AB201964

Anti-PDK2 antibody [3F2D7]

Name: Anti-PDK2 antibody [3F2D7] (ab201964) | Abcam
Brand: Abcam Limited
SKU: AB201964
Rating: 4 (1 reviews)

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(1 Publication)

Mouse Monoclonal PDK2 antibody. Suitable for Flow Cyt, WB and reacts with Human, Rat, African green monkey, Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PDK2 aa 150 to C-terminus.

View Alternative Names

PDHK2, PDK2, Pyruvate dehydrogenase kinase isoform 2, PDH kinase 2, PDKII

5 Images

Flow Cytometry - Anti-PDK2 antibody [3F2D7] (AB201964)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-PDK2 antibody [3F2D7] (AB201964)

Flow cytometric analysis of A431 cells labeling PDK2 with ab201964 at 1/200 dilution (green). Negative control (red).

Lab

Western blot - Anti-PDK2 antibody [3F2D7] (AB201964)

Western blot : Mouse Monoclonal [3F2D7] to PDK2 ab201964 staining at 1/500 dilution, shown in green; Rabbit anti GAPDH ab181602 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 45 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in PDK2 knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse 800CW and Goat anti-Rabbit 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-PDK2 antibody [3F2D7] (ab201964) at 1/500 dilution

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

PDK2 knockout U-87 MG at 20 µg

Lane 3:

SH-SY5Y at 20 µg

Lane 4:

HL-60 at 20 µg

Secondary

All lanes:

Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20000 dilution

Predicted band size: 46 kDa

Observed band size: 45 kDa,36 kDa

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Supplier Data

Western blot - Anti-PDK2 antibody [3F2D7] (AB201964)

All lanes:

Western blot - Anti-PDK2 antibody [3F2D7] (ab201964) at 1/500 dilution

All lanes:

Human recombinant PDK2 (aa178-404) protein

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-PDK2 antibody [3F2D7] (AB201964)

All lanes:

Western blot - Anti-PDK2 antibody [3F2D7] (ab201964) at 1/500 dilution

Lane 1:

Jurkat cell lysate

Lane 2:

C6 cell lysate

Lane 3:

COS7 cell lysate

Lane 4:

K562 cell lysate

Lane 5:

A431 cell lysate

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-PDK2 antibody [3F2D7] (AB201964)

All lanes:

Western blot - Anti-PDK2 antibody [3F2D7] (ab201964) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

Human recombinant PDK2 (aa178-404)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 46 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3F2D7

Isotype

IgG2b

Carrier free

Reacts with

Rat, Human, African green monkey

Applications

Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human PDK2 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15119

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "African green monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDK2 also known as Pyruvate Dehydrogenase Kinase 2 is a mitochondrial protein involved in the regulation of glucose metabolism. It phosphorylates and inactivates the pyruvate dehydrogenase complex (PDC) which plays an important role in controlling the conversion of pyruvate to acetyl-CoA. PDK2 has a molecular mass of approximately 46 kDa and shows expression in various tissues including the heart liver and kidneys. The expression pattern indicates its significance in tissues with high metabolic demands.

Biological function summary

PDK2 regulates cellular energy metabolism by phosphorylating the E1 component of PDC resulting in lowered acetyl-CoA production. PDK2 often acts as part of a regulatory complex with PDC influencing metabolic flux within the mitochondria. Its activity can alter ATP production and fatty acid synthesis impacting overall cellular function and energy homeostasis. In response to different metabolic needs the availability and activity of PDK2 modulate the balance between carbohydrate utilization and lipid oxidation.

Pathways

PDK2 has an important role in the glucose and lipid metabolic pathways by interacting with PDC. The pathways where PDK2 is active are essential for maintaining energy homeostasis and are sensitive to different physiological conditions. PDK2's regulation of pyruvate oxidation influences the tricarboxylic acid (TCA) cycle affecting key metabolic enzymes such as pyruvate dehydrogenase phosphatase 1 (PDP1) and PDK1. Its activity is often fine-tuned by factors impacting energy needs insulin signaling and nutrient availability.

Abnormal PDK2 activity associates with metabolic conditions like diabetes and cancer. In diabetes the dysregulation of PDK2 alters the metabolic processing of carbohydrates leading to increased reliance on lipid oxidation and contributing to hyperglycemia. In cancer PDK2's role in promoting aerobic glycolysis also known as the Warburg effect supports rapid cell growth and survival. The link between PDK2 and these diseases highlights its involvement alongside other proteins such as hypoxia-inducible factor 1-alpha (HIF-1α) in promoting altered energy metabolism in pathological states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis.

See full target information PDK2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 9:9998 PubMed31292492

2019

Characterisation of a human antibody that potentially links cytomegalovirus infection with systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Ying Jacklyn Neo,Seng Yin Kelly Wee,Isabelle Bonne,Sen Hee Tay,Manfred Raida,Vojislav Jovanovic,Anna-Marie Fairhurst,Jinhua Lu,Brendon J Hanson,Paul A MacAry

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDK2 antibody [3F2D7]

Flow Cytometry - Anti-PDK2 antibody [3F2D7] (AB201964)

Western blot - Anti-PDK2 antibody [3F2D7] (AB201964)

Western blot - Anti-PDK2 antibody [3F2D7] (AB201964)

Western blot - Anti-PDK2 antibody [3F2D7] (AB201964)

Western blot - Anti-PDK2 antibody [3F2D7] (AB201964)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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