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Rabbit Recombinant Monoclonal PDK4 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Recombinant fragment - Mouse samples. Cited in 26 publications.


Images

Western blot - Anti-PDK4 antibody [EPR19727-245] (AB214938), expandable thumbnail
  • Western blot - Anti-PDK4 antibody [EPR19727-245] (AB214938), expandable thumbnail
  • Western blot - Anti-PDK4 antibody [EPR19727-245] (AB214938), expandable thumbnail
  • Immunoprecipitation - Anti-PDK4 antibody [EPR19727-245] (AB214938), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PDK4 antibody [EPR19727-245] (AB214938), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBFlow Cyt (Intra)
Mouse
Tested
Tested
Tested
Rat
Expected
Tested
Expected
Recombinant fragment - Mouse
Not recommended
Tested
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Mouse
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/60
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.

Alternative names

Pyruvate dehydrogenase kinase isoform 4, Pdk4

Recommended products

Rabbit Recombinant Monoclonal PDK4 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Recombinant fragment - Mouse samples. Cited in 26 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19727-245
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PDK4 also known as Pyruvate Dehydrogenase Kinase Isozyme 4 plays an essential role in regulating mitochondrial energy metabolism. PDK4 phosphorylates the pyruvate dehydrogenase complex controlling the transformation of pyruvate to acetyl-CoA a critical step in energy production. The protein has a molecular weight of around 49 kDa. PDK4 expression occurs in various tissues with high levels observed in skeletal muscle heart and liver reflecting its role in energy metabolic processes.

Biological function summary

The enzyme functions to inhibit the pyruvate dehydrogenase complex by adding phosphate groups which reduces the conversion of pyruvate to acetyl-CoA and shifts energy production from carbohydrates to fats especially during fasting or prolonged exercise. PDK4 operates as a homodimer and is often part of a larger regulatory system balancing glucose and fatty acid oxidation in response to nutrient availability.

Pathways

PDK4 is integral to the regulation of both glucose metabolism and fatty acid oxidation. The enzyme interacts closely with the insulin signaling pathway where it modulates glucose uptake and utilization based on hormonal signals. Additionally PDK4 is a part of the hypoxia-inducible factor-1 (HIF-1) pathway where it plays a role in cellular response to low oxygen levels interacting with related proteins such as pyruvate dehydrogenase (PDH).

Associated diseases and disorders

PDK4's activity is linked to metabolic conditions like diabetes and obesity where its altered expression can lead to insulin resistance by affecting glucose utilization. Furthermore PDK4 is associated with cardiovascular diseases through its influence on cardiac energy metabolism and its interaction with other proteins such as PDH contributing to heart dysfunction when deregulated.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938), expandable thumbnail

    Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Mouse PDK4, PDK1 and PDK2 recombinant proteins contain aa8-412, aa1-434 and aa1-407 respectively with a His-Tag®.

    All lanes: Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938) at 1/10000 dilution

    Lane 1: Mouse PDK4 recombinant protein at 0.01 µg

    Lane 2: Mouse PDK1 recombinant protein at 0.01 µg

    Lane 3: Mouse PDK2 recombinant protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 46 kDa

    Observed band size: 47 kDa

    Exposure time: 5s

  • Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938), expandable thumbnail

    Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 5 seconds; Lane 2-4: 3 minutes; Lane 5: 1 minutes; Lane 6: 10 seconds.

    All lanes: Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938) at 1/1000 dilution

    Lane 1: Mouse skeletal muscle lysate at 10 µg

    Lane 2: Mouse heart lysate at 10 µg

    Lane 3: Rat muscle lysate at 10 µg

    Lane 4: Rat heart lysate at 10 µg

    Lane 5: C2C12 (Mouse myoblast cell line) whole cell lysate at 10 µg

    Lane 6: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 46 kDa

    Observed band size: 47 kDa

  • Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938), expandable thumbnail

    Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression level of PDK4 is increased under the fasting state (PMID: 11723055 and PMID: 16606348).

    Lanes 1 - 2: Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938) at 1/10000 dilution

    Lanes 3 - 4: Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938) at 1/1000 dilution

    Lane 1: Heart from nonfasting mouse at 10 µg

    Lane 2: Heart from mouse fasting for 72 hours at 10 µg

    Lane 3: Gastrocnemius muscle from nonfasting mouse at 10 µg

    Lane 4: Gastrocnemius muscle from mouse fasting for 72 hours at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 46 kDa

    Observed band size: 47 kDa

    Exposure time: 5s

  • Immunoprecipitation - Anti-PDK4 antibody [EPR19727-245] (ab214938), expandable thumbnail

    Immunoprecipitation - Anti-PDK4 antibody [EPR19727-245] (ab214938)

    PDK4 was immunoprecipitated from 0.35 mg of mouse heart lysate with ab214938 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab214938 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution

    Lane 1: Mouse heart lysate 10μg (Input).

    Lane 2: ab214938 IP in mouse heart lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab214938 in mouse heart lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 0.5 second.

    All lanes: Immunoprecipitation - Anti-PDK4 antibody [EPR19727-245] (ab214938)

    Predicted band size: 46 kDa, 48 kDa

  • Flow Cytometry (Intracellular) - Anti-PDK4 antibody [EPR19727-245] (ab214938), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PDK4 antibody [EPR19727-245] (ab214938)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed C2C12 (Mouse myoblast cell line) cells labeling PDK4 with ab214938 at 1/60 dilution (red) compared withRabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-PDK4 antibody [EPR19727-245] (ab214938)

    PDK4 western blot using anti-PDK4 antibody [EPR19727-245] ab214938. Publication image and figure legend from Gao, K., Zhang, J., et al., 2020, Chin Med, PubMed 32158496.


    ab214938 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab214938 please see the product overview.

    Effects of QSG on the uncoupling of glycolysis from glucose oxidation in HF rats after AMI. a Lactate level in serum and myocardial tissue. Lactate level in serum and myocardial tissue was significantly increased following HF and downregulated with QSG treatment. n = 8 per group. Values are mean ± SE. Asterisks indicates significant differences. *P < 0.05, **P < 0.01. b Myocardial protein expressional levels of LDHA and PDK4 in the three groups. LDHA involved in regulating glycolysis was observably elevated in the model group. LDHA level was significantly down-regulated by treatment of QSG compared with the model group. PDK4, decreasing glucose oxidation by an inhibitory phosphorylation of the pyruvate dehydrogenase complex, was up-regulated in the model group and down-regulated in the QSG group. n = 4 per group. Values are mean ± SE. Asterisks indicates significant differences. *P < 0.05, **P < 0.01

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