JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB227545

Anti-PDIA6 antibody

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Polyclonal PDIA6 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PDIA6.

View Alternative Names

ERP5, P5, TXNDC7, PDIA6, Protein disulfide-isomerase A6, Endoplasmic reticulum protein 5, Protein disulfide isomerase P5, Thioredoxin domain-containing protein 7, ER protein 5, ERp5

4 Images
Immunoprecipitation - Anti-PDIA6 antibody (AB227545)
  • IP

Supplier Data

Immunoprecipitation - Anti-PDIA6 antibody (AB227545)

PDIA6 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 μg ab227545. Western blot was performed from the immunoprecipitate using ab227545.

Lane 1 : HEK-293T whole cell extract (Input).

Lane 2 : Control IgG IP in HEK-293T whole cell extract.

Lane 3 : ab227545 IP in HEK-293T whole cell extract.

All lanes:

Immunoprecipitation - Anti-PDIA6 antibody (ab227545)

Predicted band size: 48 kDa

true

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA6 antibody (AB227545)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA6 antibody (AB227545)

Paraffin-embedded rat liver tissue stained for PDIA6 with ab227545 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA6 antibody (AB227545)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDIA6 antibody (AB227545)

Paraffin-embedded mouse liver tissue stained for PDIA6 with ab227545 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-PDIA6 antibody (AB227545)
  • WB

Supplier Data

Western blot - Anti-PDIA6 antibody (AB227545)

10% SDS-PAGE

All lanes:

Western blot - Anti-PDIA6 antibody (ab227545) at 1/1000 dilution

All lanes:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

Predicted band size: 48 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

IP, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PDIA6. The exact immunogen used to generate this antibody is proprietary information.

Q15084

style
left-column

Alternative Products

Primary Antibodies

AB11432

Anti-PDIA6 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PDIA6 antibody (AB11432)

  • 0
  • 0
Primary Antibodies

AB201733

HRP Anti-PDIA6 antibody [EPR10132(B)]

RabMAb|Recombinant

Western blot - HRP Anti-PDIA6 antibody [EPR10132(B)] (AB201733)

  • 0
  • 0
Primary Antibodies

AB154820

Anti-PDIA6 antibody [EPR10132(B)]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PDIA6 antibody [EPR10132(B)] (AB154820)

  • 5
  • 3

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB21679

Anti-Clathrin heavy chain antibody

Western blot - Anti-Clathrin heavy chain antibody (AB21679)

  • 5
  • 38
Primary Antibodies

AB137110

Anti-P4HB antibody [EPR9499]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-P4HB antibody [EPR9499] (AB137110)

  • 4
  • 6
Primary Antibodies

AB22595

Anti-Calnexin antibody - ER Marker

Lab Essentials|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - ER Marker (AB22595)

  • 5
  • 28
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDIA6 also known as Protein Disulfide Isomerase Family A Member 6 is a protein with a molecular mass of approximately 48 kDa. This protein resides primarily in the endoplasmic reticulum (ER) where it engages in the formation and rearrangement of disulfide bonds during the protein folding process. PDIA6 also facilitates chaperone activity aiding the proper folding of nascent proteins. Its expression is widespread but shows higher levels in secretory tissues reflecting its involvement in protein synthesis and processing.
Biological function summary

PDIA6 plays a critical role in maintaining cellular homeostasis by ensuring correct protein folding within the ER. It interacts with various substrates and is part of a larger protein complex along with other ER-resident chaperones and foldases. This protein also responds to ER stress by assisting in the unfolded protein response (UPR) a signaling cascade that restores normal function of the ER or triggers apoptosis if the stress is unresolved.

Pathways

PDIA6 holds a significant position in the folding modification and degradation of proteins via the ER-associated degradation (ERAD) pathway. This pathway involves collaboration with proteins such as BiP and ERp72 which together prevent accumulation of misfolded proteins. PDIA6 also contributes to the cellular redox homeostasis playing an important role in maintaining the balance of oxidative states within the cell which can influence pathways like the UPR.

PDIA6 associates with conditions characterized by ER stress including neurodegenerative disorders like Alzheimer's disease as well as cancer. In Alzheimer’s disease alterations in its function may affect the handling of misfolded proteins aligning it with amyloid precursor protein processing. In cancer its modulation of protein folding and stress responses links it to oncogenic pathways interacting with proteins such as p53 which can influence cell apoptosis and survival.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

May function as a chaperone that inhibits aggregation of misfolded proteins (PubMed : 12204115). Negatively regulates the unfolded protein response (UPR) through binding to UPR sensors such as ERN1, which in turn inactivates ERN1 signaling (PubMed : 24508390). May also regulate the UPR via the EIF2AK3 UPR sensor (PubMed : 24508390). Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin (PubMed : 15466936).
See full target information PDIA6
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Chinese medical journal 136:1177-1187 PubMed37083129

2023

Single-cell RNA sequencing reveals the transcriptomic landscape of kidneys in patients with ischemic acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Tang,Peng Jin,Chanjuan Shen,Wei Lin,Leilin Yu,Xueling Hu,Ting Meng,Linlin Zhang,Ling Peng,Xiangcheng Xiao,Peter Eggenhuizen,Joshua D Ooi,Xueqin Wu,Xiang Ding,Yong Zhong

Experimental and therapeutic medicine 25:136 PubMed36845952

2023

FNDC5 and AKR1B10 inhibit the proliferation and metastasis of adrenocortical carcinoma cells by regulating AMPK/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Danyan Chen,Rongxi Huang,Fang Ren,Hongman Wang,Chengjian Wang,Yu Zhang

Experimental and therapeutic medicine 22:947 PubMed34335889

2021

Long non-coding RNA PCAT6 regulates bladder cancer progression via the microRNA-143-3p/PDIA6 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanjie Zhang,Lin Chen,Gang Luo

Molecular and cellular biochemistry 476:1751-1763 PubMed33433832

2021

The circular RNA circSlc7a11 promotes bone cancer pain pathogenesis in rats by modulating LLC-WRC 256 cell proliferation and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Wen Chen,Xiao-Xia Zhang,Zhu-Ding Peng,Zu-Min Xing,Yi-Wen Zhang,Ya-Lan Li
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com