AB227682

Anti-PDK1 antibody [SP270] - C-terminal

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal PDK1 antibody. C-terminal. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - C-terminal (AB227682)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - C-terminal (AB227682)

Formalin-fixed, paraffin-embedded human liver tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - C-terminal (AB227682)

Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - C-terminal (AB227682)

Formalin-fixed, paraffin-embedded human kidney tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - C-terminal (AB227682)

Formalin-fixed, paraffin-embedded human testis tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - C-terminal (AB227682)

Formalin-fixed, paraffin-embedded human stomach tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - C-terminal (AB227682)

Formalin-fixed, paraffin-embedded human heart tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK1 antibody [SP270] - C-terminal (AB227682)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP270

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.</p><p>Primary antibody incubation for 10 minutes at room temperature.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by Protein A/G.

Storage buffer

pH: 7.6 Preservative: 0.099% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

See full target information PDK1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 38:1129-1143 PubMed36331285

2022

Circ_0000284 facilitates the growth, metastasis and glycolysis of intrahepatic cholangiocarcinoma through miR-152-3p-mediated PDK1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Sun,Menglong Feng,Huilian Zou,Yanping Mao,Wei Yu

Oncology letters 18:545-552 PubMed31289526

2019

Lymphoepithelioma-like gastric carcinoma treated with partial gastrectomy: Two case reports.

Applications

Unspecified application

Species

Unspecified reactive species

Huihua Cao,Jun Xie,Yongxiang Qian,Yugang Wu,Zhaoqing Tang

View all publications

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