AB223520

Anti-PDIA2/PDIP antibody

Name: Anti-PDIA2/PDIP antibody (ab223520) | Abcam
Brand: Abcam Limited
SKU: AB223520
Price: 525 USD
Availability: InStock

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(2 Publications)

Goat Polyclonal PDIA2/PDIP antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PDIA2 aa 450-500.

View Alternative Names

PDIP, PDIA2, Protein disulfide-isomerase A2, Pancreas-specific protein disulfide isomerase, PDIp

1 Images

Supplier Data

Western blot - Anti-PDIA2/PDIP antibody (AB223520)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-PDIA2/PDIP antibody (ab223520) at 0.1 µg/mL

Lane 1:

Human pancreas lysate (in RIPA buffer) at 35 µg

Lane 2:

Mouse pancreas lysate (in RIPA buffer) at 35 µg

Lane 3:

Rat pancreas lysate (in RIPA buffer) at 35 µg

Predicted band size: 58 kDa

Observed band size: 65 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human PDIA2 aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

Q13087

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3-5 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab223520 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDIA2 also known as PDIP is a protein disulfide isomerase with a prominent role in catalyzing protein folding through the isomerization of disulfide bonds. It is part of the broader family known as PDI proteins. PDIA2 has a mass of approximately 57 kDa and expression occurs mainly in the endoplasmic reticulum. Its enzymatic activity contributes to the maintenance of cellular homeostasis by ensuring proper protein conformation. Researchers have identified PDIA2 as a target for potential PDI inhibitors due to its critical function in protein folding.

Biological function summary

PDIA2 facilitates the correct formation of disulfide bonds which is essential for protein maturation and stability. This protein operates within a multi-component complex involved in oxidative protein folding regulating the redox state in the endoplasmic reticulum environment. By assisting in proper protein assembly PDIA2 maintains the equilibrium of protein synthesis folding and degradation suggesting its importance in cellular protein metabolism.

Pathways

Studies show that PDIA2 plays a role in the oxidative protein folding pathway which is part of the larger cellular response network to oxidative stress. The interaction of PDIA2 with other protein disulfide isomerases such as PDI and ERP57 highlights its role in this critical pathway. Furthermore PDIA2 is linked to the unfolded protein response pathway indicating its involvement in cell stress conditions where misfolded proteins accumulate.

PDIA2 has associations with metabolic disorders and neurodegenerative diseases. Its role in the proper folding of insulin and other secreted proteins links PDIA2 to diabetes-related conditions. Additionally research suggests connections between PDIA2 and neurodegenerative diseases like Alzheimer's where protein misfolding and aggregation play pivotal roles. PDIA2's relationship with proteins like amyloid precursor protein further corroborates its involvement in the pathological processes of such disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.

See full target information PDIA2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 12:836087 PubMed35860571

2022

PDIA2 Bridges Endoplasmic Reticulum Stress and Metabolic Reprogramming During Malignant Transformation of Chronic Colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Tao,Lin Yin,Ao Wu,Jiaoli Zhang,Jingpu Zhang,Huichun Shi,Siyuan Liu,Liangfei Niu,Li Xu,Yanling Feng,Shixian Lian,Lei Li,Liyan Zeng,Xianmin Meng,Xiaohui Zhou,Tiefu Liu,Lijun Zhang

Redox biology 24:101211 PubMed31108460

2019

Ferroptosis is governed by differential regulation of transcription in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhang,Lutao Du,Yongxia Qiao,Xiaobai Zhang,Weisheng Zheng,Qi Wu,Yan Chen,Guoqing Zhu,Ya Liu,Zhixuan Bian,Susu Guo,Yueyue Yang,Lifang Ma,Yongchun Yu,Qiuhui Pan,Fenyong Sun,Jiayi Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDIA2/PDIP antibody

Western blot - Anti-PDIA2/PDIP antibody (AB223520)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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