Rabbit Recombinant Monoclonal PDGFR beta phospho Y751 antibody. Suitable for Dot, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.
Images
![Western blot - Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (AB218534), expandable thumbnail](https://content.abcam.com/products/images/pdgfr-beta-phospho-y751-antibody-epr21744-ab218534--western-blot-img170563.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (AB218534)")
![Dot Blot - Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (AB218534), expandable thumbnail](https://content.abcam.com/products/images/pdgfr-beta-phospho-y751-antibody-epr21744-ab218534--dot-blot-img12632.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (AB218534)")
Publications
Filter by
- Medical oncology (Northwood, London, England) 40:2402023PDGF-BB/PDGFRβ induces tumour angiogenesis via enhancing PKM2 mediated by the PI3K/AKT pathway in Wilms' tumour.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo-Tao Sang,Chang-Dong Wang,Xing Liu,Jia-Qi Guo,Jia-Yi Lai,Xiang-Mei WuPubMed 37442847
- Cell death & disease 13:3512022EGFR/MET promotes hepatocellular carcinoma metastasis by stabilizing tumor cells and resisting to RTKs inhibitors in circulating tumor microemboli.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShouyang Song,Zhen Yu,Yajing You,Chenxi Liu,Xiaoyu Xie,Huanran Lv,Feng Xiao,Qiang Zhu,Chengyong QinPubMed 35428350
- Cell biology international 46:907-9212022PDGF-BB/PDGFRβ promotes epithelial-mesenchymal transition by affecting PI3K/AKT/mTOR-driven aerobic glycolysis in Wilms' tumor G401 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia-Qi Guo,Chang-Dong Wang,Hu-Ying Tang,Bo-Tao Sang,Xing Liu,Fa-Ping Yi,Xiang-Mei WuPubMed 35165984
- Biochemistry and cell biology = Biochimie et biologie cellulaire 100:75-842021PDGFRβ modulates aerobic glycolysis in osteosarcoma HOS cells via the PI3K/AKT/mTOR/c-Myc pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHu-Ying Tang,Jia-Qi Guo,Bo-Tao Sang,Jun-Ning Cheng,Xiang-Mei WuPubMed 34678088
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| Dot | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
The protein expressed by the PDGFRB gene is a tyrosine-protein kinase that functions as a cell-surface receptor for various PDGF homodimers and heterodimers, playing an essential role in embryonic development, cell proliferation, survival, differentiation, chemotaxis, and migration. It is crucial for blood vessel development by promoting the proliferation and migration of, as well as recruitment of, pericytes and smooth muscle cells to endothelial cells. It has a role in vascular smooth muscle cell migration and neointima formation at injury sites, and is necessary for normal cardiovascular development and pericyte recruitment in kidney glomeruli. The protein facilitates actin cytoskeleton rearrangement and membrane ruffle formation. Ligand binding activates signaling cascades depending on the ligand bound and interactions with PDGFRA, leading to phosphorylation of several substrates, activation of signaling pathways such as AKT1, MAPK, and SRC family kinases, and production of molecules like diacylglycerol and inositol trisphosphate. Receptor signaling is downregulated by protein phosphatases and receptor internalization. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
CD140b, PDGFR, PDGFR1, PDGFRB, Platelet-derived growth factor receptor beta, PDGF-R-beta, PDGFR-beta, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1
Recommended products
Rabbit Recombinant Monoclonal PDGFR beta phospho Y751 antibody. Suitable for Dot, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR21744
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDGFR beta also known as PDGFR-b or PDGFRB is a cell surface tyrosine kinase receptor with a molecular mass of about 180 kDa. It binds the PDGF (platelet-derived growth factor) family of ligands. PDGFR beta is commonly found in various tissues including blood vessels and connective tissues and is highly expressed in cells like pericytes and fibroblasts. The receptor plays a critical role in cell signaling mechanisms involving proliferation chemotaxis and survival.
- Biological function summary
The receptor plays an essential role in the regulation of cell growth and development. PDGFR beta undergoes dimerization and autophosphorylation upon ligand binding initiating a series of downstream signaling cascades. This receptor is often part of a complex with other receptor proteins promoting interactions necessary for signal propagation. Its main biological functions include mediating cellular responses to environmental signals that contribute to tissue repair and angiogenesis.
- Pathways
PDGFR beta is an important player within the PI3K-Akt and MAPK signaling pathways. It works alongside proteins such as PI3K and Ras to regulate cellular responses related to growth and survival. These pathways facilitate cross-talk with other cellular processes influencing various cellular outcomes. This receptor's activity regulates critical physiological functions by providing signals that maintain cellular homeostasis under various physiological conditions.
- Associated diseases and disorders
PDGFR beta has significant implications in the progression of cancer and fibrotic diseases. Its overexpression or mutation can lead to anomalous signaling that contributes to tumorigenesis particularly in connective tissue tumors known as sarcomas. Additionally PDGFR beta's role in promoting fibroblast activity makes it relevant in fibrotic diseases such as pulmonary fibrosis. Abnormal activation of PDGFR beta can interact with related proteins like VEGF receptors enhancing pathogenic responses and contributing to disease severity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (ab218534), expandable thumbnail](https://content.abcam.com/products/images/pdgfr-beta-phospho-y751-antibody-epr21744-ab218534--western-blot-img170563.jpg "Western blot - Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (ab218534)")
Western blot - Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (ab218534)
Expression of PDGFR beta (phospho Y751) is induced by PDGF-BB. The antibody recognizes both the glycosylated and unglycosylated protein (PMID: 9307305).
Exposure:
Lane 1 - 4: 80 seconds
Lane 5 - 6: 180 seconds
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (ab218534) at 1/1000 dilution
Lane 1: Untreated IMR-90 (human lung fibroblast cell line) whole cell lysate at 20 µg
Lane 2: IMR-90 treated with 50 ng/ml PDGF-BB for 5 minutes whole cell lysate at 20 µg
Lane 3: Untreated NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg
Lane 4: NIH/3T3 treated with 50 ng/ml PDGF-BB for 5 minutes whole cell lysate at 20 µg
Lane 5: Untreated C6 (rat glial tumor glial cell line) whole cell lysate at 20 µg
Lane 6: C6 treated with 100 ng/ml PDGF-BB for 60 minutes whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 123 kDa
Observed band size: 190 kDa
![Dot Blot - Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (ab218534), expandable thumbnail](https://content.abcam.com/products/images/pdgfr-beta-phospho-y751-antibody-epr21744-ab218534--dot-blot-img12632.jpg "Dot Blot - Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (ab218534)")
Dot Blot - Anti-PDGFR beta (phospho Y751) antibody [EPR21744] (ab218534)
Dot blot analysis of PDGFR beta (phospho Y751) labeled with ab218534 at 1/1000 dilution.
Lane 1: Human PDGFR beta (phospho Y751) peptide.
Lane 2: Human PDGFR beta non-phospho peptide.Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com