Rabbit Polyclonal PDIA6 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human PDIA6 aa 250-300.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human PDIA6 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q15084
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
May function as a chaperone that inhibits aggregation of misfolded proteins (PubMed:12204115). Negatively regulates the unfolded protein response (UPR) through binding to UPR sensors such as ERN1, which in turn inactivates ERN1 signaling (PubMed:24508390). May also regulate the UPR via the EIF2AK3 UPR sensor (PubMed:24508390). Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin (PubMed:15466936).
Alternative names
Protein disulfide-isomerase A6, Endoplasmic reticulum protein 5, Protein disulfide isomerase P5, Thioredoxin domain-containing protein 7, ER protein 5, ERp5, TXNDC7, P5, ERP5, PDIA6
Recommended products
Rabbit Polyclonal PDIA6 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human PDIA6 aa 250-300.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human PDIA6 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q15084
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab226840 was affinity purified using an epitope specific to PDIA6 immobilized on solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDIA6 also known as Protein Disulfide Isomerase Family A Member 6 is a protein with a molecular mass of approximately 48 kDa. This protein resides primarily in the endoplasmic reticulum (ER) where it engages in the formation and rearrangement of disulfide bonds during the protein folding process. PDIA6 also facilitates chaperone activity aiding the proper folding of nascent proteins. Its expression is widespread but shows higher levels in secretory tissues reflecting its involvement in protein synthesis and processing.
- Biological function summary
PDIA6 plays a critical role in maintaining cellular homeostasis by ensuring correct protein folding within the ER. It interacts with various substrates and is part of a larger protein complex along with other ER-resident chaperones and foldases. This protein also responds to ER stress by assisting in the unfolded protein response (UPR) a signaling cascade that restores normal function of the ER or triggers apoptosis if the stress is unresolved.
- Pathways
PDIA6 holds a significant position in the folding modification and degradation of proteins via the ER-associated degradation (ERAD) pathway. This pathway involves collaboration with proteins such as BiP and ERp72 which together prevent accumulation of misfolded proteins. PDIA6 also contributes to the cellular redox homeostasis playing an important role in maintaining the balance of oxidative states within the cell which can influence pathways like the UPR.
- Associated diseases and disorders
PDIA6 associates with conditions characterized by ER stress including neurodegenerative disorders like Alzheimer's disease as well as cancer. In Alzheimer’s disease alterations in its function may affect the handling of misfolded proteins aligning it with amyloid precursor protein processing. In cancer its modulation of protein folding and stress responses links it to oncogenic pathways interacting with proteins such as p53 which can influence cell apoptosis and survival.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Western blot - Anti-PDIA6 antibody (ab226840)
Lysates were prepared using NETN lysis buffer.
All lanes: Western blot - Anti-PDIA6 antibody (ab226840) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4: TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg
Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 48 kDa
Exposure time: 30s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA6 antibody (ab226840)
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PDIA6 using ab226840 at 1/5000 dilution in immunohistochemical analysis.
Detection: DAB staining. Counterstain: Hematoxylin (blue).
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