AB224385

Anti-PDLIM5/ENH antibody

Name: Anti-PDLIM5/ENH antibody (ab224385) | Abcam
Brand: Abcam Limited
SKU: AB224385

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(1 Publication)

Rabbit Polyclonal PDLIM5/ENH antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PDLIM5 aa 100-300.

View Alternative Names

ENH, L9, PDLIM5, PDZ and LIM domain protein 5, Enigma homolog, Enigma-like PDZ and LIM domains protein

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM5/ENH antibody (AB224385)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM5/ENH antibody (AB224385)

Immunohistocehmcial analysis of paraffin-embedded human heart muscle staining PDLIM5/ENH with ab224385 at 1/50 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM5/ENH antibody (AB224385)

Immunohistochemical analysis of human testis tissue labeling PDLIM5/ENH with ab224385 at 1/50 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-PDLIM5/ENH antibody (AB224385)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PDLIM5/ENH antibody (AB224385)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for PDLIM5/ENH (green) using ab224385 at 4 μg/ml in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM5/ENH antibody (AB224385)

Paraffin-embedded human skeletal muscle tissue stained for PDLIM5/ENH using ab224385 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM5/ENH antibody (AB224385)

Immunohistochemical analysis of human prostate tissue labeling PDLIM5/ENH with ab224385 at 1/50 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM5/ENH antibody (AB224385)

Immunohistocehmcial analysis of paraffin-embedded human skeletal muscle staining PDLIM5/ENH with ab224385 at 1/50 dilution.

Unknown

Western blot - Anti-PDLIM5/ENH antibody (AB224385)

All lanes:

Western blot - Anti-PDLIM5/ENH antibody (ab224385) at 0.4 µg/mL

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Lane 3:

Plasma

Lane 4:

Liver tissue lysate

Lane 5:

Tonsil tissue lysate

Predicted band size: 64 kDa

false

Supplier Data

Western blot - Anti-PDLIM5/ENH antibody (AB224385)

All lanes:

Western blot - Anti-PDLIM5/ENH antibody (ab224385) at 1/50 dilution

All lanes:

A549 cell lysate

Predicted band size: 64 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PDLIM5 aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

Q96HC4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDLIM5/ENH also known as Enhancer of Lim Domain Protein 5 is a cytoskeletal protein with a molecular mass of approximately 62 kDa. This protein is primarily expressed in heart and skeletal muscle tissues. PDLIM5/ENH binds to actin filaments and is involved in organizing the cytoskeleton. It acts as an adapter or scaffold protein linking other proteins to the cytoskeleton and helping in maintaining structural integrity and function in muscle cells.

Biological function summary

PDLIM5/ENH plays a significant role in cardiac muscle development and function. It is also a part of the protein complex that interacts with other cytoskeletal proteins to stabilize actin filament structures. This interaction is important for muscle contraction and cellular signaling. PDLIM5/ENH is also involved in transducing mechanical signals into biochemical signals which affect cell differentiation and growth.

Pathways

PDLIM5/ENH integrates into important signaling pathways like the MAPK and Wnt pathways. In the MAPK pathway PDLIM5/ENH interacts with proteins involving cell growth and differentiation such as ERK. Through the Wnt pathway it plays a role in tissue development and regeneration by working alongside proteins like beta-catenin. These pathways highlight its contribution to essential cellular processes that affect muscle and cardiac function.

PDLIM5/ENH is associated with cardiac hypertrophy and dilated cardiomyopathy. Alterations in its function can impact cardiac muscle structure leading to pathological changes. Additionally its interaction with proteins like desmin in muscle tissues links it to muscle disorder pathologies. Understanding PDLIM5/ENH's role in these diseases may provide insights into therapeutic targets for cardiac and muscle-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play an important role in the heart development by scaffolding PKC to the Z-disk region. May play a role in the regulation of cardiomyocyte expansion. Isoforms lacking the LIM domains may negatively modulate the scaffolding activity of isoform 1. Overexpression promotes the development of heart hypertrophy. Contributes to the regulation of dendritic spine morphogenesis in neurons. May be required to restrain postsynaptic growth of excitatory synapses. Isoform 1, but not isoform 2, expression favors spine thinning and elongation.

See full target information PDLIM5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

European journal of medical research 29:257 PubMed38689322

2024

Overexpressed KCNK1 regulates potassium channels affecting molecular mechanisms and biological pathways in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Xiao-Song Chen,Ying Wei,Xiao-Min Wang,Xian-Jin Chen,Bang-Teng Chi,Lin-Qing Huang,Rong-Quan He,Zhi-Guang Huang,Qi Li,Gang Chen,Juan He,Mei Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDLIM5/ENH antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM5/ENH antibody (AB224385)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM5/ENH antibody (AB224385)

Immunocytochemistry/ Immunofluorescence - Anti-PDLIM5/ENH antibody (AB224385)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM5/ENH antibody (AB224385)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM5/ENH antibody (AB224385)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM5/ENH antibody (AB224385)

Western blot - Anti-PDLIM5/ENH antibody (AB224385)

Western blot - Anti-PDLIM5/ENH antibody (AB224385)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise