Rabbit Recombinant Monoclonal PDK1 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Rat, Human samples.
Images
![Western blot - Anti-PDK1 antibody [EPR19573] - BSA and Azide free (AB251478), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr19573-bsa-and-azide-free-ab251478--western-blot-img140445.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PDK1 antibody [EPR19573] - BSA and Azide free (AB251478)")
![Western blot - Anti-PDK1 antibody [EPR19573] - BSA and Azide free (AB251478), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr19573-bsa-and-azide-free-ab251478--western-blot-img58586.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PDK1 antibody [EPR19573] - BSA and Azide free (AB251478)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Expected | Predicted |
| Rat | Expected | Predicted |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
Alternative names
Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1, PDK1, PDHK1
Recommended products
Rabbit Recombinant Monoclonal PDK1 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Rat, Human samples.
Alternative names
Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1, PDK1, PDHK1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR19573
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab251478 is the carrier-free version of Anti-PDK1 antibody [EPR19573] ab207450.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PDK1 protein also known as 3-phosphoinositide-dependent protein kinase-1 is a kinase enzyme coded by the PDPK1 gene. PDK1 has a molecular weight of approximately 63 kDa. It is expressed across a variety of tissues including high levels in the heart pancreas liver skeletal muscle and in certain brain regions. This kinase plays a pivotal role in cellular signaling pathways due to its ability to phosphorylate other proteins.
- Biological function summary
PDK1 acts as a master regulator of the AGC family of kinases which includes proteins like PKB/Akt S6K and SGK. By phosphorylating these proteins PDK1 controls cell growth glycogen synthesis and apoptosis. It does not function within a complex but operates as an important activator in phosphorylation events influencing several downstream cellular responses. By modulating kinase activity PDK1 significantly impacts cellular metabolism and survival pathways.
- Pathways
PDK1 serves as a critical component in the PI3K/Akt signaling pathway and the mTOR pathway. In the PI3K/Akt pathway PDK1 phosphorylates and activates Akt leading to varied outcomes such as glucose uptake cell proliferation and resistance to apoptosis under stressful conditions. The relationship between PDK1 and other proteins like mTOR highlights its importance in regulating cell growth and survival through nutrient-sensing mechanisms.
- Associated diseases and disorders
PDK1 is heavily implicated in cancer development and diabetes. Aberrant activation of the PI3K/Akt/mTOR pathway often driven by mutations influencing PDK1 activity is a common feature in many cancers. PDK1's role in modulating insulin signaling also connects it to type 2 diabetes where insulin resistance is a characteristic issue. Through these pathways PDK1 interacts with oncogenes and proteins like PTEN further influencing disease progression and treatment outcomes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-PDK1 antibody [EPR19573] - BSA and Azide free (ab251478), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr19573-bsa-and-azide-free-ab251478--western-blot-img140445.jpg "Western blot - Anti-PDK1 antibody [EPR19573] - BSA and Azide free (ab251478)")
Western blot - Anti-PDK1 antibody [EPR19573] - BSA and Azide free (ab251478)
This data was developed using the same antibody clone in a different buffer formulation (Anti-PDK1 antibody [EPR19573] ab207450).
Lanes 1- 2: Merged signal (red and green). Green - Anti-PDK1 antibody [EPR19573] ab207450 observed at 45 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-PDK1 antibody [EPR19573] ab207450 was shown to react with PDK1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264791 (knockout cell lysate ab257164) was used. Wild-type HeLa and PDK1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-PDK1 antibody [EPR19573] ab207450 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PDK1 antibody [EPR19573] (Anti-PDK1 antibody [EPR19573] ab207450) at 1/2000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: PDK1 knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 45 kDa
![Western blot - Anti-PDK1 antibody [EPR19573] - BSA and Azide free (ab251478), expandable thumbnail](https://content.abcam.com/products/images/pdk1-antibody-epr19573-bsa-and-azide-free-ab251478--western-blot-img58586.jpg "Western blot - Anti-PDK1 antibody [EPR19573] - BSA and Azide free (ab251478)")
Western blot - Anti-PDK1 antibody [EPR19573] - BSA and Azide free (ab251478)
This data was developed using the same antibody clone in a different buffer formulation (Anti-PDK1 antibody [EPR19573] ab207450).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-PDK1 antibody [EPR19573] ab207450 observed at 45 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-PDK1 antibody [EPR19573] ab207450 was shown to specifically react with PDK1 in wild-type HAP1 cells. No band was observed when PDK1 knockout samples were examined. Wild-type and PDK1 knockout samples were subjected to SDS-PAGE. Anti-PDK1 antibody [EPR19573] ab207450 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted at 1/2000 and 1/10,000 dilution respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PDK1 antibody [EPR19573] - BSA and Azide free (ab251478) at 1/2000 dilution
Lane 1: Wild-type HAP1 cell lysate at 20 µg
Lane 2: PDK1 knockout HAP1 cell lysate at 20 µg
Lane 3: HepG2 cell lysate at 20 µg
Lane 4: LnCaP cell lysate at 20 µg
Predicted band size: 49 kDa
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