AB180931

Anti-PDIA2/PDIP antibody [EPR12212]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal PDIA2/PDIP antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PDIP, PDIA2, Protein disulfide-isomerase A2, Pancreas-specific protein disulfide isomerase, PDIp

2 Images

Flow Cytometry (Intracellular) - Anti-PDIA2/PDIP antibody [EPR12212] (AB180931)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PDIA2/PDIP antibody [EPR12212] (AB180931)

Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed)labeling PDIA2/PDIP with ab180931 at 1/100 dilution (green) or Rabbit monoclonal IgG (red), followed with Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Supplier Data

Western blot - Anti-PDIA2/PDIP antibody [EPR12212] (AB180931)

All lanes:

Western blot - Anti-PDIA2/PDIP antibody [EPR12212] (ab180931) at 1/20000 dilution

Lane 1:

Human pancreas lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

BxPC-3 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 58 kDa

false

Carrier free

Anti-PDIA2/PDIP antibody [EPR12212] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12212

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDIA2 also known as PDIP is a protein disulfide isomerase with a prominent role in catalyzing protein folding through the isomerization of disulfide bonds. It is part of the broader family known as PDI proteins. PDIA2 has a mass of approximately 57 kDa and expression occurs mainly in the endoplasmic reticulum. Its enzymatic activity contributes to the maintenance of cellular homeostasis by ensuring proper protein conformation. Researchers have identified PDIA2 as a target for potential PDI inhibitors due to its critical function in protein folding.

Biological function summary

PDIA2 facilitates the correct formation of disulfide bonds which is essential for protein maturation and stability. This protein operates within a multi-component complex involved in oxidative protein folding regulating the redox state in the endoplasmic reticulum environment. By assisting in proper protein assembly PDIA2 maintains the equilibrium of protein synthesis folding and degradation suggesting its importance in cellular protein metabolism.

Pathways

Studies show that PDIA2 plays a role in the oxidative protein folding pathway which is part of the larger cellular response network to oxidative stress. The interaction of PDIA2 with other protein disulfide isomerases such as PDI and ERP57 highlights its role in this critical pathway. Furthermore PDIA2 is linked to the unfolded protein response pathway indicating its involvement in cell stress conditions where misfolded proteins accumulate.

PDIA2 has associations with metabolic disorders and neurodegenerative diseases. Its role in the proper folding of insulin and other secreted proteins links PDIA2 to diabetes-related conditions. Additionally research suggests connections between PDIA2 and neurodegenerative diseases like Alzheimer's where protein misfolding and aggregation play pivotal roles. PDIA2's relationship with proteins like amyloid precursor protein further corroborates its involvement in the pathological processes of such disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.

See full target information PDIA2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 7:5876 PubMed28724915

2017

Quinolinate Phosphoribosyltransferase is an Antiviral Host Factor Against Hepatitis C Virus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Zhilong Wang,Yanhang Gao,Chao Zhang,Haiming Hu,Dongwei Guo,Yi Xu,Qiuping Xu,Weihong Zhang,Sisi Deng,Pingyun Lv,Yan Yang,Yanhua Ding,Qingquan Li,Changjiang Weng,Xinwen Chen,Sitang Gong,Hairong Chen,Junqi Niu,Hong Tang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDIA2/PDIP antibody [EPR12212]

Flow Cytometry (Intracellular) - Anti-PDIA2/PDIP antibody [EPR12212] (AB180931)

Western blot - Anti-PDIA2/PDIP antibody [EPR12212] (AB180931)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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