Rabbit Recombinant Monoclonal PDK1 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-PDK1 antibody [EPR13015] - BSA and Azide free (AB250175), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr13015-bsa-and-azide-free-ab250175--western-blot-img88754.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PDK1 antibody [EPR13015] - BSA and Azide free (AB250175)")
![Western blot - Anti-PDK1 antibody [EPR13015] - BSA and Azide free (AB250175), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr13015-bsa-and-azide-free-ab250175--western-blot-img142294.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PDK1 antibody [EPR13015] - BSA and Azide free (AB250175)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | IP | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Predicted | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Predicted | Not recommended | Not recommended | Not recommended |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
Alternative names
PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1
Recommended products
Rabbit Recombinant Monoclonal PDK1 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR13015
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250175 is the carrier-free version of Anti-PDK1 antibody [EPR13015] - C-terminal ab180171.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr13015-bsa-and-azide-free-ab250175--western-blot-img88754.jpg "Western blot - Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175)")
Western blot - Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175)
This data was developed using Anti-PDK1 antibody [EPR13015] - C-terminal ab180171, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Mitochondrial Pyruvate dehydrogenase kinase 1 knockout HAP1 cell lysate (20 μg)
Lane 3: Human fetal heart tissue lysate (20 μg)
Lane 4: LnCaP cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-PDK1 antibody [EPR13015] - C-terminal ab180171 observed at 46 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-PDK1 antibody [EPR13015] - C-terminal ab180171 was shown to specifically react with Mitochondrial Pyruvate dehydrogenase kinase 1 in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when Mitochondrial Pyruvate dehydrogenase kinase 1 knockout samples were examined. Wild-type and Mitochondrial Pyruvate dehydrogenase kinase 1 knockout samples were subjected to SDS-PAGE. Anti-PDK1 antibody [EPR13015] - C-terminal ab180171 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PDK1 antibody [EPR13015] - C-terminal (Anti-PDK1 antibody [EPR13015] - C-terminal ab180171)
Predicted band size: 49 kDa
![Western blot - Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr13015-bsa-and-azide-free-ab250175--western-blot-img142294.jpg "Western blot - Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175)")
Western blot - Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175)
This data was developed using Anti-PDK1 antibody [EPR13015] - C-terminal ab180171, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-PDK1 antibody [EPR13015] - C-terminal (Anti-PDK1 antibody [EPR13015] - C-terminal ab180171) at 1/10000 dilution
Lane 1: Fetal heart lysate at 10 µg
Lane 2: LNCaP cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
SecondaryAll lanes: Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
Downloads
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