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AB326584

Anti-PDK1 antibody [SP270] - BSA and Azide free

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Rabbit Recombinant Monoclonal PDK1 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.

View Alternative Names

PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - BSA and Azide free (AB326584)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - BSA and Azide free (AB326584)

This data was developed using ab227682, the same antibody clone in a different buffer formulation.

Formalin-fixed, paraffin-embedded human stomach tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - BSA and Azide free (AB326584)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - BSA and Azide free (AB326584)

This data was developed using ab227682, the same antibody clone in a different buffer formulation.

Formalin-fixed, paraffin-embedded human liver tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - BSA and Azide free (AB326584)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - BSA and Azide free (AB326584)

This data was developed using ab227682, the same antibody clone in a different buffer formulation.

Formalin-fixed, paraffin-embedded human kidney tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - BSA and Azide free (AB326584)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - BSA and Azide free (AB326584)

This data was developed using ab227682, the same antibody clone in a different buffer formulation.

Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - BSA and Azide free (AB326584)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - BSA and Azide free (AB326584)

This data was developed using ab227682, the same antibody clone in a different buffer formulation.

Formalin-fixed, paraffin-embedded human heart tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - BSA and Azide free (AB326584)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - BSA and Azide free (AB326584)

This data was developed using ab227682, the same antibody clone in a different buffer formulation.

Formalin-fixed, paraffin-embedded human pancreas tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - BSA and Azide free (AB326584)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - BSA and Azide free (AB326584)

This data was developed using ab227682, the same antibody clone in a different buffer formulation.

Formalin-fixed, paraffin-embedded human testis tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP270

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.</p><p>Primary antibody incubation for 10 minutes at room temperature.</p>" } } }
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Product details

ab326584 is the carrier-free version of ab227682

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2 (PubMed : 7499431, PubMed : 18541534, PubMed : 22195962, PubMed : 26942675, PubMed : 17683942). This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate (PubMed : 18541534, PubMed : 22195962, PubMed : 26942675). Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia (PubMed : 18541534, PubMed : 22195962, PubMed : 26942675).
See full target information PDK1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com