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AB154549

Anti-PDK3 antibody

0

(1 Review)

|

(7 Publications)

Rabbit Polyclonal PDK3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Recombinant fragment samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PDK3 aa 1-300.

View Alternative Names

PDHK3, PDK3, Pyruvate dehydrogenase kinase isoform 3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK3 antibody (AB154549)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK3 antibody (AB154549)

Immunohistochemical analysis of paraffin-embedded Human Ca922 xenograft tissue labeling PDK3 with ab154549 at 1/100 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-PDK3 antibody (AB154549)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PDK3 antibody (AB154549)

Immunofluorescent analysis of methanol-fixed HepG2 cells labeling PDK3 with ab154549 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

Western blot - Anti-PDK3 antibody (AB154549)
  • WB

Supplier Data

Western blot - Anti-PDK3 antibody (AB154549)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PDK3 antibody (ab154549) at 1/1000 dilution

All lanes:

HepG2 whole cell lysates at 30 µg

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PDK3 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q15120

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Complementary Products

Assay Kits

AB109902

Pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit

Functional Studies - Pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit (AB109902)

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Primary Antibodies

AB68164

Anti-PDK2 antibody [EPR1948Y]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDK2 antibody [EPR1948Y] (AB68164)

  • 5
  • 2
Primary Antibodies

AB92696

Anti-PDHA1 (phospho S293) antibody

BOND RX™ Validated

Western blot - Anti-PDHA1 (phospho S293) antibody (AB92696)

  • 5
  • 11
Primary Antibodies

AB202468

Anti-PDK1 antibody [EPR19571]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [EPR19571] (AB202468)

  • 0
  • 0
Primary Antibodies

AB110336

Anti-PDK4 antibody [1C2BG5]

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PDK4 antibody [1C2BG5] (AB110336)

  • 3
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDK3 also known as Pyruvate Dehydrogenase Kinase Isozyme 3 plays a mechanical role in regulating the activity of the pyruvate dehydrogenase (PDH) complex by phosphorylation. It phosphorylates the E1 component of the PDH complex which inhibits PDH activity and reduces the conversion of pyruvate to acetyl-CoA. PDK3 has a molecular mass of approximately 47 kDa and is mainly expressed in skeletal muscle and heart tissues.
Biological function summary

This kinase functions as an important modulator of glucose metabolism. PDK3 acts within the mitochondrial matrix interacting with its substrate as part of the larger PDH complex. Through its regulatory influence PDK3 helps maintain cellular energy homeostasis by balancing usage of carbohydrates and lipids depending on metabolic demands and availability of nutrients.

Pathways

PDK3 integrates into critical energy metabolism networks such as the tricarboxylic acid (TCA) cycle and the gluconeogenesis pathway. It regulates the PDH complex by inhibition impacting the flow of carbon through these pathways. PDK3's activity relates closely with other PDK isoforms such as PDK1 PDK2 and PDK4 which also modulate PDH complex function depending on tissue-specific and metabolic conditions.

PDK3 holds significance in diabetes and cancer. In type 2 diabetes PDK3's inhibition of PDH can contribute to impaired glucose oxidation leading to elevated blood glucose levels. In cancer the upregulation of PDK3 may support the metabolic switch to aerobic glycolysis a hallmark of many tumors commonly known as the Warburg effect. The activity of PDK3 in these conditions connects with lactate dehydrogenase (LDH) in cancer as both enzymes participate in metabolic reprogramming favoring lactate production.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species.
See full target information PDK3
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell metabolism 35:1163-1178.e10 PubMed37327791

2023

Acetate controls endothelial-to-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong Zhu,Yunyun Wang,Ioana Soaita,Heon-Woo Lee,Hosung Bae,Nabil Boutagy,Anna Bostwick,Rong-Mo Zhang,Caitlyn Bowman,Yanying Xu,Sophie Trefely,Yu Chen,Lingfeng Qin,William Sessa,George Tellides,Cholsoon Jang,Nathaniel W Snyder,Luyang Yu,Zoltan Arany,Michael Simons

Molecular medicine reports 27: PubMed36799154

2023

Effect of miR‑29a‑3p in exosomes on glioma cells by regulating the PI3K/AKT/HIF‑1α pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zeqiang Liu,Zheng Yang,Lu He

Experimental & molecular medicine 54:1940-1954 PubMed36369467

2022

Hypoxia-induced circRNF13 promotes the progression and glycolysis of pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuyan Zhao,Zhonglin Zhu,Wenqin Xiao,Guanzhao Zong,Chuanyang Wang,Weiliang Jiang,Kai Li,Jie Shen,Xingya Guo,Jianhua Cui,Lihong Guo,Rong Wan

Nature metabolism 4:693-710 PubMed35760868

2022

Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Emily J Kay,Karla Paterson,Carla Riera-Domingo,David Sumpton,J Henry M Däbritz,Saverio Tardito,Claudia Boldrini,Juan R Hernandez-Fernaud,Dimitris Athineos,Sandeep Dhayade,Ekaterina Stepanova,Enio Gjerga,Lisa J Neilson,Sergio Lilla,Ann Hedley,Grigorios Koulouras,Grace McGregor,Craig Jamieson,Radia Marie Johnson,Morag Park,Kristina Kirschner,Crispin Miller,Jurre J Kamphorst,Fabricio Loayza-Puch,Julio Saez-Rodriguez,Massimiliano Mazzone,Karen Blyth,Michele Zagnoni,Sara Zanivan

Cancers 13: PubMed33375130

2020

Metabolic Reprogramming by Malat1 Depletion in Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Simona Nanni,Aurora Aiello,Chiara Salis,Agnese Re,Chiara Cencioni,Lorenza Bacci,Francesco Pierconti,Francesco Pinto,Cristian Ripoli,Paola Ostano,Silvia Baroni,Giacomo Lazzarino,Barbara Tavazzi,Dario Pugliese,PierFrancesco Bassi,Claudio Grassi,Simona Panunzi,Giovanna Chiorino,Alfredo Pontecorvi,Carlo Gaetano,Antonella Farsetti

Cell reports 28:2317-2330.e8 PubMed31461649

2019

Synthetic Essentiality of Metabolic Regulator PDHK1 in PTEN-Deficient Cells and Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Nilanjana Chatterjee,Evangelos Pazarentzos,Manasi K Mayekar,Philippe Gui,David V Allegakoen,Gorjan Hrustanovic,Victor Olivas,Luping Lin,Erik Verschueren,Jeffrey R Johnson,Matan Hofree,Jenny J Yan,Billy W Newton,John V Dollen,Charles H Earnshaw,Jennifer Flanagan,Elton Chan,Saurabh Asthana,Trey Ideker,Wei Wu,Junji Suzuki,Benjamin A Barad,Yuriy Kirichok,James S Fraser,William A Weiss,Nevan J Krogan,Asmin Tulpule,Amit J Sabnis,Trever G Bivona

Cell reports 16:3016-3027 PubMed27626669

2016

KDM4A Coactivates E2F1 to Regulate the PDK-Dependent Metabolic Switch between Mitochondrial Oxidation and Glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Yu Wang,Chiu-Lien Hung,Yun-Ru Chen,Joy C Yang,Junjian Wang,Mel Campbell,Yoshihiro Izumiya,Hong-Wu Chen,Wen-Ching Wang,David K Ann,Hsing-Jien Kung
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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