Rabbit Recombinant Monoclonal PDK4 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Recombinant fragment - Mouse samples.
Images
![Immunoprecipitation - Anti-PDK4 antibody [EPR19727-245] - BSA and Azide free (AB222401), expandable thumbnail](https://content.abcam.com/products/images/pdk4-antibody-epr19727-245-bsa-and-azide-free-ab222401--immunoprecipitation-img15826.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PDK4 antibody [EPR19727-245] - BSA and Azide free (AB222401)")
![Flow Cytometry (Intracellular) - Anti-PDK4 antibody [EPR19727-245] - BSA and Azide free (AB222401), expandable thumbnail](https://content.abcam.com/products/images/pdk4-antibody-epr19727-245-bsa-and-azide-free-ab222401--flow-cytometry-intracellular-img138687.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PDK4 antibody [EPR19727-245] - BSA and Azide free (AB222401)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Mouse | Tested | Tested | Tested |
| Rat | Expected | Tested | Expected |
| Recombinant fragment - Mouse | Not recommended | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.
Alternative names
Pyruvate dehydrogenase kinase isoform 4, Pdk4
Recommended products
Rabbit Recombinant Monoclonal PDK4 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Recombinant fragment - Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19727-245
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab222401 is the carrier-free version of Anti-PDK4 antibody [EPR19727-245] ab214938.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDK4 also known as Pyruvate Dehydrogenase Kinase Isozyme 4 plays an essential role in regulating mitochondrial energy metabolism. PDK4 phosphorylates the pyruvate dehydrogenase complex controlling the transformation of pyruvate to acetyl-CoA a critical step in energy production. The protein has a molecular weight of around 49 kDa. PDK4 expression occurs in various tissues with high levels observed in skeletal muscle heart and liver reflecting its role in energy metabolic processes.
- Biological function summary
The enzyme functions to inhibit the pyruvate dehydrogenase complex by adding phosphate groups which reduces the conversion of pyruvate to acetyl-CoA and shifts energy production from carbohydrates to fats especially during fasting or prolonged exercise. PDK4 operates as a homodimer and is often part of a larger regulatory system balancing glucose and fatty acid oxidation in response to nutrient availability.
- Pathways
PDK4 is integral to the regulation of both glucose metabolism and fatty acid oxidation. The enzyme interacts closely with the insulin signaling pathway where it modulates glucose uptake and utilization based on hormonal signals. Additionally PDK4 is a part of the hypoxia-inducible factor-1 (HIF-1) pathway where it plays a role in cellular response to low oxygen levels interacting with related proteins such as pyruvate dehydrogenase (PDH).
- Associated diseases and disorders
PDK4's activity is linked to metabolic conditions like diabetes and obesity where its altered expression can lead to insulin resistance by affecting glucose utilization. Furthermore PDK4 is associated with cardiovascular diseases through its influence on cardiac energy metabolism and its interaction with other proteins such as PDH contributing to heart dysfunction when deregulated.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunoprecipitation - Anti-PDK4 antibody [EPR19727-245] - BSA and Azide free (ab222401), expandable thumbnail](https://content.abcam.com/products/images/pdk4-antibody-epr19727-245-bsa-and-azide-free-ab222401--immunoprecipitation-img15826.jpg "Immunoprecipitation - Anti-PDK4 antibody [EPR19727-245] - BSA and Azide free (ab222401)")
Immunoprecipitation - Anti-PDK4 antibody [EPR19727-245] - BSA and Azide free (ab222401)
PDK4 was immunoprecipitated from 0.35 mg of mouse heart lysate with Anti-PDK4 antibody [EPR19727-245] ab214938 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using Anti-PDK4 antibody [EPR19727-245] ab214938 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse heart lysate 10μg (Input).
Lane 2: Anti-PDK4 antibody [EPR19727-245] ab214938 IP in mouse heart lysate.
Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PDK4 antibody [EPR19727-245] ab214938 in mouse heart lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 0.5 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PDK4 antibody [EPR19727-245] ab214938).
All lanes: Immunoprecipitation - Anti-PDK4 antibody [EPR19727-245] (Anti-PDK4 antibody [EPR19727-245] ab214938)
Predicted band size: 46 kDa, 48 kDa
![Flow Cytometry (Intracellular) - Anti-PDK4 antibody [EPR19727-245] - BSA and Azide free (ab222401), expandable thumbnail](https://content.abcam.com/products/images/pdk4-antibody-epr19727-245-bsa-and-azide-free-ab222401--flow-cytometry-intracellular-img138687.jpg "Flow Cytometry (Intracellular) - Anti-PDK4 antibody [EPR19727-245] - BSA and Azide free (ab222401)")
Flow Cytometry (Intracellular) - Anti-PDK4 antibody [EPR19727-245] - BSA and Azide free (ab222401)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed C2C12 (Mouse myoblast cell line) cells labeling PDK4 with Anti-PDK4 antibody [EPR19727-245] ab214938 at 1/60 dilution (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PDK4 antibody [EPR19727-245] ab214938).
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