AB208187

Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal PDK4 antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human, Recombinant fragment - Mouse samples. Cited in 2 publications.

View Alternative Names

PDHK4, PDK4, Pyruvate dehydrogenase kinase isoform 4, PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1, PDHK2, PDK2, Pyruvate dehydrogenase kinase isoform 2, PDH kinase 2, PDKII

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Supplier Data

Immunoprecipitation - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (AB208187)

PDK1+PDK2+PDK4 was immunoprecipitated from 0.35 mg of Mouse skeletal muscle whole cell lysate with ab208187 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab208187 at 1/500 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Mouse skeletal muscle whole cell lysate 10μg (Input).

Lane 2 : ab208187 IP in Mouse skeletal muscle whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab208187 in Mouse skeletal muscle whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187)

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Supplier Data

Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (AB208187)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1-4 : 10 seconds; Lane 5-6 : 3 minutes; Lane 7 : 2 seconds.

All lanes:

Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Mouse liver tissue lysate at 10 µg

Lane 4:

C2C12 (Mouse myoblast cell line) whole cell lysate at 10 µg

Lane 5:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 6:

Human heart lysate at 10 µg

Lane 7:

Rat heart tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

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Supplier Data

Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (AB208187)

Blocking/Dilution buffer : 5% NFDM/TBST.

Mouse PDK4, PDK1 and PDK2 recombinant proteins contain aa8-412, aa1-434 and aa1-407 respectively with a His-Tag®.

All lanes:

Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187) at 1/10000 dilution

Lane 1:

Mouse PDK4 recombinant protein at 0.01 µg

Lane 2:

Mouse PDK1 recombinant protein at 0.01 µg

Lane 3:

Mouse PDK2 recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

false

Exposure time: 1s

Carrier free

Anti-PDK4 antibody [EPR19727] - BSA and Azide free (Capture)
Carrier free

Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19727

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Recombinant fragment - Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein targets PDK1 PDK2 and PDK4 short for pyruvate dehydrogenase kinase 1 2 and 4 are enzymes that regulate the activity of the pyruvate dehydrogenase complex (PDC) by phosphorylation inhibiting its enzymatic function. PDK1 has a mass of approximately 49 kDa PDK2 about 46 kDa and PDK4 around 48 kDa. These kinases are expressed in various tissues with PDK2 showing expression in heart pancreas and kidney while PDK4 expression is more observed in skeletal muscle and liver. PDK1 is ubiquitously expressed.

Biological function summary

These kinases play key roles in controlling cellular metabolism by inhibiting the conversion of pyruvate to acetyl-CoA a critical step linking glycolysis to the tricarboxylic acid (TCA) cycle. Acting separately but in functionally similar ways to each other PDK1 PDK2 and PDK4 help regulate energy homeostasis and preserve glucose during starvation or limited oxygen supply. These kinases are also part of larger complexes and interact with the pyruvate dehydrogenase complex and molecules like NADH and acetyl-CoA that further modulate their activity.

Pathways

PDK1 PDK2 and PDK4 are integral to the metabolic pathways that control glucose metabolism and TCA cycle by modulating pyruvate dehydrogenase complex activity. These kinases interact with signaling pathways in response to hormonal and nutrient signals adapting the body's energy metabolism under varying conditions. The insulin signaling pathway and hypoxia-inducible factor (HIF) pathway significantly influence their activity. Insulin signaling decreases the activity of PDK leading to an increase in pyruvate dehydrogenase activity and therefore in glucose oxidation. Meanwhile HIF pathway activation in low oxygen conditions upregulates PDKs to promote anaerobic metabolism.

The dysregulation of PDK1 PDK2 and PDK4 is linked to metabolic conditions such as diabetes and cancer. In diabetes an upregulation of PDK activity can lead to reduced glucose oxidation and increased blood glucose levels often connected to insulin resistance. PDKs suppress he activity of the pyruvate dehydrogenase complex leading to altered energy metabolism that is a hallmark in many cancers. Proteins such as insulin receptor substrate (IRS) in diabetes and hypoxia-inducible factor-1α (HIF-1α) in cancer are partners that link PDK activity to these disease states as they modulate PDK expression and activity in response to metabolic demands.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.

See full target information PDK4

Additional targets

PDK1,PDK2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Arteriosclerosis, thrombosis, and vascular biology 43:1851-1866 PubMed37589135

2023

Plasminogen Receptors Promote Lipoprotein(a) Uptake by Enhancing Surface Binding and Facilitating Macropinocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Halima Siddiqui,Nikita Deo,Malcolm T Rutledge,Michael J A Williams,Gregory M I Redpath,Sally P A McCormick

Oncology research 26:869-878 PubMed29212574

2017

Overexpression of Long Noncoding RNA PTENP1 Inhibits Cell Proliferation and Migration via Suppression of miR-19b in Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xianbiao Shi,Xiaoqiao Tang,Lei Su

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727]

Immunoprecipitation - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (AB208187)

Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (AB208187)

Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (AB208187)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (2)

Product promise