AB110331

Anti-PDHB antibody [17A5E2H8]

Name: Anti-PDHB antibody [17A5E2H8] (ab110331) | Abcam
Brand: Abcam Limited
SKU: AB110331
Price: 525 USD
Availability: InStock
Rating: 5 (1 reviews)

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What is this?

(1 Review)

(6 Publications)

Mouse Monoclonal PDHB antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat, Cow samples. Cited in 6 publications.

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [17A5E2H8] (AB110331)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [17A5E2H8] (AB110331)

IHC image of PDHB staining in Human normal duodenum formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110331, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Unknown

Western blot - Anti-PDHB antibody [17A5E2H8] (AB110331)

All lanes:

Western blot - Anti-PDHB antibody [17A5E2H8] (ab110331) at 1 µg/mL

Lane 1:

Isolated mitochondria from Human heart at 10 µg/mL

Lane 2:

Isolated mitochondria from Bovine heart at 4 µg/mL

Lane 3:

Isolated mitochondria from Rat heart at 10 µg/mL

Lane 4:

Isolated mitochondria from Mouse heart at 10 µg/mL

Lane 5:

HepG2 cell lysate at 20 µg/mL

Predicted band size: 39 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

17A5E2H8

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Mouse, Rat, Cow, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

ab110331 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDHB also known as pyruvate dehydrogenase beta subunit plays an important role in the conversion of pyruvate to acetyl-CoA. This protein forms a part of the pyruvate dehydrogenase complex (PDC) which occurs in mitochondria of numerous tissues. The PDHB subunit has a molecular mass of approximately 39 kDa. Expression of PDHB is not limited to one specific type of tissue it is generally found in various tissues indicating its widespread involvement in cellular metabolism.

Biological function summary

PDHB functions as an essential component of the PDC which catalyzes the decarboxylation of pyruvate linking glycolysis to the citric acid cycle. The PDC itself is a large multi-enzyme complex including other subunits such as E1 and E2 which coordinate efficiently to carry out its function. In this way PDHB contributes to energy production by enabling entry of carbon skeletons into the tricarboxylic acid cycle important for ATP generation.

Pathways

PDHB holds a significant role in central metabolic pathways namely glycolysis and the citric acid cycle. The conversion facilitated by the PDC forms a vital cornerstone for metabolic flux regulation influencing gluconeogenesis and fatty acid synthesis. PDHB also interacts with proteins such as pyruvate dehydrogenase kinase which regulates the PDC activity by phosphorylation thereby affecting energy metabolism directly.

Alterations in PDHB can lead to metabolic disorders such as pyruvate dehydrogenase deficiency potentially causing neurological dysfunction due to impaired energy metabolism. PDHB mutations also have a link to mitochondrial diseases reflecting its significant role in energy homeostasis. In these contexts proteins like the pyruvate dehydrogenase phosphatase are involved highlighting the clinical relevance of PDHB alterations in maintaining proper metabolic health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information PDHB

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 15:982-994 PubMed36915794

2023

Beta-catenin knockdown impairs the viability of ovarian cancer cells by modulating YAP-dependent glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Shuang Jia,Ling Yang,Yong-Qing Zhu,Cheng-Bin Ma

Visual neuroscience 36:E007 PubMed31199213

2019

Mitochondrial absorption of short wavelength light drives primate blue retinal cones into glycolysis which may increase their pace of aging.

Applications

Unspecified application

Species

Unspecified reactive species

Jaimie Hoh Kam,Tobias W Weinrich,Harpreet Sangha,Michael B Powner,Robert Fosbury,Glen Jeffery

Reproductive biomedicine online 29:761-70 PubMed25444511

2014

Knockdown of prohibitin expression promotes glucose metabolism in eutopic endometrial stromal cells from women with endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochen Qi,Yanxin Zhang,Hui Ji,Xiadi Wu,Fuxin Wang,Manxin Xie,Li Shu,Shiwen Jiang,Yundong Mao,Yugui Cui,Jiayin Liu

Glia 58:1168-76 PubMed20544852

2010

Phosphorylation status of pyruvate dehydrogenase distinguishes metabolic phenotypes of cultured rat brain astrocytes and neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Nader D Halim,Thomas Mcfate,Ahmed Mohyeldin,Peter Okagaki,Lioubov G Korotchkina,Mulchand S Patel,Nam Ho Jeoung,Robert A Harris,Michael J Schell,Ajay Verma

The International journal of developmental biology 52:463-72 PubMed18649259

2008

Identification of proteins undergoing tyrosine phosphorylation during mouse sperm capacitation.

Applications

Unspecified application

Species

Unspecified reactive species

Enid Arcelay,Ana M Salicioni,Eva Wertheimer,Pablo E Visconti

Molecular genetics and metabolism 93:381-7 PubMed18206410

2008

A combined therapeutic approach for pyruvate dehydrogenase deficiency using self-complementary adeno-associated virus serotype-specific vectors and dichloroacetate.

Applications

Unspecified application

Species

Unspecified reactive species

Zongchao Han,Kristen Berendzen,Li Zhong,Ira Surolia,Nitin Chouthai,Weihong Zhao,Njeri Maina,Arun Srivastava,Peter W Stacpoole

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDHB antibody [17A5E2H8]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [17A5E2H8] (AB110331)

Western blot - Anti-PDHB antibody [17A5E2H8] (AB110331)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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