AB90444

Anti-PDK1 antibody

Name: Anti-PDK1 antibody (ab90444) | Abcam
Brand: Abcam Limited
SKU: AB90444
Rating: 4 (1 reviews)

(1 Review)

(11 Publications)

Rabbit Polyclonal PDK1 antibody. Suitable for WB and reacts with Human, Pig, Mouse, Rat, Rabbit, African green monkey, Dog samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human PDK1 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1

1 Images

Unknown

Western blot - Anti-PDK1 antibody (AB90444)

All lanes:

Western blot - Anti-PDK1 antibody (ab90444) at 1/1000 dilution

Lane 1:

HeLa cell lysate

Lane 2:

Rat brain lysate

Lane 3:

Mouse brain lysate

Lane 4:

Vero cell lysate

Lane 5:

ESK-4 cell lysate

Lane 6:

RK-13 cell lysate

Lane 7:

MDCK cell lysate

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Dog, Human, Pig, Rabbit, African green monkey

Applications

applications

Immunogen

Synthetic Peptide within Human PDK1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q15118

style

left-column

Alternative Products

Primary Antibodies

AB207450

Anti-PDK1 antibody [EPR19573]

RabMAb|Recombinant|KO Validated

Western blot - Anti-PDK1 antibody [EPR19573] (AB207450)

Primary Antibodies

AB110025

Anti-PDK1 antibody [4A11]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [4A11] (AB110025)

Primary Antibodies

AB227682

Anti-PDK1 antibody [SP270] - C-terminal

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - C-terminal (AB227682)

Primary Antibodies

AB180171

Anti-PDK1 antibody [EPR13015] - C-terminal

RabMAb|Recombinant|KO Validated

Western blot - Anti-PDK1 antibody [EPR13015] - C-terminal (AB180171)

Primary Antibodies

AB175190

Anti-PDK1 antibody [EPR13009]

RabMAb|Recombinant|KO Validated

Western blot - Anti-PDK1 antibody [EPR13009] (AB175190)

Primary Antibodies

AB110335

Anti-PDK1 antibody [2H3AA11]

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [2H3AA11] (AB110335)

Primary Antibodies

AB202468

Anti-PDK1 antibody [EPR19571]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [EPR19571] (AB202468)

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Primary Antibodies

AB209847

Anti-Hexokinase II antibody [EPR20839]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-Hexokinase II antibody [EPR20839] (AB209847)

Primary Antibodies

AB115730

Anti-Glucose Transporter GLUT1 antibody [EPR3915]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB115730)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2 (PubMed : 7499431, PubMed : 18541534, PubMed : 22195962, PubMed : 26942675, PubMed : 17683942). This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate (PubMed : 18541534, PubMed : 22195962, PubMed : 26942675). Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia (PubMed : 18541534, PubMed : 22195962, PubMed : 26942675).

See full target information PDK1

style

left-column

Publications (11)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 299:102748 PubMed36436564

2022

Glucose transporter GLUT1 expression is important for oriental river prawn (Macrobrachium nipponense) hemocyte adaptation to hypoxic conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Xichao Sun,Cheng Xue,Yiting Jin,Chao Bian,Na Zhou,Shengming Sun

Journal of molecular and cellular cardiology 164:136-147 PubMed34923199

2021

A roadmap for the characterization of energy metabolism in human cardiomyocytes derived from induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Emanuelli,Anna Zoccarato,Christina M Reumiller,Angelos Papadopoulos,Mei Chong,Sabine Rebs,Kai Betteridge,Matteo Beretta,Katrin Streckfuss-Bömeke,Ajay M Shah

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 29:785-792 PubMed32750756

2020

PD-L1, PDK-1 and p-Akt are correlated in patients with papillary thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Wang,Zhengdong Zhang,Zhe Yan,Shihong Ma

Cells 9: PubMed32197467

2020

Elevated PDK1 Expression Drives PI3K/AKT/MTOR Signaling Promotes Radiation-Resistant and Dedifferentiated Phenotype of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Oluwaseun Adebayo Bamodu,Hang-Lung Chang,Jiann-Ruey Ong,Wei-Hwa Lee,Chi-Tai Yeh,Jo-Ting Tsai

American journal of physiology. Regulatory, integr 317:R513-R520 PubMed31314546

2019

Increased pyruvate dehydrogenase activity in skeletal muscle of growth-restricted ovine fetuses.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander L Pendleton,Laurel R Humphreys,Melissa A Davis,Leticia E Camacho,Miranda J Anderson,Sean W Limesand

The Journal of biological chemistry 294:7810-7820 PubMed30923124

2019

Lactic acidosis caused by repressed lactate dehydrogenase subunit B expression down-regulates mitochondrial oxidative phosphorylation via the pyruvate dehydrogenase (PDH)-PDH kinase axis.

Applications

Unspecified application

Species

Unspecified reactive species

Sun Mi Hong,Young-Kyoung Lee,Imkyong Park,So Mee Kwon,Seongki Min,Gyesoon Yoon

Pflugers Archiv : European journal of physiology 470:1597-1613 PubMed30069669

2018

Impact of skeletal muscle IL-6 on regulation of liver and adipose tissue metabolism during fasting.

Applications

Unspecified application

Species

Unspecified reactive species

Lærke Bertholdt,Anders Gudiksen,Henrik Jessen,Henriette Pilegaard

Pflugers Archiv : European journal of physiology 470:1633-1645 PubMed29946822

2018

Training state and fasting-induced PDH regulation in human skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Anders Gudiksen,Lærke Bertholdt,Tomasz Stankiewicz,Ida Villesen,Jens Bangsbo,Peter Plomgaard,Henriette Pilegaard

Neuro-oncology 20:343-354 PubMed29016888

2017

Metabolic heterogeneity and plasticity of glioma stem cells in a mouse glioblastoma model.

Applications

Unspecified application

Species

Unspecified reactive species

Shunsuke Shibao,Noriaki Minami,Naoyoshi Koike,Nobuyuki Fukui,Kazunari Yoshida,Hideyuki Saya,Oltea Sampetrean

American journal of physiology. Endocrinology and metabolism 312:E204-E214 PubMed28028037

2016

Muscle interleukin-6 and fasting-induced PDH regulation in mouse skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Anders Gudiksen,Laerke Bertholdt,Mikkel Birkkjaer Vingborg,Henriette Watson Hansen,Stine Ringholm,Henriette Pilegaard

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com