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Rabbit Recombinant Monoclonal PDK1 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 19 publications.

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Images

Western blot - Anti-PDK1 antibody [EPR19573] (AB207450), expandable thumbnail
  • Immunoprecipitation - Anti-PDK1 antibody [EPR19573] (AB207450), expandable thumbnail
  • Western blot - Anti-PDK1 antibody [EPR19573] (AB207450), expandable thumbnail
  • Western blot - Anti-PDK1 antibody [EPR19573] (AB207450), expandable thumbnail
  • Western blot - Anti-PDK1 antibody [EPR19573] (AB207450), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/40
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

-

Species
Rat
Dilution info
1/2000
Notes

-

Species
Human
Dilution info
1/2000
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

Alternative names

PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1

Recommended products

Rabbit Recombinant Monoclonal PDK1 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 19 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19573
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-PDK1 antibody [EPR19573] (ab207450), expandable thumbnail

    Western blot - Anti-PDK1 antibody [EPR19573] (ab207450)

    Lanes 1- 2: Merged signal (red and green). Green - ab207450 observed at 45 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab207450 was shown to react with PDK1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264791 (knockout cell lysate ab257164) was used. Wild-type HeLa and PDK1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab207450 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-PDK1 antibody [EPR19573] (ab207450) at 1/2000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: PDK1 knockout HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 49 kDa

    Observed band size: 45 kDa

  • Immunoprecipitation - Anti-PDK1 antibody [EPR19573] (ab207450), expandable thumbnail

    Immunoprecipitation - Anti-PDK1 antibody [EPR19573] (ab207450)

    PDK1 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab207450 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab207450 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HepG2 whole cell lysate 10μg (Input).

    Lane 2: ab207450 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab207450 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    All lanes: Immunoprecipitation - Anti-PDK1 antibody [EPR19573] (ab207450)

    Predicted band size: 49 kDa

  • Western blot - Anti-PDK1 antibody [EPR19573] (ab207450), expandable thumbnail

    Western blot - Anti-PDK1 antibody [EPR19573] (ab207450)

    Lanes 1 - 4: Merged signal (red and green). Green - ab207450 observed at 45 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab207450 was shown to specifically react with PDK1 in wild-type HAP1 cells. No band was observed when PDK1 knockout samples were examined. Wild-type and PDK1 knockout samples were subjected to SDS-PAGE. ab207450 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted at 1/2000 and 1/10,000 dilution respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-PDK1 antibody [EPR19573] (ab207450) at 1/2000 dilution

    Lane 1: Wild-type HAP1 cell lysate at 20 µg

    Lane 2: PDK1 knockout HAP1 cell lysate at 20 µg

    Lane 3: HepG2 cell lysate at 20 µg

    Lane 4: LnCaP cell lysate at 20 µg

    Predicted band size: 49 kDa

  • Western blot - Anti-PDK1 antibody [EPR19573] (ab207450), expandable thumbnail

    Western blot - Anti-PDK1 antibody [EPR19573] (ab207450)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-5: 15 seconds; Lane 6: 8 seconds.

    All lanes: Western blot - Anti-PDK1 antibody [EPR19573] (ab207450) at 1/2000 dilution

    Lane 1: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

    Lane 2: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

    Lane 3: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

    Lane 4: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Lane 5: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

    Lane 6: LNCaP (Human prostate cancer cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa

    Observed band size: 47 kDa

  • Western blot - Anti-PDK1 antibody [EPR19573] (ab207450), expandable thumbnail

    Western blot - Anti-PDK1 antibody [EPR19573] (ab207450)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/3/4: 8 seconds; Lane 2: 30 seconds; Lane 5/6: 3 minutes; Lane 7/8: 15 seconds.

    All lanes: Western blot - Anti-PDK1 antibody [EPR19573] (ab207450) at 1/2000 dilution

    Lane 1: Human heart lysate at 10 µg

    Lane 2: Human kidney lysate at 10 µg

    Lane 3: Mouse brain lysate at 10 µg

    Lane 4: Mouse heart lysate at 10 µg

    Lane 5: Mouse kidney lysate at 10 µg

    Lane 6: Mouse spleen lysate at 10 µg

    Lane 7: Rat kidney lysate at 10 µg

    Lane 8: Rat spleen lysate at 10 µg

    Secondary

    Lanes 1 - 2: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Lanes 3 - 8: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa

    Observed band size: 47 kDa

  • Western blot - Anti-PDK1 antibody [EPR19573] (ab207450), expandable thumbnail

    Western blot - Anti-PDK1 antibody [EPR19573] (ab207450)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PDK1 antibody [EPR19573] (ab207450) at 1/5000 dilution

    Lane 1: HEK-293 (Human epithelial cell line from embryonic kidney) mitochondria fraction lysate at 10 µg

    Lane 2: HEK-293 (Human epithelial cell line from embryonic kidney) cytosol fraction lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa

    Observed band size: 47 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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