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Rabbit Polyclonal PDHA1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PDHA1 aa 1-300.

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Images

Western blot - Anti-PDHA1 antibody (AB97352), expandable thumbnail
  • Western blot - Anti-PDHA1 antibody (AB97352), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody (AB97352), expandable thumbnail

Publications

  • The Journal of biological chemistry 286:18383-962011
    Differences between human and rodent pancreatic islets: low pyruvate carboxylase, atp citrate lyase, and pyruvate carboxylation and high glucose-stimulated acetoacetate in human pancreatic islets.
    Applications:
    WB
    Reactive species:
    Rat, Human
    Michael J MacDonald et. al.
    PubMed 21454710

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human PDHA1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P08559

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Cow
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/200

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

Alternative names

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Rabbit Polyclonal PDHA1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PDHA1 aa 1-300.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human PDHA1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P08559
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Activity summary

PDHA1 also known as the pyruvate dehydrogenase E1 alpha subunit plays a mechanical role in cellular metabolism. It forms part of the larger pyruvate dehydrogenase (PDH) complex where it serves as a critical catalytic component. PDHA1 is expressed ubiquitously across different tissue types reflecting its fundamental function in energy production. The molecular weight of the PDHA1 protein is approximately 43 kDa. Alternate names for this protein include the PDH E1 component and it partners closely with other components in the PDH complex to facilitate its role.

Biological function summary

PDHA1 engages in the conversion of pyruvate into acetyl-CoA an important step in cellular respiration. This protein is part of the PDH complex which consists of multiple copies of three catalytic and two regulatory subunits. The conversion process is essential for linking glycolysis to the citric acid cycle efficiently channeling energy substrates within the cell. Furthermore the functional activity of PDHA1 is regulated through phosphorylation by the pyruvate dehydrogenase kinases (PDKs) and dephosphorylation by PDH phosphatases.

Pathways

PDHA1 is integral to the metabolic pathway of cellular respiration and energy production. It enables the transition between glycolysis and the citric acid cycle by facilitating the conversion of pyruvate to acetyl-CoA which enters the citric acid cycle. Related proteins in this pathway include PDHA2 and the regulatory PDKs that modulate PDHA1 activity. These interactions ensure energy metabolism adapts to various cellular conditions influencing energy balance and substrate utilization.

Associated diseases and disorders

Mutations or dysfunctions in PDHA1 can lead to disorders such as pyruvate dehydrogenase deficiency and Leigh syndrome. These conditions result from impaired energy metabolism leading to severe neurological symptoms and overall energy deficits in tissues with high metabolic demands. The link between PDHA1 and diseases highlights the importance of maintaining its function. Additionally altered interaction with proteins involved in phosphorylation such as the PDKs can exacerbate pathogenic conditions by further disbalancing metabolic activities.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-PDHA1 antibody (ab97352), expandable thumbnail

    Western blot - Anti-PDHA1 antibody (ab97352)

    All lanes: Western blot - Anti-PDHA1 antibody (ab97352) at 1/1000 dilution

    Lane 1: WT HeLa cell extract at 30 µg

    Lane 2: Pyruvate Dehydrogenase E1-alpha subunit Knockout HeLa cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG

    Predicted band size: 43 kDa

  • Western blot - Anti-PDHA1 antibody (ab97352), expandable thumbnail

    Western blot - Anti-PDHA1 antibody (ab97352)

    12% SDS PAGE

    All lanes: Western blot - Anti-PDHA1 antibody (ab97352) at 1/1000 dilution

    Lane 1: HepG2 whole cell lysate at 30 µg

    Lane 2: MOLT4 whole cell lysate at 30 µg

    Predicted band size: 43 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody (ab97352), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody (ab97352)

    ab97352, at a 1/200 dilution, staining PDHA1 in paraformaldehyde fixed HeLa cells by Immunofluorescence. Lower image was merged with DNA probe.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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