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Knockout Tested Rabbit Recombinant Monoclonal PDGFR beta antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Recombinant fragment - Human samples.

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Images

Western blot - Anti-PDGFR beta antibody [EPR26830-84] - BSA and Azide free (AB313778), expandable thumbnail
  • Western blot - Anti-PDGFR beta antibody [EPR26830-84] - BSA and Azide free (AB313778), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [EPR26830-84] - BSA and Azide free (AB313778), expandable thumbnail
  • Western blot - Anti-PDGFR beta antibody [EPR26830-84] - BSA and Azide free (AB313778), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [EPR26830-84] - BSA and Azide free (AB313778), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBIPFlow CytICC/IF
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

The protein expressed by the PDGFRB gene is a tyrosine-protein kinase that functions as a cell-surface receptor for various PDGF homodimers and heterodimers, playing an essential role in embryonic development, cell proliferation, survival, differentiation, chemotaxis, and migration. It is crucial for blood vessel development by promoting the proliferation and migration of, as well as recruitment of, pericytes and smooth muscle cells to endothelial cells. It has a role in vascular smooth muscle cell migration and neointima formation at injury sites, and is necessary for normal cardiovascular development and pericyte recruitment in kidney glomeruli. The protein facilitates actin cytoskeleton rearrangement and membrane ruffle formation. Ligand binding activates signaling cascades depending on the ligand bound and interactions with PDGFRA, leading to phosphorylation of several substrates, activation of signaling pathways such as AKT1, MAPK, and SRC family kinases, and production of molecules like diacylglycerol and inositol trisphosphate. Receptor signaling is downregulated by protein phosphatases and receptor internalization. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

CD140b, PDGFR, PDGFR1, PDGFRB, Platelet-derived growth factor receptor beta, PDGF-R-beta, PDGFR-beta, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal PDGFR beta antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Recombinant fragment - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26830-84
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PDGFR beta also known as PDGFR-b or PDGFRB is a cell surface tyrosine kinase receptor with a molecular mass of about 180 kDa. It binds the PDGF (platelet-derived growth factor) family of ligands. PDGFR beta is commonly found in various tissues including blood vessels and connective tissues and is highly expressed in cells like pericytes and fibroblasts. The receptor plays a critical role in cell signaling mechanisms involving proliferation chemotaxis and survival.

Biological function summary

The receptor plays an essential role in the regulation of cell growth and development. PDGFR beta undergoes dimerization and autophosphorylation upon ligand binding initiating a series of downstream signaling cascades. This receptor is often part of a complex with other receptor proteins promoting interactions necessary for signal propagation. Its main biological functions include mediating cellular responses to environmental signals that contribute to tissue repair and angiogenesis.

Pathways

PDGFR beta is an important player within the PI3K-Akt and MAPK signaling pathways. It works alongside proteins such as PI3K and Ras to regulate cellular responses related to growth and survival. These pathways facilitate cross-talk with other cellular processes influencing various cellular outcomes. This receptor's activity regulates critical physiological functions by providing signals that maintain cellular homeostasis under various physiological conditions.

Associated diseases and disorders

PDGFR beta has significant implications in the progression of cancer and fibrotic diseases. Its overexpression or mutation can lead to anomalous signaling that contributes to tumorigenesis particularly in connective tissue tumors known as sarcomas. Additionally PDGFR beta's role in promoting fibroblast activity makes it relevant in fibrotic diseases such as pulmonary fibrosis. Abnormal activation of PDGFR beta can interact with related proteins like VEGF receptors enhancing pathogenic responses and contributing to disease severity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-PDGFR beta antibody [EPR26830-84] - BSA and Azide free (ab313778), expandable thumbnail

    Western blot - Anti-PDGFR beta antibody [EPR26830-84] - BSA and Azide free (ab313778)

    PDGFR beta Western blot staining of Human kidney tissue lysate using rabbit Anti-PDGFR beta antibody

    This data was developed using Anti-PDGFR beta antibody [EPR26830-84] ab313777, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PDGFR beta antibody [EPR26830-84] (Anti-PDGFR beta antibody [EPR26830-84] ab313777) at 1/1000 dilution

    All lanes: Human kidney tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 200 kDa, 150 kDa

    Exposure time: 114s

  • Western blot - Anti-PDGFR beta antibody [EPR26830-84] - BSA and Azide free (ab313778), expandable thumbnail

    Western blot - Anti-PDGFR beta antibody [EPR26830-84] - BSA and Azide free (ab313778)

    This data was developed using Anti-PDGFR beta antibody [EPR26830-84] ab313777, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 28334876, 19246520, 11346654).

