Name: Anti-PDHA1 (phospho S293) antibody [EPR12200]
SKU: ab177461
Rating: 5 (2 reviews)

Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461) is a rabbit monoclonal antibody that is used to detect PDHA1 in Western Blot, IP, IHC-P, ELISA. Suitable for Human, Mouse, Rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 50 publications

Images

Immunoprecipitation - Anti-PDHA1 (phospho S293) antibody [EPR12200] (AB177461), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	ELISA	WB	IHC-P
Human	Tested	Tested	Tested	Tested
Mouse	Expected	Expected	Tested	Tested
Rat	Expected	Expected	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/10 - 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000 - 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000 - 1/5000	Notes -
Species Human	Dilution info 1/1000 - 1/5000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/250	Notes For unpurified use at 1/50 - 1/100. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/250	Notes For unpurified use at 1/50 - 1/100. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/250	Notes For unpurified use at 1/50 - 1/100. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

See full target information PDHA1 phospho S293

Function

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

Alternative names

PHE1A, PDHA1, PDHE1-A type I

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR12200
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), ELISA, in Human, Mouse, Rat samples.

What is the molecular weight of PDHA1?

Anti-PDHA1 (phospho S293) [EPR12200] (ab177461) specifically detects a band for PDHA1 (UniProt: P08559) at a molecular weight of 40kDa.

Recommended positive controls

WB: HEK-293T cell lysates; Rat kidney lysate; HT-29 cell lysate.IP: HEK-293T cell lysates.IHC-P: Human breast and colon tissue. Rat and mouse colon tissue.

Trusted by the scientific community

Anti-PDHA1 (phospho S293) [EPR12200] (ab177461) was first used in a scientific publication in 2013 and has been cited over 50 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products

We have a range of other formats of antibody clone [EPR12200] also available for your convenience:
ab177461, Carrier free - ab227565

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PDHA1 also known as the pyruvate dehydrogenase E1 alpha subunit plays a mechanical role in cellular metabolism. It forms part of the larger pyruvate dehydrogenase (PDH) complex where it serves as a critical catalytic component. PDHA1 is expressed ubiquitously across different tissue types reflecting its fundamental function in energy production. The molecular weight of the PDHA1 protein is approximately 43 kDa. Alternate names for this protein include the PDH E1 component and it partners closely with other components in the PDH complex to facilitate its role.

Biological function summary

PDHA1 engages in the conversion of pyruvate into acetyl-CoA an important step in cellular respiration. This protein is part of the PDH complex which consists of multiple copies of three catalytic and two regulatory subunits. The conversion process is essential for linking glycolysis to the citric acid cycle efficiently channeling energy substrates within the cell. Furthermore the functional activity of PDHA1 is regulated through phosphorylation by the pyruvate dehydrogenase kinases (PDKs) and dephosphorylation by PDH phosphatases.

Pathways

PDHA1 is integral to the metabolic pathway of cellular respiration and energy production. It enables the transition between glycolysis and the citric acid cycle by facilitating the conversion of pyruvate to acetyl-CoA which enters the citric acid cycle. Related proteins in this pathway include PDHA2 and the regulatory PDKs that modulate PDHA1 activity. These interactions ensure energy metabolism adapts to various cellular conditions influencing energy balance and substrate utilization.

Associated diseases and disorders

Mutations or dysfunctions in PDHA1 can lead to disorders such as pyruvate dehydrogenase deficiency and Leigh syndrome. These conditions result from impaired energy metabolism leading to severe neurological symptoms and overall energy deficits in tissues with high metabolic demands. The link between PDHA1 and diseases highlights the importance of maintaining its function. Additionally altered interaction with proteins involved in phosphorylation such as the PDKs can exacerbate pathogenic conditions by further disbalancing metabolic activities.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

Immunoprecipitation - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461)
ab177461 (purified) at 1:20 dilution (2μg) immunoprecipitating PDHA1 in HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate.
Lane 1 (input): HEK-293T whole cell lysate 10μg
Lane 2 (+): ab177461 & HEK-293T whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab177461 in HEK-293T whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent(HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461)
Predicted band size: 43 kDa
Western blot - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461)
Blocking and diluting buffer : 5% NFDM/TBST
All lanes: Western blot - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461) at 1/2000 dilution
Lane 1: Rat kidney lysates at 15 µg
Lane 2: Rat kidney lysates.Then the membrane was incubated with phosphatase. at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue sections labeling PDHA1 with Purified ab177461 at 1:250 dilution (0.52 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue sections labeling PDHA1 with Purified ab177461 at 1:250 dilution (0.52 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling PDHA1 with Purified ab177461 at 1:250 dilution (0.52 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461)
Blocking and diluting buffer : 5% NFDM/TBST
All lanes: Western blot - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461) at 1/2000 dilution
Lane 1: Mouse kidney lysates at 15 µg
Lane 2: Mouse kidney lysates.Then the membrane was incubated with phosphatase. at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Western blot - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461)
Blocking and diluting buffer : 5% NFDM/TBST
All lanes: Western blot - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461) at 1/2000 dilution
Lane 1: HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2: HT-29 (Human colorectal adenocarcinoma epithelial cell) treated with 8 mM Sodium butyrate for 24 hours whole cell lysates at 15 µg
Lane 3: HT-29 (Human colorectal adenocarcinoma epithelial cell) treated with 8 mM Sodium butyrate for 24 hours whole cell lysates.Then the membrane was incubated with phosphatase at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
ELISA - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461)
Serially diluted unpurified ab177461 was bound to immobilised human phospho peptide (S293) - or Control peptide (1 microgram x mL-1). The antibody was detected by HRP-labelled goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080; diluted 50000 times) and signal was developed with TMB substrate.
Western blot - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour and then treated with either buffer (lane 1) or alkaline phosphatase (lane 2), before being incubated with ab177461 overnight at 4°C. Antibody binding was detected using IR-labelled goat anti-rabbit (green) and goat anti-mouse (Red) at 1:10,000 dilution for one hour at room temperature before imaging.
All lanes: Western blot - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461) at 1/1000 dilution
All lanes: HeLa Whole Cell Lysate + Calyculin A (30nM for 20min) at 20 µg
Secondary
All lanes: goat anti-rabbit (green) and goat anti-mouse at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 43 kDa
Western blot - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461)
All lanes: Western blot - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461) at 1/1000 dilution
Lane 1: Immunoprecipitation pellet from HEK-293T cell lysate at 10 µg
Lane 2: 1X PBS (negative control)
Secondary
All lanes: Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 43 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling PDHA1 with unpurified ab177461 at 1/50 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461)
Immunohistochemical analysis of paraffin-embedded human breast tissue labeling PDHA1 with unpurified ab177461 at 1/50 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461)
All lanes: Western blot - Anti-PDHA1 (phospho S293) antibody [EPR12200] (ab177461) at 1/1000 dilution
Lane 1: HEK-293T cell lysates untreated at 10 µg
Lane 2: HEK-293T cell lysates, membrane treated with Lambda Phosphatase at 10 µg
Secondary
All lanes: Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 43 kDa