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AB250264

Anti-PDIA2/PDIP antibody [EPR12212] - BSA and Azide free

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Rabbit Recombinant Monoclonal PDIA2/PDIP antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

PDIP, PDIA2, Protein disulfide-isomerase A2, Pancreas-specific protein disulfide isomerase, PDIp

2 Images
Flow Cytometry (Intracellular) - Anti-PDIA2/PDIP antibody [EPR12212] - BSA and Azide free (AB250264)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PDIA2/PDIP antibody [EPR12212] - BSA and Azide free (AB250264)

This data was developed using ab180931, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling PDIA2/PDIP with ab180931 at 1/100 dilution (green) or Rabbit monoclonal IgG (red), followed with Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Western blot - Anti-PDIA2/PDIP antibody [EPR12212] - BSA and Azide free (AB250264)
  • WB

Supplier Data

Western blot - Anti-PDIA2/PDIP antibody [EPR12212] - BSA and Azide free (AB250264)

This data was developed using ab180931, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PDIA2/PDIP antibody [EPR12212] (<a href='/en-us/products/primary-antibodies/pdia2-pdip-antibody-epr12212-ab180931'>ab180931</a>) at 1/20000 dilution

Lane 1:

Human pancreas lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

BxPC-3 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 58 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12212

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250264 is the carrier-free version of ab180931.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDIA2 also known as PDIP is a protein disulfide isomerase with a prominent role in catalyzing protein folding through the isomerization of disulfide bonds. It is part of the broader family known as PDI proteins. PDIA2 has a mass of approximately 57 kDa and expression occurs mainly in the endoplasmic reticulum. Its enzymatic activity contributes to the maintenance of cellular homeostasis by ensuring proper protein conformation. Researchers have identified PDIA2 as a target for potential PDI inhibitors due to its critical function in protein folding.
Biological function summary

PDIA2 facilitates the correct formation of disulfide bonds which is essential for protein maturation and stability. This protein operates within a multi-component complex involved in oxidative protein folding regulating the redox state in the endoplasmic reticulum environment. By assisting in proper protein assembly PDIA2 maintains the equilibrium of protein synthesis folding and degradation suggesting its importance in cellular protein metabolism.

Pathways

Studies show that PDIA2 plays a role in the oxidative protein folding pathway which is part of the larger cellular response network to oxidative stress. The interaction of PDIA2 with other protein disulfide isomerases such as PDI and ERP57 highlights its role in this critical pathway. Furthermore PDIA2 is linked to the unfolded protein response pathway indicating its involvement in cell stress conditions where misfolded proteins accumulate.

PDIA2 has associations with metabolic disorders and neurodegenerative diseases. Its role in the proper folding of insulin and other secreted proteins links PDIA2 to diabetes-related conditions. Additionally research suggests connections between PDIA2 and neurodegenerative diseases like Alzheimer's where protein misfolding and aggregation play pivotal roles. PDIA2's relationship with proteins like amyloid precursor protein further corroborates its involvement in the pathological processes of such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
See full target information PDIA2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com