnail
  • Immunocytochemistry - Anti-PDGFR beta antibody [BLR081G] - BSA free (AB314096), expandable thumbnail
  • Immunocytochemistry - Anti-PDGFR beta antibody [BLR081G] - BSA free (AB314096), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human PDGFRB aa 1-550. The exact immunogen used to generate this antibody is proprietary information. Database link P09619

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICCIPWB
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

The protein expressed by the PDGFRB gene is a tyrosine-protein kinase that functions as a cell-surface receptor for various PDGF homodimers and heterodimers, playing an essential role in embryonic development, cell proliferation, survival, differentiation, chemotaxis, and migration. It is crucial for blood vessel development by promoting the proliferation and migration of, as well as recruitment of, pericytes and smooth muscle cells to endothelial cells. It has a role in vascular smooth muscle cell migration and neointima formation at injury sites, and is necessary for normal cardiovascular development and pericyte recruitment in kidney glomeruli. The protein facilitates actin cytoskeleton rearrangement and membrane ruffle formation. Ligand binding activates signaling cascades depending on the ligand bound and interactions with PDGFRA, leading to phosphorylation of several substrates, activation of signaling pathways such as AKT1, MAPK, and SRC family kinases, and production of molecules like diacylglycerol and inositol trisphosphate. Receptor signaling is downregulated by protein phosphatases and receptor internalization. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

CD140b, PDGFR, PDGFR1, PDGFRB, Platelet-derived growth factor receptor beta, PDGF-R-beta, PDGFR-beta, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1

Recommended products

Rabbit Recombinant Monoclonal PDGFR beta antibody. Suitable for Flow Cyt, ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PDGFRB aa 1-550.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human PDGFRB aa 1-550. The exact immunogen used to generate this antibody is proprietary information. Database link P09619
Clone number
BLR081G
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PDGFR beta also known as PDGFR-b or PDGFRB is a cell surface tyrosine kinase receptor with a molecular mass of about 180 kDa. It binds the PDGF (platelet-derived growth factor) family of ligands. PDGFR beta is commonly found in various tissues including blood vessels and connective tissues and is highly expressed in cells like pericytes and fibroblasts. The receptor plays a critical role in cell signaling mechanisms involving proliferation chemotaxis and survival.

Biological function summary

The receptor plays an essential role in the regulation of cell growth and development. PDGFR beta undergoes dimerization and autophosphorylation upon ligand binding initiating a series of downstream signaling cascades. This receptor is often part of a complex with other receptor proteins promoting interactions necessary for signal propagation. Its main biological functions include mediating cellular responses to environmental signals that contribute to tissue repair and angiogenesis.

Pathways

PDGFR beta is an important player within the PI3K-Akt and MAPK signaling pathways. It works alongside proteins such as PI3K and Ras to regulate cellular responses related to growth and survival. These pathways facilitate cross-talk with other cellular processes influencing various cellular outcomes. This receptor's activity regulates critical physiological functions by providing signals that maintain cellular homeostasis under various physiological conditions.

Associated diseases and disorders

PDGFR beta has significant implications in the progression of cancer and fibrotic diseases. Its overexpression or mutation can lead to anomalous signaling that contributes to tumorigenesis particularly in connective tissue tumors known as sarcomas. Additionally PDGFR beta's role in promoting fibroblast activity makes it relevant in fibrotic diseases such as pulmonary fibrosis. Abnormal activation of PDGFR beta can interact with related proteins like VEGF receptors enhancing pathogenic responses and contributing to disease severity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-PDGFR beta antibody [BLR081G] - BSA free (ab314096), expandable thumbnail

    Western blot - Anti-PDGFR beta antibody [BLR081G] - BSA free (ab314096)

    Samples: Whole cell lysate (50 µg) from BJ, HEK293T, GaMG, A-549, U2OS, MCF-7, IMR-90, and Jurkat cells prepared using NETN lysis buffer. Antibody:Rabbit anti-PDGFR beta recombinant monoclonal antibody [BLR081G] (A700-081 lot 1) used at 1:1000. Secondary:HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-eEF2 (A301-688A).

    All lanes: Western blot - Anti-PDGFR beta antibody [BLR081G] - BSA free (ab314096) at 1/1000 dilution

    Lane 1: BJ Whole cell lysate at 50 µg

    Lane 2: HEK293T Whole cell lysate at 50 µg

    Lane 3: GaMG Whole cell lysate at 50 µg

    Lane 4: A-549 Whole cell lysate at 50 µg

    Lane 5: U2OS Whole cell lysate at 50 µg

    Lane 6: MCF-7 Whole cell lysate at 50 µg

    Lane 7: IMR-90 Whole cell lysate at 50 µg

    Lane 8: Jurkat Whole cell lysate at 50 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG.

  • Immunoprecipitation - Anti-PDGFR beta antibody [BLR081G] - BSA free (ab314096), expandable thumbnail

    Immunoprecipitation - Anti-PDGFR beta antibody [BLR081G] - BSA free (ab314096)

    Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from BJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PDGFR beta recombinant monoclonal antibody [BLR081G] (A700-081) used for IP at 6 µl per reaction. PDGFR beta was also immunoprecipitated by rabbit anti-PDGFR beta recombinant monoclonal antibody [BL-1184-1E8]. For blotting immunoprecipitated PDGFR beta, A700-081 was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.

  • Flow Cytometry - Anti-PDGFR beta antibody [BLR081G] - BSA free (ab314096), expandable thumbnail

    Flow Cytometry - Anti-PDGFR beta antibody [BLR081G] - BSA free (ab314096)

    Detection of human PDGFR beta (shaded) in OVCAR8 cells by flow cytometry. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody [BLR081G] (A700-081) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

  • Immunocytochemistry - Anti-PDGFR beta antibody [BLR081G] - BSA free (ab314096), expandable thumbnail

    Immunocytochemistry - Anti-PDGFR beta antibody [BLR081G] - BSA free (ab314096)

    Detection of human PDGFR beta in FFPE OVCAR-8 cells by ICC. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

  • Immunocytochemistry - Anti-PDGFR beta antibody [BLR081G] - BSA free (ab314096), expandable thumbnail

    Immunocytochemistry - Anti-PDGFR beta antibody [BLR081G] - BSA free (ab314096)

    Detection of human PDGFR beta in FFPE A-172 cells by ICC. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com