Name: Anti-PDHA1 antibody [9H9AF5]
SKU: ab110330
Rating: 5 (8 reviews)

Anti-PDHA1 antibody [9H9AF5] (ab110330) is a mouse monoclonal antibody that is used to detect PDHA1 in Western Blot, Flow Cytometry, ICC/IF. Suitable for Cow, Human, Mouse, Rat samples.

- Specificity confirmed with PDHA1 knockout cell line validation

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Western blot - Anti-PDHA1 antibody [9H9AF5] (AB110330), expandable thumbnail

Publications

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Flow Cyt	WB	ICC/IF
Human	Tested	Tested	Tested
Mouse	Expected	Tested	Expected
Rat	Expected	Tested	Expected
Cow	Expected	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1 µg/mL	Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Cow	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1 µg/mL	Notes -
Species Mouse	Dilution info 1 µg/mL	Notes -
Species Rat	Dilution info 1 µg/mL	Notes -
Species Cow	Dilution info 1 µg/mL	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 5 µg/mL	Notes (heat-induced antigen-retrieval improves signal)

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Cow	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information PDHA1

Function

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

Alternative names

PHE1A, PDHA1, PDHE1-A type I

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: 9H9AF5
Purity: IgG fraction
Light chain type: kappa
Concentration
Purification notes: ab110330 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

What is this antibody validated in?

Anti-PDHA1 antibody [9H9AF5] (ab110330) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Cow, Human, Mouse, Rat samples.

What is the molecular weight of PDHA1?

Anti-PDHA1 [9H9AF5] (ab110330) specifically detects a band for PDHA1 (UniProt: P08559) at a molecular weight of 43kDa.

Trusted by the scientific community

Anti-PDHA1 [9H9AF5] (ab110330) was first used in a scientific publication in 2011 and has been cited over 80 times in peer-reviewed journals.

Reviewed by scientists

Anti-PDHA1 [9H9AF5] (ab110330) has over 5 independent reviews from customers.

Specificity confirmed

The specificity of Anti-PDHA1 antibody [9H9AF5] (ab110330) has been confirmed by Western blot testing in PDHA1 Knockout HeLa cells.

Other related products

We have a range of other formats of antibody clone [9H9AF5] also available for your convenience:
ab110330, HRP - ab197956

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PDHA1 also known as the pyruvate dehydrogenase E1 alpha subunit plays a mechanical role in cellular metabolism. It forms part of the larger pyruvate dehydrogenase (PDH) complex where it serves as a critical catalytic component. PDHA1 is expressed ubiquitously across different tissue types reflecting its fundamental function in energy production. The molecular weight of the PDHA1 protein is approximately 43 kDa. Alternate names for this protein include the PDH E1 component and it partners closely with other components in the PDH complex to facilitate its role.

Biological function summary

PDHA1 engages in the conversion of pyruvate into acetyl-CoA an important step in cellular respiration. This protein is part of the PDH complex which consists of multiple copies of three catalytic and two regulatory subunits. The conversion process is essential for linking glycolysis to the citric acid cycle efficiently channeling energy substrates within the cell. Furthermore the functional activity of PDHA1 is regulated through phosphorylation by the pyruvate dehydrogenase kinases (PDKs) and dephosphorylation by PDH phosphatases.

Pathways

PDHA1 is integral to the metabolic pathway of cellular respiration and energy production. It enables the transition between glycolysis and the citric acid cycle by facilitating the conversion of pyruvate to acetyl-CoA which enters the citric acid cycle. Related proteins in this pathway include PDHA2 and the regulatory PDKs that modulate PDHA1 activity. These interactions ensure energy metabolism adapts to various cellular conditions influencing energy balance and substrate utilization.

Associated diseases and disorders

Mutations or dysfunctions in PDHA1 can lead to disorders such as pyruvate dehydrogenase deficiency and Leigh syndrome. These conditions result from impaired energy metabolism leading to severe neurological symptoms and overall energy deficits in tissues with high metabolic demands. The link between PDHA1 and diseases highlights the importance of maintaining its function. Additionally altered interaction with proteins involved in phosphorylation such as the PDKs can exacerbate pathogenic conditions by further disbalancing metabolic activities.

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6 product images

Western blot - Anti-PDHA1 antibody [9H9AF5] (ab110330)
Lanes 1 - 3: Merged signal (red and green). Green - ab110330 observed at 43 kDa. Red - loading control, Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866, observed at 50 kDa.
ab110330 was shown to specifically react with PDHA1 in wild-type HeLa cells as signal was lost in PDHA1 knockout cells. Wild-type and PDHA1 knockout samples were subjected to SDS-PAGE. ab110330 and Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1 ug/ml and 1/1000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^®680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PDHA1 antibody [9H9AF5] (ab110330) at 1 µg/mL
Lane 1: Wild-type HeLa whole cell lysate at 20 µg
Lane 2: PDHA1 knockout HeLa whole cell lysate at 20 µg
Lane 3: HEK-293 whole cell lysate at 20 µg
Predicted band size: 43 kDa
Observed band size: 43 kDa
Western blot - Anti-PDHA1 antibody [9H9AF5] (ab110330)
All lanes: Western blot - Anti-PDHA1 antibody [9H9AF5] (ab110330) at 1 µg/mL
Lane 1: Isolated mitochondria from Human heart at 10 µg
Lane 2: Isolated mitochondria from Bovine heart at 4 µg
Lane 3: Isolated mitochondria from Rat heart at 10 µg
Lane 4: Isolated mitochondria from Mouse heart at 10 µg
Lane 5: hepG2 cell lysate at 20 µg
Predicted band size: 43 kDa
'This image is courtesy of a customer review submitted by George Allen'
Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [9H9AF5] (ab110330)
Immunocytochemical analysis of SH-SY5Y human neuroblastoma labeling PDHA1 with ab110330 at 1/500 dilution.
Flow Cytometry - Anti-PDHA1 antibody [9H9AF5] (ab110330)
Flow cytometric analysis using ab110330 at 1μg/ml staining PDHA1 in HL60 cells (blue). Isotype control antibody (red).
Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [9H9AF5] (ab110330)
Immunocytochemistry analysis using ab110330 at 5μg/ml staining PDHA1 in cultured, normal Human embryonic lung fibroblasts (strain MRC5) followed by AlexaFluor® 488 goat anti-mouse IgG1 secondary antibody (2 μg/ml).
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-PDHA1 antibody [9H9AF5] (ab110330)
PDHA1 western blot using anti-PDHA1 antibody [9H9AF5] ab110330. Publication image and figure legend from Zhang, Y., Zhao, Z., et al., 2016, PLoS One, PubMed 26930489.

ab110330 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab110330 please see the product overview.
Induction of PDH phosphorylation by ATP.A. PDH phosphorylation. Mitochondria lysate was incubated with ATP at multiple dosages for 30 minutes at 37°C. PDH phosphorylation was quantified in Western blot and the results are expressed in bar figure B. Protein levels of PDKs. The proteins of PDK2 and PDK4 were determined in Western blot and the signals were quantified. The experiments were performed three times with consistent results, and the representative blot is shown. In the bar figure, the result represents mean ± SE (n = 3). * p<0.05 compared with the control.

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Anti-PDHA1 antibody [9H9AF5]

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