AB134048

Anti-PDGFR beta (phospho Y1021) antibody [EPR2476]

Name: Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048) | Abcam
Brand: Abcam Limited
SKU: AB134048
Price: 330 USD
Availability: InStock
Rating: 1 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal PDGFR beta phospho Y1021 antibody. Suitable for Dot, WB, Flow Cyt (Intra) and reacts with Synthetic peptide, Mouse samples. Cited in 4 publications.

View Alternative Names

CD140b, PDGFR, PDGFR1, PDGFRB, Platelet-derived growth factor receptor beta, PDGF-R-beta, PDGFR-beta, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1

4 Images

Flow Cytometry (Intracellular) - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (AB134048)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (AB134048)

Intracellular Intracellular Flow Cytoremtic analysis of permeabilized NIH/3T3 cells, untreated (green) or pervanadate-treated (red) using anti-PDGF Receptor beta phospho RabMab and pervanadate-treated NIH/3T3 cells using the same antibody preincubated with phospho-PDGF Receptor beta peptide (blue) or non-phospho-PDGF Receptor beta peptide (orange)

Unknown

Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (AB134048)

All lanes:

Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048) at 1/50000 dilution

Lane 1:

NIH/3T3 untreated at 10 µg

Lane 2:

NIH/3T3 treated with Pervanadate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 123 kDa

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Lab

Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (AB134048)

Blocking/Diluting Buffer and concentration 5% NFDM/TBST

All lanes:

Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048) at 1/5000 dilution

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 2:

NIH/3T3 treated with 1mM pervanadate for 30 minutes whole cell lysate at 10 µg

Lane 3:

NIH/3T3 treated with 1mM pervanadate for 30 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 123 kDa

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Lab

Dot Blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (AB134048)

Dot blot analysis of PDGFR beta (phospho Y1021) phospho peptide (Lane 1) and PDGFR beta non-phospho peptide (Lane 2) labeling PDGFR beta (phospho Y1021) phospho peptide with ab134048 at a dilution of 1/1000 dilution (0.54ug/ml). A Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20000 dilution.

Blocking buffer : 5% NFDM/TBST. Dilution buffer : 5% NFDM /TBST .

Carrier free

Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2476

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

Dot, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Synthetic peptide": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Species reactivity
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDGFR beta also known as PDGFR-b or PDGFRB is a cell surface tyrosine kinase receptor with a molecular mass of about 180 kDa. It binds the PDGF (platelet-derived growth factor) family of ligands. PDGFR beta is commonly found in various tissues including blood vessels and connective tissues and is highly expressed in cells like pericytes and fibroblasts. The receptor plays a critical role in cell signaling mechanisms involving proliferation chemotaxis and survival.

Biological function summary

The receptor plays an essential role in the regulation of cell growth and development. PDGFR beta undergoes dimerization and autophosphorylation upon ligand binding initiating a series of downstream signaling cascades. This receptor is often part of a complex with other receptor proteins promoting interactions necessary for signal propagation. Its main biological functions include mediating cellular responses to environmental signals that contribute to tissue repair and angiogenesis.

Pathways

PDGFR beta is an important player within the PI3K-Akt and MAPK signaling pathways. It works alongside proteins such as PI3K and Ras to regulate cellular responses related to growth and survival. These pathways facilitate cross-talk with other cellular processes influencing various cellular outcomes. This receptor's activity regulates critical physiological functions by providing signals that maintain cellular homeostasis under various physiological conditions.

PDGFR beta has significant implications in the progression of cancer and fibrotic diseases. Its overexpression or mutation can lead to anomalous signaling that contributes to tumorigenesis particularly in connective tissue tumors known as sarcomas. Additionally PDGFR beta's role in promoting fibroblast activity makes it relevant in fibrotic diseases such as pulmonary fibrosis. Abnormal activation of PDGFR beta can interact with related proteins like VEGF receptors enhancing pathogenic responses and contributing to disease severity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the PDGFRB gene is a tyrosine-protein kinase that functions as a cell-surface receptor for various PDGF homodimers and heterodimers, playing an essential role in embryonic development, cell proliferation, survival, differentiation, chemotaxis, and migration. It is crucial for blood vessel development by promoting the proliferation and migration of, as well as recruitment of, pericytes and smooth muscle cells to endothelial cells. It has a role in vascular smooth muscle cell migration and neointima formation at injury sites, and is necessary for normal cardiovascular development and pericyte recruitment in kidney glomeruli. The protein facilitates actin cytoskeleton rearrangement and membrane ruffle formation. Ligand binding activates signaling cascades depending on the ligand bound and interactions with PDGFRA, leading to phosphorylation of several substrates, activation of signaling pathways such as AKT1, MAPK, and SRC family kinases, and production of molecules like diacylglycerol and inositol trisphosphate. Receptor signaling is downregulated by protein phosphatases and receptor internalization. This supplementary information is collated from multiple sources and compiled automatically.

See full target information PDGFRB phospho Y1021

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell communication and signaling 19:e70048 PubMed41040887

2025

Targeting the PI3K/Akt/NF-κB axis: Cluster of differentiation 5-like-mediated immunometabolic regulation of macrophage polarization in abdominal aortic aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Hemoren Yi,Nan Liu,Zhengyang Wu,Lei Li,Tingting Li,Qixiang Liu,Man Duan,Taihu Wan

International immunopharmacology 115:109702 PubMed37724952

2023

Thymosin β4 preserves vascular smooth muscle phenotype in atherosclerosis via regulation of low density lipoprotein related protein 1 (LRP1).

Applications

Unspecified application

Species

Unspecified reactive species

Sonali Munshaw,Andia N Redpath,Benjamin T Pike,Nicola Smart

Bioscience trends 17:136-147 PubMed36823043

2023

FOXA2 plays a critical role in hepatocellular carcinoma progression and lenvatinib-associated drug resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengxia Wang,Junyi Shen,Chuwen Chen,Tianfu Wen,Chuan Li

Clinical cancer research : an official journal of the American Association for Cancer Research 25:1070-1086 PubMed30397177

2018

Targeting an Autocrine Regulatory Loop in Cancer Stem-like Cells Impairs the Progression and Chemotherapy Resistance of Bladder Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kai-Jian Wang,Chao Wang,Li-He Dai,Jun Yang,Hai Huang,Xiao-Jing Ma,Zhe Zhou,Ze-Yu Yang,Wei-Dong Xu,Mei-Mian Hua,Xin Lu,Shu-Xiong Zeng,Hui-Qing Wang,Zhen-Sheng Zhang,Yan-Qiong Cheng,Dan Liu,Qin-Qin Tian,Ying-Hao Sun,Chuan-Liang Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDGFR beta (phospho Y1021) antibody [EPR2476]

Flow Cytometry (Intracellular) - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (AB134048)

Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (AB134048)

Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (AB134048)

Dot Blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (AB134048)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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