    In Western blot, Anti-PDGFR beta antibody [EPR26830-84] ab313777 was shown to bind specifically to PDGFR beta. Two bands were observed at 200 kDa and 150 kDa in wild-type SH-SY5Y cell lysates ( (lane 1) whereas no signal was observed at this size in PDGFRB knockout cell line (lane 2, knockout cell line Human PDGFRB knockout SH-SY5Y cell line ab273749/ knockout cell lysate Human PDGFRB knockout SH-SY5Y cell lysate ab275523).

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PDGFR beta antibody [EPR26830-84] (Anti-PDGFR beta antibody [EPR26830-84] ab313777) at 1/1000 dilution

    Lane 1: Wild-type SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Western blot - Human PDGFRB knockout SH-SY5Y cell lysate (Human PDGFRB knockout SH-SY5Y cell lysate ab275523) at 20 µg

    Lane 3: MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg

    Lane 4: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 200 kDa, 150 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [EPR26830-84] - BSA and Azide free (ab313778), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [EPR26830-84] - BSA and Azide free (ab313778)

    This data was developed using Anti-PDGFR beta antibody [EPR26830-84] ab313777, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling PDGFR beta with Anti-PDGFR beta antibody [EPR26830-84] ab313777 at 1/100 (4.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum endothelium (PMID: 9211853).The section was incubated with Anti-PDGFR beta antibody [EPR26830-84] ab313777 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-PDGFR beta antibody [EPR26830-84] - BSA and Azide free (ab313778), expandable thumbnail

    Western blot - Anti-PDGFR beta antibody [EPR26830-84] - BSA and Azide free (ab313778)

    This data was developed using Anti-PDGFR beta antibody [EPR26830-84] ab313777, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This antibody does not cross-react with human PDGFR alpha.
    In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
    Exposure time: 10 seconds

    All lanes: Western blot - Anti-PDGFR beta antibody [EPR26830-84] (Anti-PDGFR beta antibody [EPR26830-84] ab313777) at 1/1000 dilution

    Lane 1: His-tagged human PDGFR beta recombinant fragment at 20 ng

    Lane 2: His-tagged human PDGFR alpha recombinant fragment at 20 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 60 kDa

    Exposure time: 10s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [EPR26830-84] - BSA and Azide free (ab313778), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [EPR26830-84] - BSA and Azide free (ab313778)

    This data was developed using Anti-PDGFR beta antibody [EPR26830-84] ab313777, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human colorectal car tissue labeling PDGFR beta with Anti-PDGFR beta antibody [EPR26830-84] ab313777 at 1/100 (4.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on stromal cells of human colorectal carcinoma (PMID: 23441134).The section was incubated with Anti-PDGFR beta antibody [EPR26830-84] ab313777 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [EPR26830-84] - BSA and Azide free (ab313778), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [EPR26830-84] - BSA and Azide free (ab313778)

    This data was developed using Anti-PDGFR beta antibody [EPR26830-84] ab313777, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded (A) Wild-type SH-SY5 tissue labeling PDGFR beta with Anti-PDGFR beta antibody [EPR26830-84] ab313777 at 1/100 (4.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) Wild-type SH-SY5Y cell pellet, no staining on (B) PDGFRB knockout SH-SY5Y cell pellet. The section was incubated with Anti-PDGFR beta antibody [EPR26830-84] ab313777 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [EPR26830-84] - BSA and Azide free (ab313778), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [EPR26830-84] - BSA and Azide free (ab313778)

    This data was developed using Anti-PDGFR beta antibody [EPR26830-84] ab313777, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PDGFR beta with Anti-PDGFR beta antibody [EPR26830-84] ab313777 at 1/100 (4.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human kidney (PMID: 15067514).The section was incubated with Anti-PDGFR beta antibody [EPR26830-84] ab313777 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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