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AB246888

Anti-PDK2 antibody

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(1 Publication)

Rabbit Polyclonal PDK2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PDK2 aa 1-200.

View Alternative Names

PDHK2, PDK2, Pyruvate dehydrogenase kinase isoform 2, PDH kinase 2, PDKII

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK2 antibody (AB246888)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK2 antibody (AB246888)

Paraffin-embedded human heart muscle tissue stained for PDK2 using ab246888 at 1/20 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-PDK2 antibody (AB246888)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PDK2 antibody (AB246888)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for PDK2 (Green) using ab246888 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PDK2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q15119

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Complementary Products

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Anti-PDK1 antibody [EPR19571]

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Primary Antibodies

AB110330

Anti-PDHA1 antibody [9H9AF5]

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Primary Antibodies

AB214938

Anti-PDK4 antibody [EPR19727-245]

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Proteins & Peptides

AB125592

Recombinant human PDK2 protein

Functional Studies - Recombinant human PDK2 protein (AB125592)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDK2 also known as Pyruvate Dehydrogenase Kinase 2 is a mitochondrial protein involved in the regulation of glucose metabolism. It phosphorylates and inactivates the pyruvate dehydrogenase complex (PDC) which plays an important role in controlling the conversion of pyruvate to acetyl-CoA. PDK2 has a molecular mass of approximately 46 kDa and shows expression in various tissues including the heart liver and kidneys. The expression pattern indicates its significance in tissues with high metabolic demands.
Biological function summary

PDK2 regulates cellular energy metabolism by phosphorylating the E1 component of PDC resulting in lowered acetyl-CoA production. PDK2 often acts as part of a regulatory complex with PDC influencing metabolic flux within the mitochondria. Its activity can alter ATP production and fatty acid synthesis impacting overall cellular function and energy homeostasis. In response to different metabolic needs the availability and activity of PDK2 modulate the balance between carbohydrate utilization and lipid oxidation.

Pathways

PDK2 has an important role in the glucose and lipid metabolic pathways by interacting with PDC. The pathways where PDK2 is active are essential for maintaining energy homeostasis and are sensitive to different physiological conditions. PDK2's regulation of pyruvate oxidation influences the tricarboxylic acid (TCA) cycle affecting key metabolic enzymes such as pyruvate dehydrogenase phosphatase 1 (PDP1) and PDK1. Its activity is often fine-tuned by factors impacting energy needs insulin signaling and nutrient availability.

Abnormal PDK2 activity associates with metabolic conditions like diabetes and cancer. In diabetes the dysregulation of PDK2 alters the metabolic processing of carbohydrates leading to increased reliance on lipid oxidation and contributing to hyperglycemia. In cancer PDK2's role in promoting aerobic glycolysis also known as the Warburg effect supports rapid cell growth and survival. The link between PDK2 and these diseases highlights its involvement alongside other proteins such as hypoxia-inducible factor 1-alpha (HIF-1α) in promoting altered energy metabolism in pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis.
See full target information PDK2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101758 PubMed39368479

2024

CDK12 loss drives prostate cancer progression, transcription-replication conflicts, and synthetic lethality with paralog CDK13.

Applications

Unspecified application

Species

Unspecified reactive species

Jean Ching-Yi Tien,Jie Luo,Yu Chang,Yuping Zhang,Yunhui Cheng,Xiaoju Wang,Jianzhang Yang,Rahul Mannan,Somnath Mahapatra,Palak Shah,Xiao-Ming Wang,Abigail J Todd,Sanjana Eyunni,Caleb Cheng,Ryan J Rebernick,Lanbo Xiao,Yi Bao,James Neiswender,Rachel Brough,Stephen J Pettitt,Xuhong Cao,Stephanie J Miner,Licheng Zhou,Yi-Mi Wu,Estefania Labanca,Yuzhuo Wang,Abhijit Parolia,Marcin Cieslik,Dan R Robinson,Zhen Wang,Felix Y Feng,Jonathan Chou,Christopher J Lord,Ke Ding,Arul M Chinnaiyan
View all publications
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Product promise

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