Rabbit Polyclonal PDILT antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Mouse samples. Immunogen corresponding to Synthetic Peptide within Mouse Pdilt aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Pdilt aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9DAN1
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Mouse | Tested | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Target data
Function
Probable redox-inactive chaperone involved in spermatogenesis.
Alternative names
Protein disulfide-isomerase-like protein of the testis, Pdilt
Recommended products
Rabbit Polyclonal PDILT antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Mouse samples. Immunogen corresponding to Synthetic Peptide within Mouse Pdilt aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
- Immunogens
- Synthetic Peptide within Mouse Pdilt aa 550 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9DAN1
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDILT also known as Protein Disulfide Isomerase-Like Testis Expressed is a member of the protein disulfide isomerase (PDI) family. This protein has a molecular mass of approximately 70 kDa and a unique structure. PDILT lacks one of the two active CGHC motifs usually seen in this family while it possesses the ER-retention signal KDEL. This protein is expressed mainly in the testis with a strong association to male germ cells. Its expression links to the endoplasmic reticulum suggesting a role in protein folding and quality control.
- Biological function summary
PDILT plays an essential role in ensuring proper sperm development. Although it is not formally part of a larger protein complex it interacts with other proteins in the testis to facilitate the assembly of protein disulfide bonds. These interactions ensure the correct formation of complex disulfide-bonded proteins which are necessary for sperm structure and function. PDILT is involved in managing oxidative stress within the cells during the process of spermatogenesis.
- Pathways
PDILT plays an important part in the oxidative protein folding pathway within the endoplasmic reticulum. This pathway is critical for forming disulfide bonds in proteins a process important for their stability and function. PDILT has connections with proteins like Calnexin an important component of the quality control pathway in the ER. Its functional synergy with other PDI family members highlights its role in maintaining the balance of protein homeostasis during reproduction.
- Associated diseases and disorders
PDILT has shown links to male infertility particularly regarding improper sperm motility or structure. Its malfunction can lead to a disruption in the formation of sperm disulfide bonds resulting in structural abnormalities. This dysfunction associates with other proteins like Calreticulin which is also involved in proper protein folding. Current research continues to investigate PDILT's broader implications in fertility emphasizing its significance in maintaining reproductive health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDILT antibody (ab191851)
Immunohistochemical staining of paraffin embedded mouse testis tissue using ab191851 at a dilution of 1/1000. The secondary antibody was used at a dilution of 1/1000 and the sample was counter stained with DAPI.
Western blot - Anti-PDILT antibody (ab191851)
All lanes: Western blot - Anti-PDILT antibody (ab191851) at 1/1000 dilution
Lane 1: Mouse testis tissue lysate at 10 µg
Lane 2: Mouse sperm cell lysate at 10 µg
SecondaryAll lanes: HRP anti-rabbit IgG at 1/10000 dilution
Predicted band size: 67 kDa
Western blot - Anti-PDILT antibody (ab191851)
All lanes: Western blot - Anti-PDILT antibody (ab191851) at 1/1000 dilution
Lane 1: mouse brain tissue lysate at 30 µg
Lane 2: mouse lung tissue lysate at 30 µg
Lane 3: mouse heart tissue lysate at 30 µg
Lane 4: mouse thymus tissue lysate at 30 µg
Lane 5: mouse liver tissue lysate at 30 µg
Lane 6: mouse spleen tissue lysate at 30 µg
Lane 7: mouse kidney tissue lysate at 30 µg
Lane 8: mouse muscle tissue lysate at 30 µg
Lane 9: mouse ovary tissue lysate at 30 µg
Lane 10: mouse testis tissue lysate at 30 µg
Lane 11: mouse sperm lysate at 10 µg
Predicted band size: 67 kDa
Immunoprecipitation - Anti-PDILT antibody (ab191851)
Immunoprecipitation of PDILT protein from mouse testis extracts. Extracts (100 μg) of wild-type (+/+) and PDILT (-/-) knock out mouse testes were immunoprecipitated with ab191851 at 1/100 dilution and the precipitates were analyzed by western blotting using ab191851 at 1/1000 dilution.
1. Input. 2. Wild-type. 3. PDILT (-/-) knock out
All lanes: Immunoprecipitation - Anti-PDILT antibody (ab191851)
Predicted band size: 67 kDa
Western blot - Anti-PDILT antibody (ab191851)
Lower panel: detection of alpha tubulin was used as loading control.
All lanes: Western blot - Anti-PDILT antibody (ab191851) at 1/1000 dilution
Lane 1: Testes extracts from wild-type (+/+) mice
Lane 2: Testes extracts from PDILT single knock-out mice (+/-)
Lane 3: Testes extracts from PDILT double knock-out mice (-/-)
Predicted band size: 67 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PDILT antibody (ab191851)
Immunofluorescent analysis of paraformaldehyde-fixed, cryopreserved in graded 10-30% sucrose, and embedded in a TissueTek OCT compound mouse testicular sections labeling PDILT with ab191851 at 1/100 and as a secondary antibody, Alexa Fluor 546 conjugated anti-rabbit IgG antibody was used. Image at the top: mouse spermatocyte; image at the bottom mouse spermatid.
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