Rabbit Recombinant Monoclonal PDK1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication.
Images
![Western blot - Anti-PDK1 antibody [EPR13009] (AB175190), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr13009-ab175190--western-blot-img57875.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PDK1 antibody [EPR13009] (AB175190)")
![Western blot - Anti-PDK1 antibody [EPR13009] (AB175190), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr13009-ab175190--western-blot-img136821.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PDK1 antibody [EPR13009] (AB175190)")
![Immunoprecipitation - Anti-PDK1 antibody [EPR13009] (AB175190), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr13009-ab175190--immunoprecipitation-img73094.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PDK1 antibody [EPR13009] (AB175190)")
![Western blot - Anti-PDK1 antibody [EPR13009] (AB175190), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr13009-ab175190--western-blot-img146213.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PDK1 antibody [EPR13009] (AB175190)")
Publications
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- Osteoarthritis and cartilage 24:1113-242016Hypoxia regulates sumoylation pathways in intervertebral disc cells: implications for hypoxic adaptations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesF Wang et. al.PubMed 26826302
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Expected | Not recommended | Expected | Not recommended | Not recommended |
| Rat | Predicted | Not recommended | Predicted | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10 - 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
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7 products for Alternative Product
Target data
Function
Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
Alternative names
PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1
Recommended products
Rabbit Recombinant Monoclonal PDK1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR13009
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-PDK1 antibody [EPR13009] (ab175190), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr13009-ab175190--western-blot-img57875.jpg "Western blot - Anti-PDK1 antibody [EPR13009] (ab175190)")
Western blot - Anti-PDK1 antibody [EPR13009] (ab175190)
Lanes 1- 2: Merged signal (red and green). Green - ab175190 observed at 46 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.ab175190 was shown to react with PDK1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264791 (knockout cell lysate ab257164) was used. Wild-type HeLa and PDK1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab175190 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PDK1 antibody [EPR13009] (ab175190) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: PDK1 knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 46 kDa
![Western blot - Anti-PDK1 antibody [EPR13009] (ab175190), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr13009-ab175190--western-blot-img136821.jpg "Western blot - Anti-PDK1 antibody [EPR13009] (ab175190)")
Western blot - Anti-PDK1 antibody [EPR13009] (ab175190)
Lanes 1 - 4: Merged signal (red and green). Green - ab175190 observed at 46 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab175190 was shown to specifically recognize PDK1 in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when PDK1 knockout samples were examined. Wild-type and PDK1 knockout samples were subjected to SDS-PAGE. ab175190 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-PDK1 antibody [EPR13009] (ab175190) at 1/1000 dilution
Lane 1: Wild-type HAP1 cell lysate at 20 µg
Lane 2: PDK1 knockout HAP1 cell lysate at 20 µg
Lane 3: Human fetal heart lysate at 20 µg
Lane 4: LnCaP cell lysate at 20 µg
Predicted band size: 49 kDa
![Immunoprecipitation - Anti-PDK1 antibody [EPR13009] (ab175190), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr13009-ab175190--immunoprecipitation-img73094.jpg "Immunoprecipitation - Anti-PDK1 antibody [EPR13009] (ab175190)")
Immunoprecipitation - Anti-PDK1 antibody [EPR13009] (ab175190)
Western blot analysis on immunoprecipitation pellet from Human fetal heart lysate (lane 1) labeling PDK1 with ab175190 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.
All lanes: Immunoprecipitation - Anti-PDK1 antibody [EPR13009] (ab175190)
Predicted band size: 49 kDa
![Western blot - Anti-PDK1 antibody [EPR13009] (ab175190), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr13009-ab175190--western-blot-img146213.jpg "Western blot - Anti-PDK1 antibody [EPR13009] (ab175190)")
Western blot - Anti-PDK1 antibody [EPR13009] (ab175190)
All lanes: Western blot - Anti-PDK1 antibody [EPR13009] (ab175190) at 1/1000 dilution
Lane 1: LNCaP lysates at 10 µg
Lane 2: Human fetal heart lysates at 10 µg
Predicted band size: 49 kDa
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