AB246868

Anti-PDLIM2 antibody

Name: Anti-PDLIM2 antibody (ab246868) | Abcam
Brand: Abcam Limited
SKU: AB246868

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Polyclonal PDLIM2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PDLIM2.

View Alternative Names

PP6345, PDLIM2, PDZ and LIM domain protein 2, PDZ-LIM protein mystique

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM2 antibody (AB246868)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM2 antibody (AB246868)

Paraffin-embedded human placenta tissue stained for PDLIM2 using ab246868 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-PDLIM2 antibody (AB246868)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PDLIM2 antibody (AB246868)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PDLIM2 (green) using ab246868at 4 μg/ml in ICC/IF.

Unknown

Western blot - Anti-PDLIM2 antibody (AB246868)

All lanes:

Western blot - Anti-PDLIM2 antibody (ab246868) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) whole cell lysate

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PDLIM2. The exact immunogen used to generate this antibody is proprietary information.

Q96JY6

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDLIM2 also known as Mystique or SLIM is a cytoskeletal protein that plays a role in linking actin filaments to various cellular structures. It contains a PDZ domain and a LIM domain which enable it to perform its function within the cell. PDLIM2 has a molecular mass of approximately 36 kDa. It is expressed in several tissue types including immune cells skeletal muscle and epithelial cells indicating its involvement in multiple cellular processes.

Biological function summary

PDLIM2 regulates the stability and activity of certain transcription factors and signaling molecules. It acts as an adapter protein to modulate the interaction of actin filaments with these factors. This protein also influences cell motility and adhesion acting as a critical component of a complex involving the actin cytoskeleton. Its regulation of signal transduction and transcription impacts cellular responses and maintenance of tissue integrity.

Pathways

PDLIM2 plays a central role in the NF-kB signaling pathway and the STAT pathway. By controlling the degradation and transport of nuclear factor NF-kB PDLIM2 affects immune responses and inflammation. Similarly it modulates STAT family proteins involved in pathways that trigger responses to cytokines and growth factors. PDLIM2 interacts with proteins such as RelA and STAT3 indicating its function in cell signaling and gene expression regulation.

PDLIM2 links to cancer and autoimmune disorders through its regulatory effects on inflammation and immune responses. Altered expression or function of PDLIM2 can contribute to the progression of certain cancers where it often interacts with oncogenic pathways. Additionally PDLIM2's involvement with proteins like NF-kB and STAT3 associates it with chronic inflammatory and autoimmune diseases further demonstrating its impact on pathological conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity.

See full target information PDLIM2

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 22:548 PubMed39238028

2024

A nanoscale visual exploration of the pathogenic effects of bacterial extracellular vesicles on host cells.

Applications

Unspecified application

Species

Unspecified reactive species

Minjae Kang,Min Jeong Kim,Dokyung Jeong,Hyung-Jun Lim,Ga-Eun Go,Uidon Jeong,Eunyoung Moon,Hee-Seok Kweon,Nae-Gyu Kang,Seung Jin Hwang,Sung Hun Youn,Bo Kyoung Hwang,Doory Kim

Cell biochemistry and function 41:542-552 PubMed37170668

2023

PDLIM2 can inactivate the TGF-β/Smad pathway to inhibit the malignant behavior of ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqin Lv,Hongrui Guo,Junxia Wang,Rui Ma,Lina Niu,Yun Shang

Neoplasma 69:1041-1053 PubMed35723199

2022

Exosomes from M2 macrophages promoted glycolysis in FaDu cells by inhibiting PDLIM2 expression to stabilize PFKL.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Wang,Guang-Yao Li,Lei Zhou,Huai-Li Jiang,Yue Yang,Hai-Tao Wu

Bioengineered 13:1779-1790 PubMed34713769

2021

Kruppel like factor 10 up-regulates PDZ and LIM domain containing protein 2 via nuclear factor kappa-B pathway to inhibit proliferation and inflammatory of fibroblastoid synovial cell in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Wang,Xuwen Zha,Shengting Ruan,Shoulin Yao,Xiaoyu Zhang

PLoS pathogens 15:e1007949 PubMed31374104

2019

HCV and flaviviruses hijack cellular mechanisms for nuclear STAT2 degradation: Up-regulation of PDLIM2 suppresses the innate immune response.

Applications

Unspecified application

Species

Unspecified reactive species

Michael A Joyce,Karyn M Berry-Wynne,Theodore Dos Santos,William R Addison,Nicola McFarlane,Tom Hobman,D Lorne Tyrrell

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDLIM2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM2 antibody (AB246868)

Immunocytochemistry/ Immunofluorescence - Anti-PDLIM2 antibody (AB246868)

Western blot - Anti-PDLIM2 antibody (AB246868)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise