AB97352

Anti-PDHA1 antibody

Name: Anti-PDHA1 antibody (ab97352) | Abcam
Brand: Abcam Limited
SKU: AB97352
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal PDHA1 antibody. Suitable for IHC - Wmt, IHC-P, IP, WB, ICC/IF and reacts with Zebrafish, Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PDHA1 aa 1-300.

View Alternative Names

PHE1A, PDHA1, PDHE1-A type I

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody (AB97352)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody (AB97352)

Immunohistochemical analysis of paraffin-embedded hepatoma tissue stained for PDHA1 protein at cytosol on human hepatoma with ab97352 at a 1/500 dilution.

Antigen Retrieval : EDTA based, pH 8.0 buffer, 15min.

Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody (AB97352)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody (AB97352)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling Pyruvate Dehydrogenase E1 alpha protein at mitochondria using ab97352 at a 1/2000 dilution (Green). DAPI was used for nuclear staining.

Supplier Data

Immunoprecipitation - Anti-PDHA1 antibody (AB97352)

Immunoprecipitation of Pyruvate Dehydrogenase E1 alpha protein from HepG2 whole cell extracts using 5 µg of Pyruvate Dehydrogenase E1 alpha antibody.
Western blot analysis was performed using Pyruvate Dehydrogenase E1 alpha antibody.

All lanes:

Immunoprecipitation - Anti-PDHA1 antibody (ab97352) at 5 µg

Lane 1:

HepG2 whole cell extracts (Input)

Lane 2:

HepG2 whole cell extracts with control (-)

Lane 3:

HepG2 whole cell extracts (+)

Secondary

All lanes:

Anti-Rabbit IgG Antibody

Observed band size: 43 kDa

false

Supplier Data

Western blot - Anti-PDHA1 antibody (AB97352)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-PDHA1 antibody (ab97352) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 43 kDa

false

Supplier Data

Western blot - Anti-PDHA1 antibody (AB97352)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PDHA1 antibody (ab97352) at 1/1000 dilution

Lane 1:

WT HeLa cell extracts at 30 µg

Lane 2:

KO HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 43 kDa

false

Supplier Data

Western blot - Anti-PDHA1 antibody (AB97352)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PDHA1 antibody (ab97352) at 1/3000 dilution

All lanes:

Rat brain lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 43 kDa

false

Supplier Data

Western blot - Anti-PDHA1 antibody (AB97352)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PDHA1 antibody (ab97352) at 1/5000 dilution

All lanes:

mouse brain lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 43 kDa

false

IHC - Wholemount - Anti-PDHA1 antibody (AB97352)

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-PDHA1 antibody (AB97352)

ab97352 detects Pyruvate Dehydrogenase E1 alpha protein on whole mount zebrafish by immunohistochemical analysis.
Sample : Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.
Green : Pyruvate Dehydrogenase E1 alpha stained by ab97352 diluted at 1/100 dilution.

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-PDHA1 antibody (AB97352)

Immunohistochemical analysis (whole mount) of zebrafish embryo, using ab97352 at 1/200 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Zebrafish

Applications

IHC - Wmt, WB, IHC-P, ICC/IF, IP

applications

Immunogen

Recombinant Fragment Protein within Human PDHA1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P08559

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDHA1 also known as the pyruvate dehydrogenase E1 alpha subunit plays a mechanical role in cellular metabolism. It forms part of the larger pyruvate dehydrogenase (PDH) complex where it serves as a critical catalytic component. PDHA1 is expressed ubiquitously across different tissue types reflecting its fundamental function in energy production. The molecular weight of the PDHA1 protein is approximately 43 kDa. Alternate names for this protein include the PDH E1 component and it partners closely with other components in the PDH complex to facilitate its role.

Biological function summary

PDHA1 engages in the conversion of pyruvate into acetyl-CoA an important step in cellular respiration. This protein is part of the PDH complex which consists of multiple copies of three catalytic and two regulatory subunits. The conversion process is essential for linking glycolysis to the citric acid cycle efficiently channeling energy substrates within the cell. Furthermore the functional activity of PDHA1 is regulated through phosphorylation by the pyruvate dehydrogenase kinases (PDKs) and dephosphorylation by PDH phosphatases.

Pathways

PDHA1 is integral to the metabolic pathway of cellular respiration and energy production. It enables the transition between glycolysis and the citric acid cycle by facilitating the conversion of pyruvate to acetyl-CoA which enters the citric acid cycle. Related proteins in this pathway include PDHA2 and the regulatory PDKs that modulate PDHA1 activity. These interactions ensure energy metabolism adapts to various cellular conditions influencing energy balance and substrate utilization.

Mutations or dysfunctions in PDHA1 can lead to disorders such as pyruvate dehydrogenase deficiency and Leigh syndrome. These conditions result from impaired energy metabolism leading to severe neurological symptoms and overall energy deficits in tissues with high metabolic demands. The link between PDHA1 and diseases highlights the importance of maintaining its function. Additionally altered interaction with proteins involved in phosphorylation such as the PDKs can exacerbate pathogenic conditions by further disbalancing metabolic activities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Together with PDHB forms the heterotetrameric E1 subunit of the pyruvate dehydrogenase (PDH) complex (PubMed : 17474719, PubMed : 19081061). The PDH complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle (PubMed : 19081061, PubMed : 7782287). It contains multiple copies of three enzymatic components : pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3) (Probable). The E1 subunit catalyzes both the thiamine pyrophosphate (TPP)-dependent decarboxylation of pyruvate and the reductive acetylation of a lipoyl group covalently linked to the lipoyl-bearing domains of E2 (PubMed : 17474719, PubMed : 19081061, PubMed : 7782287).

See full target information PDHA1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 286:18383-96 PubMed21454710

2011

Differences between human and rodent pancreatic islets: low pyruvate carboxylase, atp citrate lyase, and pyruvate carboxylation and high glucose-stimulated acetoacetate in human pancreatic islets.

Applications

WB, WB

Species

Rat, Human

Michael J MacDonald,Melissa J Longacre,Scott W Stoker,Mindy Kendrick,Ansaya Thonpho,Laura J Brown,Noaman M Hasan,Sarawut Jitrapakdee,Toshiyuki Fukao,Matthew S Hanson,Luis A Fernandez,Jon Odorico

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDHA1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody (AB97352)

Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody (AB97352)

Immunoprecipitation - Anti-PDHA1 antibody (AB97352)

Western blot - Anti-PDHA1 antibody (AB97352)

Western blot - Anti-PDHA1 antibody (AB97352)

Western blot - Anti-PDHA1 antibody (AB97352)

Western blot - Anti-PDHA1 antibody (AB97352)

IHC - Wholemount - Anti-PDHA1 antibody (AB97352)

IHC - Wholemount - Anti-PDHA1 antibody (AB97352)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise