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Rabbit Recombinant Monoclonal PDLIM1/CLP36 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Mouse
Tested
Not recommended
Expected
Expected
Rat
Tested
Not recommended
Expected
Expected

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/500

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton (PubMed:10861853). Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts (By similarity).

Alternative names

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Rabbit Recombinant Monoclonal PDLIM1/CLP36 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR7186(B)

Purification technique

Affinity purification Protein A

Dissociation constant

9.54 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

PDLIM1 connects to actin filaments helping in maintaining cytoskeletal dynamics and cell structure. This protein is part of the larger complex involved in organizing actin filaments. PDLIM1 through interactions with cytoskeletal components regulates muscle contraction and cell motility. It stabilizes actin filaments through its PDZ domain and modulates signal transduction pathways linked to cellular adhesion and movement.

Activity summary

PDLIM1 also known as CLP36 is a protein that plays a mechanical role as an actin-binding protein. It is part of the PDZ-LIM family of proteins characterized by the presence of PDZ and LIM domains. PDLIM1 has a molecular mass of approximately 36 kDa and is involved in cytoskeletal organization by connecting actin filaments to the cross-linked network. It expresses mostly in tissues like the heart skeletal muscles and to some degree in others such as liver and lungs.

Pathways

Studies have identified PDLIM1's involvement in the regulation of signaling pathways such as the MAPK and Wnt pathways. Through these processes PDLIM1 interacts with proteins like kinases and phosphatases which play a role in signal transduction and cellular communication. By modulating these pathways PDLIM1 influences cell growth and differentiation processes critical in tissue development and maintenance.

Associated diseases and disorders

PDLIM1 is linked to conditions involving muscle malfunction and some cancer forms. Abnormal expression of PDLIM1 correlates with cardiomyopathies as it affects heart muscle function. Also changes in PDLIM1 levels have connections to several cancers possibly through pathways involving proteins such as β-catenin an important player in cell proliferation and tumor growth. Understanding these links may provide insights into novel therapeutic strategies for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015), expandable thumbnail

    Western blot - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015)

    All lanes: Western blot - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (AB129015) at 1/1000 dilution

    Lane 1: Human heart cell lysate at 10 µg

    Lane 2: L6 cell lysate at 10 µg

    Lane 3: 293T cell lysate at 10 µg

    Lane 4: Caco 2 cell lysate at 10 µg

    Lane 5: Saos 2 cell lysate at 10 µg

    Lane 6: C2C12 cell lysate at 10 µg

    Lane 7: Rat lung cell lysate at 10 µg

    Lane 8: Mouse lung cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 36 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015)

    Immunocytochemistry/ Immunofluorescence analysis of 293T (human embryonic kidney epithelial cell) cells labeling PDLIM1/CLP36 with purified ab129015 at 1/100 dilution (10 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL) was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015)

    ab129015, at 1/100 dilution, staining PDLIM1/CLP36 in permeabilized Saos 2 cells by intracellular flow cytometry or a Rabbit IgG negative control (green).

  • OI-RD Scanning - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015), expandable thumbnail

    OI-RD Scanning - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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