Rabbit Recombinant Monoclonal PDK1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.
Images
![Western blot - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (AB251373), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr19571-bsa-and-azide-free-ab251373--western-blot-img140929.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (AB251373)")
![Immunoprecipitation - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (AB251373), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr19571-bsa-and-azide-free-ab251373--immunoprecipitation-img24793.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (AB251373)")
![Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (AB251373), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr19571-bsa-and-azide-free-ab251373--immunocytochemistry-immunofluorescence-img110963.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (AB251373)")
![Flow Cytometry (Intracellular) - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (AB251373), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr19571-bsa-and-azide-free-ab251373--flow-cytometry-intracellular-img27608.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (AB251373)")
![Western blot - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (AB251373), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr19571-bsa-and-azide-free-ab251373--western-blot-img56367.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (AB251373)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Expected | Expected | Tested | Expected |
| Mouse | Tested | Tested | Tested | Tested |
| Rat | Expected | Expected | Tested | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
Alternative names
PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1
Recommended products
Rabbit Recombinant Monoclonal PDK1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19571
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251373 is the carrier-free version of Anti-PDK1 antibody [EPR19571] ab202468.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr19571-bsa-and-azide-free-ab251373--western-blot-img140929.jpg "Western blot - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373)")
Western blot - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373)
This data was developed using Anti-PDK1 antibody [EPR19571] ab202468, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: PDK1 knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)
Lane 4: Jurkat whole cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-PDK1 antibody [EPR19571] ab202468 observed at 45 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-PDK1 antibody [EPR19571] ab202468 was shown to specifically react with PDK1 in wild-type cells along with additional cross reactive bands. Signal was lost in PDK1 knockout cells. Wild-type and PDK1 knockout samples were subjected to SDS-PAGE. Anti-PDK1 antibody [EPR19571] ab202468 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PDK1 antibody [EPR19571] (Anti-PDK1 antibody [EPR19571] ab202468)
Predicted band size: 49 kDa
![Immunoprecipitation - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr19571-bsa-and-azide-free-ab251373--immunoprecipitation-img24793.jpg "Immunoprecipitation - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373)")
Immunoprecipitation - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373)
This data was developed using Anti-PDK1 antibody [EPR19571] ab202468, the same antibody clone in a different buffer formulation.PDK1 was immunoprecipitated from 1mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with Anti-PDK1 antibody [EPR19571] ab202468 at 1/40 dilution. Western blot was performed from the immunoprecipitate using Anti-PDK1 antibody [EPR19571] ab202468 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution. Lane 1: NIH/3T3 whole cell lysate, 10µg (Input). Lane 2: Anti-PDK1 antibody [EPR19571] ab202468 IP in NIH/3T3 whole cell lysate. Lane 3: Rabbit IgG,monoclonal -[EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PDK1 antibody [EPR19571] ab202468 in NIH/3T3 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second.
All lanes: Immunoprecipitation - Anti-PDK1 antibody [EPR19571] (Anti-PDK1 antibody [EPR19571] ab202468)
Predicted band size: 49 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr19571-bsa-and-azide-free-ab251373--immunocytochemistry-immunofluorescence-img110963.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373)")
Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373)
This data was developed using Anti-PDK1 antibody [EPR19571] ab202468, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling PDK1 with Anti-PDK1 antibody [EPR19571] ab202468 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red). The negative controls are as follows:- -ve control 1: Anti-PDK1 antibody [EPR19571] ab202468 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution. -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
![Flow Cytometry (Intracellular) - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr19571-bsa-and-azide-free-ab251373--flow-cytometry-intracellular-img27608.jpg "Flow Cytometry (Intracellular) - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373)")
Flow Cytometry (Intracellular) - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373)
This data was developed using Anti-PDK1 antibody [EPR19571] ab202468, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling PDK1 with Anti-PDK1 antibody [EPR19571] ab202468 at 1/600 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.
Cells were permeabilised by 90% methanol-1XPBS, -20°C, 30min.
![Western blot - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr19571-bsa-and-azide-free-ab251373--western-blot-img56367.jpg "Western blot - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373)")
Western blot - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373)
This data was developed using Anti-PDK1 antibody [EPR19571] ab202468, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 1 minute; Lanes 3-4: 10 seconds; Lanes 5-6: 8 seconds.
All lanes: Western blot - Anti-PDK1 antibody [EPR19571] (Anti-PDK1 antibody [EPR19571] ab202468) at 1/2000 dilution
Lane 1: L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate
Lane 2: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 3: F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
Lane 4: bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate
Lane 5: C6 (Rat glial tumor cell line) whole cell lysate
Lane 6: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 49 kDa
Observed band size: 47 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr19571-bsa-and-azide-free-ab251373--immunocytochemistry-immunofluorescence-img108499.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373)")
Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373)
This data was developed using Anti-PDK1 antibody [EPR19571] ab202468, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling PDK1 with Anti-PDK1 antibody [EPR19571] ab202468 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on RAW 264.7 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red). The negative controls are as follows:- -ve control 1: Anti-PDK1 antibody [EPR19571] ab202468 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution. -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
![Western blot - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr19571-bsa-and-azide-free-ab251373--western-blot-img19731.jpg "Western blot - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373)")
Western blot - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373)
This data was developed using Anti-PDK1 antibody [EPR19571] ab202468, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-3, 5, and 6: 8 seconds; Lane 4: 3 seconds; Lane 7: 1 second.
All lanes: Western blot - Anti-PDK1 antibody [EPR19571] (Anti-PDK1 antibody [EPR19571] ab202468) at 1/2000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: Mouse kidney lysate at 10 µg
Lane 3: Mouse spleen lysate at 10 µg
Lane 4: Mouse heart lysate at 10 µg
Lane 5: Rat brain lysate at 10 µg
Lane 6: Rat kidney lysate at 10 µg
Lane 7: Rat heart lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 49 kDa
Observed band size: 47 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr19571-bsa-and-azide-free-ab251373--immunocytochemistry-immunofluorescence-img108499.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373)")
![Western blot - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr19571-bsa-and-azide-free-ab251373--western-blot-img19731.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PDK1 antibody [EPR19571] - BSA and Azide free (ab251373)")
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