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Rabbit Recombinant Monoclonal PDHA1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 33 publications.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Tested
Rat
Expected
Tested
Expected
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/10 - 1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000 - 1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000 - 1/5000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/500

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10 - 1/100

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

Alternative names

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Rabbit Recombinant Monoclonal PDHA1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 33 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR11098

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

PDHA1 engages in the conversion of pyruvate into acetyl-CoA an important step in cellular respiration. This protein is part of the PDH complex which consists of multiple copies of three catalytic and two regulatory subunits. The conversion process is essential for linking glycolysis to the citric acid cycle efficiently channeling energy substrates within the cell. Furthermore the functional activity of PDHA1 is regulated through phosphorylation by the pyruvate dehydrogenase kinases (PDKs) and dephosphorylation by PDH phosphatases.

Activity summary

PDHA1 also known as the pyruvate dehydrogenase E1 alpha subunit plays a mechanical role in cellular metabolism. It forms part of the larger pyruvate dehydrogenase (PDH) complex where it serves as a critical catalytic component. PDHA1 is expressed ubiquitously across different tissue types reflecting its fundamental function in energy production. The molecular weight of the PDHA1 protein is approximately 43 kDa. Alternate names for this protein include the PDH E1 component and it partners closely with other components in the PDH complex to facilitate its role.

Pathways

PDHA1 is integral to the metabolic pathway of cellular respiration and energy production. It enables the transition between glycolysis and the citric acid cycle by facilitating the conversion of pyruvate to acetyl-CoA which enters the citric acid cycle. Related proteins in this pathway include PDHA2 and the regulatory PDKs that modulate PDHA1 activity. These interactions ensure energy metabolism adapts to various cellular conditions influencing energy balance and substrate utilization.

Associated diseases and disorders

Mutations or dysfunctions in PDHA1 can lead to disorders such as pyruvate dehydrogenase deficiency and Leigh syndrome. These conditions result from impaired energy metabolism leading to severe neurological symptoms and overall energy deficits in tissues with high metabolic demands. The link between PDHA1 and diseases highlights the importance of maintaining its function. Additionally altered interaction with proteins involved in phosphorylation such as the PDKs can exacerbate pathogenic conditions by further disbalancing metabolic activities.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

  • Immunoprecipitation - Anti-PDHA1 antibody [EPR11098] (ab168379), expandable thumbnail

    Immunoprecipitation - Anti-PDHA1 antibody [EPR11098] (ab168379)

    ab168379 (purified) at 1:20 dilution (2ug) immunoprecipitating PDHA1 in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab168379 & HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab168379 in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-PDHA1 antibody [EPR11098] (AB168379)

    Predicted band size: 43 kDa

  • Western blot - Anti-PDHA1 antibody [EPR11098] (ab168379), expandable thumbnail

    Western blot - Anti-PDHA1 antibody [EPR11098] (ab168379)

    Lanes 1 - 3: Merged signal (red and green). Green - ab168379 observed at 43 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab168379 was shown to recognize PDHA1 in wild-type A549 cells as signal was lost at the expected MW in PDHA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PDHA1 knockout samples were subjected to SDS-PAGE. Ab168379 and ab130007 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-PDHA1 antibody [EPR11098] (AB168379) at 1/1000 dilution

    Lane 1: Wild-type A549 whole cell lysate at 20 µg

    Lane 2: PDHA1 knockout A549 whole cell lysate at 20 µg

    Lane 3: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 43 kDa

    Observed band size: 43 kDa

  • Western blot - Anti-PDHA1 antibody [EPR11098] (ab168379), expandable thumbnail

    Western blot - Anti-PDHA1 antibody [EPR11098] (ab168379)

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-PDHA1 antibody [EPR11098] (AB168379) at 1/2000 dilution

    Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 2: Mouse brain lysates at 20 µg

    Lane 3: Rat brain lysates at 20 µg

    Lane 4: Mouse kidney lysates at 20 µg

    Lane 5: Rat kidney lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 43 kDa

    Observed band size: 43 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] (ab168379), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] (ab168379)

    Immunocytochemistry/ Immunofluorescence analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling PDHA1 with Purified ab168379 at 1:100 dilution (3.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-PDHA1 antibody [EPR11098] (ab168379), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PDHA1 antibody [EPR11098] (ab168379)

    Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling PDHA1 with purified ab168379 at 1/40 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] (ab168379), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] (ab168379)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling PDHA1 with Purified ab168379 at 1:200 dilution (1.76 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] (ab168379), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] (ab168379)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling PDHA1 with Purified ab168379 at 1:200 dilution (1.76 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] (ab168379), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] (ab168379)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling PDHA1 with Purified ab168379 at 1:200 dilution (1.76 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [EPR11098] (ab168379), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [EPR11098] (ab168379)

    Immunocytochemistry/Immunofluorescence analysis Jurkat (human acute T cell leukemia) labelling PDHA1 with purified ab168379 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

  • Western blot - Anti-PDHA1 antibody [EPR11098] (ab168379), expandable thumbnail

    Western blot - Anti-PDHA1 antibody [EPR11098] (ab168379)

    All lanes: Western blot - Anti-PDHA1 antibody [EPR11098] (AB168379) at 1/1000 dilution

    Lane 1: Human fetal kidney lysate at 10 µg

    Lane 2: A549 lysate at 10 µg

    Lane 3: Jurkat lysate at 10 µg

    Lane 4: HepG2 lysate at 10 µg

    Lane 5: HeLa lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 43 kDa

  • Immunoprecipitation - Anti-PDHA1 antibody [EPR11098] (ab168379), expandable thumbnail

    Immunoprecipitation - Anti-PDHA1 antibody [EPR11098] (ab168379)

    Detection of PDHA1 by Western Blot of Immunprecipitate. 293T cell lysate immunoprecipitated using unpurified ab168379 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    All lanes: Immunoprecipitation - Anti-PDHA1 antibody [EPR11098] (AB168379)

    Predicted band size: 43 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] (ab168379), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] (ab168379)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PDHA1 with unpurified ab168379 at 1/100 dilution. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] (ab168379), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11098] (ab168379)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PDHA1 with unpurified ab168379 at 1/100 dilution. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [EPR11098] (ab168379), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PDHA1 antibody [EPR11098] (ab168379)

    Immunofluorescent analysis of HepG2 cells labeling PDHA1 with unpurified ab168379 at 1/100 dilution.

  • Flow Cytometry (Intracellular) - Anti-PDHA1 antibody [EPR11098] (ab168379), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PDHA1 antibody [EPR11098] (ab168379)

    Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling PDHA1 (red) with unpurified ab168379 at 1/10 dilution, or a rabbit IgG (negative) (green).

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Product protocols

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