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Rabbit Recombinant Monoclonal PDGFR beta phospho Y1021 antibody. Carrier free. Suitable for Dot, WB, Flow Cyt (Intra) and reacts with Synthetic peptide, Mouse samples. Cited in 1 publication.

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Images

Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free (AB248657), expandable thumbnail
  • Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free (AB248657), expandable thumbnail
  • Dot Blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free (AB248657), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFDotWBIHC-PFlow Cyt (Intra)
Mouse
Not recommended
Expected
Tested
Not recommended
Expected
Synthetic peptide
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Synthetic peptide
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Target data

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.

Alternative names

CD140b, PDGFR, PDGFR1, PDGFRB, Platelet-derived growth factor receptor beta, PDGF-R-beta, PDGFR-beta, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1

Recommended products

Rabbit Recombinant Monoclonal PDGFR beta phospho Y1021 antibody. Carrier free. Suitable for Dot, WB, Flow Cyt (Intra) and reacts with Synthetic peptide, Mouse samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR2476
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248657 is the carrier-free version of Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] ab134048.

Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PDGFR beta also known as PDGFR-b or PDGFRB is a cell surface tyrosine kinase receptor with a molecular mass of about 180 kDa. It binds the PDGF (platelet-derived growth factor) family of ligands. PDGFR beta is commonly found in various tissues including blood vessels and connective tissues and is highly expressed in cells like pericytes and fibroblasts. The receptor plays a critical role in cell signaling mechanisms involving proliferation chemotaxis and survival.

Biological function summary

The receptor plays an essential role in the regulation of cell growth and development. PDGFR beta undergoes dimerization and autophosphorylation upon ligand binding initiating a series of downstream signaling cascades. This receptor is often part of a complex with other receptor proteins promoting interactions necessary for signal propagation. Its main biological functions include mediating cellular responses to environmental signals that contribute to tissue repair and angiogenesis.

Pathways

PDGFR beta is an important player within the PI3K-Akt and MAPK signaling pathways. It works alongside proteins such as PI3K and Ras to regulate cellular responses related to growth and survival. These pathways facilitate cross-talk with other cellular processes influencing various cellular outcomes. This receptor's activity regulates critical physiological functions by providing signals that maintain cellular homeostasis under various physiological conditions.

Associated diseases and disorders

PDGFR beta has significant implications in the progression of cancer and fibrotic diseases. Its overexpression or mutation can lead to anomalous signaling that contributes to tumorigenesis particularly in connective tissue tumors known as sarcomas. Additionally PDGFR beta's role in promoting fibroblast activity makes it relevant in fibrotic diseases such as pulmonary fibrosis. Abnormal activation of PDGFR beta can interact with related proteins like VEGF receptors enhancing pathogenic responses and contributing to disease severity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free (ab248657), expandable thumbnail

    Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free (ab248657)

    This data was developed using Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] ab134048, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] ab134048) at 1/50000 dilution

    Lane 1: NIH/3T3 untreated at 10 µg

    Lane 2: NIH/3T3 treated with Pervanadate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 123 kDa

  • Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free (ab248657), expandable thumbnail

    Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free (ab248657)

    This data was developed using Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] ab134048, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] ab134048) at 1/5000 dilution

    Lane 1: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Lane 2: NIH/3T3 treated with 1mM pervanadate for 30 minutes whole cell lysate at 10 µg

    Lane 3: NIH/3T3 treated with 1mM pervanadate for 30 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 123 kDa

  • Dot Blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free (ab248657), expandable thumbnail

    Dot Blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free (ab248657)

    This data was developed using Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] ab134048, the same antibody clone in a different buffer formulation.Dot blot analysis of PDGFR beta (phospho Y1021) phospho peptide (Lane 1) and PDGFR beta non-phospho peptide (Lane 2) labeling PDGFR beta (phospho Y1021) phospho peptide with Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] ab134048 at a dilution of 1/1000 dilution (0.54ug/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST .

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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