Anti-PDGFR beta antibody [EPR26830-84]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [EPR26830-84] (AB313777)

Immunohistochemical analysis of paraffin-embedded Human colorectal car tissue labeling PDGFR beta with ab313777 at 1/100 (4.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on stromal cells of human colorectal carcinoma (PMID : 23441134).The section was incubated with ab313777 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [EPR26830-84] (AB313777)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PDGFR beta with ab313777 at 1/100 (4.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human kidney (PMID : 15067514).The section was incubated with ab313777 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [EPR26830-84] (AB313777)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling PDGFR beta with ab313777 at 1/100 (4.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum endothelium (PMID : 9211853).The section was incubated with ab313777 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [EPR26830-84] (AB313777)

Immunohistochemical analysis of paraffin-embedded (A) Wild-type SH-SY5 tissue labeling PDGFR beta with ab313777 at 1/100 (4.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) Wild-type SH-SY5Y cell pellet, no staining on (B) PDGFRB knockout SH-SY5Y cell pellet. The section was incubated with ab313777 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

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Western blot - Anti-PDGFR beta antibody [EPR26830-84] (AB313777)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PDGFR beta antibody [EPR26830-84] (ab313777) at 1/1000 dilution

All lanes:

Human kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 200 kDa,150 kDa

false

Exposure time: 114s

• WB

Supplier Data

Western blot - Anti-PDGFR beta antibody [EPR26830-84] (AB313777)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 28334876, 19246520, 11346654).

In Western blot, ab313777 was shown to bind specifically to PDGFR beta. Two bands were observed at 200 kDa and 150 kDa in wild-type SH-SY5Y cell lysates ( (lane 1) whereas no signal was observed at this size in PDGFRB knockout cell line (lane 2, knockout cell line ab273749/ knockout cell lysate ab275523).

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-PDGFR beta antibody [EPR26830-84] (ab313777) at 1/1000 dilution

Lane 1:

Wild-type SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Western blot - Human PDGFRB knockout SH-SY5Y cell lysate (<a href='/en-us/products/cell-lysates/human-pdgfrb-knockout-sh-sy5y-cell-lysate-ab275523'>ab275523</a>) at 20 µg

Lane 3:

MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg

Lane 4:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 200 kDa,150 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-PDGFR beta antibody [EPR26830-84] (AB313777)

Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human PDGFR alpha. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Exposure time : 10 seconds

All lanes:

Western blot - Anti-PDGFR beta antibody [EPR26830-84] (ab313777) at 1/1000 dilution

Lane 1:

His-tagged human PDGFR beta recombinant fragment at 20 ng

Lane 2:

His-tagged human PDGFR alpha recombinant fragment at 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 60 kDa

false

Exposure time: 10s