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AB327107

Anti-PDIA3 Antibody [CL2446]

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Mouse Monoclonal ERp57 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PDIA3.

View Alternative Names

ERP57, ERP60, GRP58, PDIA3, Protein disulfide-isomerase A3, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, Endoplasmic reticulum resident protein 60, p58, ER protein 57, ERp57, ER protein 60, ERp60

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA3 Antibody [CL2446] (AB327107)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA3 Antibody [CL2446] (AB327107)

Immunohistochemical staining of human liver using ab327107 at 1 : 5000–1 : 10000 dilution, showing moderate cytoplasmic positivity in hepatocytes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA3 Antibody [CL2446] (AB327107)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA3 Antibody [CL2446] (AB327107)

Immunohistochemical staining of human placenta using ab327107 at 1 : 5000–1 : 10000 dilution, showing strong cytoplasmic positivity in trophoblastic cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA3 Antibody [CL2446] (AB327107)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA3 Antibody [CL2446] (AB327107)

Immunohistochemical staining of human small intestine using ab327107 at 1 : 5000–1 : 10000 dilution, showing strong cytoplasmic positivity in glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA3 Antibody [CL2446] (AB327107)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA3 Antibody [CL2446] (AB327107)

Immunohistochemistry analysis in human small intestine and skeletal muscle tissues using ab327107 at 1 : 5000–1 : 10000 dilution. Corresponding PDIA3 RNA-seq data are presented for the same tissues.

Western blot - Anti-PDIA3 Antibody [CL2446] (AB327107)
  • WB

Supplier Data

Western blot - Anti-PDIA3 Antibody [CL2446] (AB327107)

Western blot analysis using ab327107 at 1 µg/ml.
Lane 1 : Marker [kDa]
Lane 2 : Human cell line U-251 MG

All lanes:

Western blot - Anti-PDIA3 Antibody [CL2446] (ab327107)

All lanes:

Human cell line U-251 MG

Predicted band size: 56.78 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA3 Antibody [CL2446] (AB327107)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA3 Antibody [CL2446] (AB327107)

Immunohistochemical staining of human skeletal muscle using ab327107 at 1 : 5000–1 : 10000 dilution, showing no positivity in myocytes as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2446

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PDIA3.

P30101

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/10000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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Properties and storage information

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone (PubMed : 11825568, PubMed : 16193070, PubMed : 27897272, PubMed : 36104323, PubMed : 7487104). Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it functions as an essential folding chaperone for TAPBP. Through TAPBP, assists the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Therefore, plays a crucial role in the presentation of antigens to cytotoxic T cells in adaptive immunity (PubMed : 35948544, PubMed : 36104323).
See full target information PDIA3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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