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Rabbit Recombinant Monoclonal PDHB antibody. Suitable for IHC-P, Flow Cyt, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 13 publications.

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Images

Flow Cytometry - Anti-PDHB antibody [EPR11097(B)] (AB155996), expandable thumbnail
  • Western blot - Anti-PDHB antibody [EPR11097(B)] (AB155996), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (AB155996), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PDHB antibody [EPR11097(B)] (AB155996), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (AB155996), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWBICC/IF
Human
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Tested
Expected
Rat
Tested
Expected
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1500
Notes

For unpurified use at 1/50- 1/100.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1500
Notes

For unpurified use at 1/50- 1/100.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1500
Notes

For unpurified use at 1/50- 1/100.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/20
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/10000 - 1/50000
Notes

-

Species
Rat
Dilution info
1/10000 - 1/50000
Notes

-

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/250
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

Alternative names

PHE1B, PDHB, PDHE1-B

Recommended products

Rabbit Recombinant Monoclonal PDHB antibody. Suitable for IHC-P, Flow Cyt, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 13 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR11097(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PDHB also known as pyruvate dehydrogenase beta subunit plays an important role in the conversion of pyruvate to acetyl-CoA. This protein forms a part of the pyruvate dehydrogenase complex (PDC) which occurs in mitochondria of numerous tissues. The PDHB subunit has a molecular mass of approximately 39 kDa. Expression of PDHB is not limited to one specific type of tissue it is generally found in various tissues indicating its widespread involvement in cellular metabolism.

Biological function summary

PDHB functions as an essential component of the PDC which catalyzes the decarboxylation of pyruvate linking glycolysis to the citric acid cycle. The PDC itself is a large multi-enzyme complex including other subunits such as E1 and E2 which coordinate efficiently to carry out its function. In this way PDHB contributes to energy production by enabling entry of carbon skeletons into the tricarboxylic acid cycle important for ATP generation.

Pathways

PDHB holds a significant role in central metabolic pathways namely glycolysis and the citric acid cycle. The conversion facilitated by the PDC forms a vital cornerstone for metabolic flux regulation influencing gluconeogenesis and fatty acid synthesis. PDHB also interacts with proteins such as pyruvate dehydrogenase kinase which regulates the PDC activity by phosphorylation thereby affecting energy metabolism directly.

Associated diseases and disorders

Alterations in PDHB can lead to metabolic disorders such as pyruvate dehydrogenase deficiency potentially causing neurological dysfunction due to impaired energy metabolism. PDHB mutations also have a link to mitochondrial diseases reflecting its significant role in energy homeostasis. In these contexts proteins like the pyruvate dehydrogenase phosphatase are involved highlighting the clinical relevance of PDHB alterations in maintaining proper metabolic health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry - Anti-PDHB antibody [EPR11097(B)] (ab155996), expandable thumbnail

    Flow Cytometry - Anti-PDHB antibody [EPR11097(B)] (ab155996)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling PDHB with purified ab155996 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-PDHB antibody [EPR11097(B)] (ab155996), expandable thumbnail

    Western blot - Anti-PDHB antibody [EPR11097(B)] (ab155996)

    All lanes: Western blot - Anti-PDHB antibody [EPR11097(B)] (ab155996) at 1/10000 dilution

    Lane 1: A375 (Human malignant melanoma epithelial cell) whole cell lysate at 15 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: Mouse spleen lysate at 20 µg

    Lane 4: Mouse kidney lysate at 20 µg

    Lane 5: Rat kidney lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (ab155996), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (ab155996)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue sections labeling PDHB with purified ab155996 at 1/1500 dilution (0.08 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunocytochemistry/ Immunofluorescence - Anti-PDHB antibody [EPR11097(B)] (ab155996), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PDHB antibody [EPR11097(B)] (ab155996)

    Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling PDHB with purified ab155996 at 1/50 dilution (2.5 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (ab155996), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (ab155996)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue sections labeling PDHB with purified ab155996 at 1/1500 dilution (0.08 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (ab155996), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (ab155996)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue sections labeling PDHB with purified ab155996 at 1/1500 dilution (0.08 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

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Product protocols

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