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AB191851

Anti-PDILT antibody

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Rabbit Polyclonal PDILT antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Mouse samples. Immunogen corresponding to Synthetic Peptide within Mouse Pdilt aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Protein disulfide-isomerase-like protein of the testis, Pdilt

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDILT antibody (AB191851)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDILT antibody (AB191851)

Immunohistochemical staining of paraffin embedded mouse testis tissue using ab191851 at a dilution of 1/1000. The secondary antibody was used at a dilution of 1/1000 and the sample was counter stained with DAPI.

Immunocytochemistry/ Immunofluorescence - Anti-PDILT antibody (AB191851)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PDILT antibody (AB191851)

Immunofluorescent analysis of paraformaldehyde-fixed, cryopreserved in graded 10-30% sucrose, and embedded in a TissueTek OCT compound mouse testicular sections labeling PDILT with ab191851 at 1/100 and as a secondary antibody, Alexa Fluor 546 conjugated anti-rabbit IgG antibody was used. Image at the top : mouse spermatocyte; image at the bottom mouse spermatid.

Immunoprecipitation - Anti-PDILT antibody (AB191851)
  • IP

Supplier Data

Immunoprecipitation - Anti-PDILT antibody (AB191851)

Immunoprecipitation of PDILT protein from mouse testis extracts. Extracts (100 μg) of wild-type (+/+) and PDILT (-/-) knock out mouse testes were immunoprecipitated with ab191851 at 1/100 dilution and the precipitates were analyzed by western blotting using ab191851 at 1/1000 dilution.

1. Input. 2. Wild-type. 3. PDILT (-/-) knock out

All lanes:

Immunoprecipitation - Anti-PDILT antibody (ab191851)

Predicted band size: 67 kDa

false

Western blot - Anti-PDILT antibody (AB191851)
  • WB

Supplier Data

Western blot - Anti-PDILT antibody (AB191851)

All lanes:

Western blot - Anti-PDILT antibody (ab191851) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 10 µg

Lane 2:

Mouse sperm cell lysate at 10 µg

Secondary

All lanes:

HRP anti-rabbit IgG at 1/10000 dilution

Predicted band size: 67 kDa

false

Western blot - Anti-PDILT antibody (AB191851)
  • WB

Supplier Data

Western blot - Anti-PDILT antibody (AB191851)

Lower panel : detection of alpha tubulin was used as loading control.

All lanes:

Western blot - Anti-PDILT antibody (ab191851) at 1/1000 dilution

Lane 1:

Testes extracts from wild-type (+/+) mice

Lane 2:

Testes extracts from PDILT single knock-out mice (+/-)

Lane 3:

Testes extracts from PDILT double knock-out mice (-/-)

Predicted band size: 67 kDa

false

Western blot - Anti-PDILT antibody (AB191851)
  • WB

Supplier Data

Western blot - Anti-PDILT antibody (AB191851)

All lanes:

Western blot - Anti-PDILT antibody (ab191851) at 1/1000 dilution

Lane 1:

mouse brain tissue lysate at 30 µg

Lane 2:

mouse lung tissue lysate at 30 µg

Lane 3:

mouse heart tissue lysate at 30 µg

Lane 4:

mouse thymus tissue lysate at 30 µg

Lane 5:

mouse liver tissue lysate at 30 µg

Lane 6:

mouse spleen tissue lysate at 30 µg

Lane 7:

mouse kidney tissue lysate at 30 µg

Lane 8:

mouse muscle tissue lysate at 30 µg

Lane 9:

mouse ovary tissue lysate at 30 µg

Lane 10:

mouse testis tissue lysate at 30 µg

Lane 11:

mouse sperm lysate at 10 µg

Predicted band size: 67 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P, WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Mouse Pdilt aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin. Database link Q9DAN1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
pH: 6 - 8.5 Preservative: 0.1% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDILT also known as Protein Disulfide Isomerase-Like Testis Expressed is a member of the protein disulfide isomerase (PDI) family. This protein has a molecular mass of approximately 70 kDa and a unique structure. PDILT lacks one of the two active CGHC motifs usually seen in this family while it possesses the ER-retention signal KDEL. This protein is expressed mainly in the testis with a strong association to male germ cells. Its expression links to the endoplasmic reticulum suggesting a role in protein folding and quality control.
Biological function summary

PDILT plays an essential role in ensuring proper sperm development. Although it is not formally part of a larger protein complex it interacts with other proteins in the testis to facilitate the assembly of protein disulfide bonds. These interactions ensure the correct formation of complex disulfide-bonded proteins which are necessary for sperm structure and function. PDILT is involved in managing oxidative stress within the cells during the process of spermatogenesis.

Pathways

PDILT plays an important part in the oxidative protein folding pathway within the endoplasmic reticulum. This pathway is critical for forming disulfide bonds in proteins a process important for their stability and function. PDILT has connections with proteins like Calnexin an important component of the quality control pathway in the ER. Its functional synergy with other PDI family members highlights its role in maintaining the balance of protein homeostasis during reproduction.

PDILT has shown links to male infertility particularly regarding improper sperm motility or structure. Its malfunction can lead to a disruption in the formation of sperm disulfide bonds resulting in structural abnormalities. This dysfunction associates with other proteins like Calreticulin which is also involved in proper protein folding. Current research continues to investigate PDILT's broader implications in fertility emphasizing its significance in maintaining reproductive health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable redox-inactive chaperone involved in spermatogenesis.
See full target information Pdilt
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Product promise

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