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Rabbit Recombinant Monoclonal PDK1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 24 publications.

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Images

Western blot - Anti-PDK1 antibody [EPR19571] (AB202468), expandable thumbnail
  • Immunoprecipitation - Anti-PDK1 antibody [EPR19571] (AB202468), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [EPR19571] (AB202468), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PDK1 antibody [EPR19571] (AB202468), expandable thumbnail
  • Western blot - Anti-PDK1 antibody [EPR19571] (AB202468), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Expected
Tested
Expected
Expected
Mouse
Tested
Tested
Tested
Tested
Rat
Expected
Tested
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/40
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

-

Species
Rat
Dilution info
1/2000
Notes

-

Species
Human
Dilution info
1/2000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/600
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

Alternative names

PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1

Recommended products

Rabbit Recombinant Monoclonal PDK1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 24 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19571
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-PDK1 antibody [EPR19571] (ab202468), expandable thumbnail

    Western blot - Anti-PDK1 antibody [EPR19571] (ab202468)

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
    Lane 2: PDK1 knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HeLa whole cell lysate (20 μg)
    Lane 4: Jurkat whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab202468 observed at 45 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab202468 was shown to specifically react with PDK1 in wild-type cells along with additional cross reactive bands. Signal was lost in PDK1 knockout cells. Wild-type and PDK1 knockout samples were subjected to SDS-PAGE. ab202468 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-PDK1 antibody [EPR19571] (ab202468)

    Predicted band size: 49 kDa

  • Immunoprecipitation - Anti-PDK1 antibody [EPR19571] (ab202468), expandable thumbnail

    Immunoprecipitation - Anti-PDK1 antibody [EPR19571] (ab202468)

    PDK1 was immunoprecipitated from 1mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab202468 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab202468 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: NIH/3T3 whole cell lysate, 10μg (Input).

    Lane 2: ab202468 IP in NIH/3T3 whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal -[EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab202468 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    All lanes: Immunoprecipitation - Anti-PDK1 antibody [EPR19571] (ab202468)

    Predicted band size: 49 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [EPR19571] (ab202468), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [EPR19571] (ab202468)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling PDK1 with ab202468 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on NIH/3T3 cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab202468 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.

    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-PDK1 antibody [EPR19571] (ab202468), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PDK1 antibody [EPR19571] (ab202468)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling PDK1 with ab202468 at 1/600 dilution (red) compared with a RabbitIgG,monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

    Cells were permeabilised by 90% methanol-1XPBS, -20°C, 30min.

  • Western blot - Anti-PDK1 antibody [EPR19571] (ab202468), expandable thumbnail

    Western blot - Anti-PDK1 antibody [EPR19571] (ab202468)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 minute; Lane 3-4: 10 seconds; Lane 5/6: 8 seconds.

    All lanes: Western blot - Anti-PDK1 antibody [EPR19571] (ab202468) at 1/2000 dilution

    Lane 1: L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lane 3: F9 (Mouse embryonic testicular cancer cell line) whole cell lysate

    Lane 4: bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate

    Lane 5: C6 (Rat glial tumor cell line) whole cell lysate

    Lane 6: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa

    Observed band size: 47 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [EPR19571] (ab202468), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [EPR19571] (ab202468)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling PDK1 with ab202468 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on RAW 264.7 cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab202468 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.

    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.

  • Western blot - Anti-PDK1 antibody [EPR19571] (ab202468), expandable thumbnail

    Western blot - Anti-PDK1 antibody [EPR19571] (ab202468)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/2/3/5/6: 8 seconds; Lane 4: 3 seconds; Lane 7: 1 second.

    All lanes: Western blot - Anti-PDK1 antibody [EPR19571] (ab202468) at 1/2000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Mouse kidney lysate at 10 µg

    Lane 3: Mouse spleen lysate at 10 µg

    Lane 4: Mouse heart lysate at 10 µg

    Lane 5: Rat brain lysate at 10 µg

    Lane 6: Rat kidney lysate at 10 µg

    Lane 7: Rat heart lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa

    Observed band size: 47 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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