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AB232979

Anti-PDIA5 antibody

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(1 Publication)

Rabbit Polyclonal PDIA5 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PDIA5 aa 350 to C-terminus.

View Alternative Names

PDIR, PDIA5, Protein disulfide-isomerase A5, Protein disulfide isomerase-related protein

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA5 antibody (AB232979)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA5 antibody (AB232979)

Formalin-fixed, paraffin-embedded human liver tissue stained for PDIA5 using ab232979 at 30 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-PDIA5 antibody (AB232979)
  • WB

Supplier Data

Western blot - Anti-PDIA5 antibody (AB232979)

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Western blot - Anti-PDIA5 antibody (ab232979) at 2 µg/mL

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HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 60 kDa

false

Western blot - Anti-PDIA5 antibody (AB232979)
  • WB

Supplier Data

Western blot - Anti-PDIA5 antibody (AB232979)

All lanes:

Western blot - Anti-PDIA5 antibody (ab232979) at 2 µg/mL

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A431 (human epidermoid carcinoma cell line) cell lysate

Predicted band size: 60 kDa

false

Western blot - Anti-PDIA5 antibody (AB232979)
  • WB

Supplier Data

Western blot - Anti-PDIA5 antibody (AB232979)

All lanes:

Western blot - Anti-PDIA5 antibody (ab232979) at 2 µg/mL

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Pig liver lysate

Predicted band size: 60 kDa

false

Western blot - Anti-PDIA5 antibody (AB232979)
  • WB

Supplier Data

Western blot - Anti-PDIA5 antibody (AB232979)

All lanes:

Western blot - Anti-PDIA5 antibody (ab232979) at 2 µg/mL

All lanes:

Recombinant human PDIA5 protein

Predicted band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PDIA5 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14554

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein disulfide-isomerase A5 (PDIA5) also known as PDIR serves as an enzyme that catalyzes the formation breakage and rearrangement of disulfide bonds. It weighs approximately 58 kDa. Researchers find PDIA5 expressed in the endoplasmic reticulum especially in tissues with high secretory activity like the pancreas and liver. It participates in protein folding and maintaining cell function by ensuring proper protein structure through disulfide bond formation.
Biological function summary

PDIA5 has important roles in regulating redox homeostasis and protein quality control within the endoplasmic reticulum. It does not operate alone; it functions as part of multi-protein complexes engaging with other chaperones to assist in folding newly synthesized proteins. These interactions prevent protein aggregation and accumulation of misfolded proteins protecting cells from stress.

Pathways

PDIA5 integrates into the unfolded protein response (UPR) and endoplasmic reticulum-associated degradation (ERAD) pathways. It interfaces with other proteins like BiP/GRP78 which enhances its chaperone activity during cellular stress and EDEM1 a protein involved in recognizing misfolded glycoproteins. These pathways work together to maintain cellular homeostasis by managing protein load and degradation processes.

Research associates PDIA5 with various conditions including neurodegenerative diseases and cancer. Misfolding and aggregation of proteins linked to PDIA5 malfunction can contribute to disorders like Alzheimer's disease. Moreover PDIA5 may influence cancer progression by interacting with proteins like CALR which also participates in cell stress responses. Understanding PDIA5 functions helps in developing potential therapeutic strategies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information PDIA5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 35:102380 PubMed39640012

2024

A lipid nanoparticle-based oligodendrocyte-specific mRNA therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Masanori Sawamura,Kiyoshi Tachikawa,Rie Hikawa,Hisako Akiyama,Seiji Kaji,Ken Yasuda,Angel I Leu,Hyojung Hong,Rajesh Mukthavaram,Pad Chivukula,Hodaka Yamakado,Yoshio Hirabayashi,Ryosuke Takahashi,Shu-Ichi Matsuzawa
View all publications
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Product promise

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