AB85967

Anti-PDLIM5/ENH antibody

Name: Anti-PDLIM5/ENH antibody (ab85967) | Abcam
Brand: Abcam Limited
SKU: AB85967
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal PDLIM5/ENH antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PDLIM5 aa 500-600.

View Alternative Names

ENH, L9, PDLIM5, PDZ and LIM domain protein 5, Enigma homolog, Enigma-like PDZ and LIM domains protein

2 Images

Unknown

Immunoprecipitation - Anti-PDLIM5/ENH antibody (AB85967)

1mg HeLa whole cell lysate was immunoprecipitated using ab85967 at 3μg. 20% of IP was loaded/lane and PDLIM5/ENH was detected with ab85967 at 1μg/ml (lane 1) or a control IgG (lane 2).
Detection : chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-PDLIM5/ENH antibody (ab85967)

Predicted band size: 64 kDa

false

Unknown

Western blot - Anti-PDLIM5/ENH antibody (AB85967)

All lanes:

Western blot - Anti-PDLIM5/ENH antibody (ab85967) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 64 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PDLIM5 aa 500-600. The exact immunogen used to generate this antibody is proprietary information.

Q96HC4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDLIM5/ENH also known as Enhancer of Lim Domain Protein 5 is a cytoskeletal protein with a molecular mass of approximately 62 kDa. This protein is primarily expressed in heart and skeletal muscle tissues. PDLIM5/ENH binds to actin filaments and is involved in organizing the cytoskeleton. It acts as an adapter or scaffold protein linking other proteins to the cytoskeleton and helping in maintaining structural integrity and function in muscle cells.

Biological function summary

PDLIM5/ENH plays a significant role in cardiac muscle development and function. It is also a part of the protein complex that interacts with other cytoskeletal proteins to stabilize actin filament structures. This interaction is important for muscle contraction and cellular signaling. PDLIM5/ENH is also involved in transducing mechanical signals into biochemical signals which affect cell differentiation and growth.

Pathways

PDLIM5/ENH integrates into important signaling pathways like the MAPK and Wnt pathways. In the MAPK pathway PDLIM5/ENH interacts with proteins involving cell growth and differentiation such as ERK. Through the Wnt pathway it plays a role in tissue development and regeneration by working alongside proteins like beta-catenin. These pathways highlight its contribution to essential cellular processes that affect muscle and cardiac function.

PDLIM5/ENH is associated with cardiac hypertrophy and dilated cardiomyopathy. Alterations in its function can impact cardiac muscle structure leading to pathological changes. Additionally its interaction with proteins like desmin in muscle tissues links it to muscle disorder pathologies. Understanding PDLIM5/ENH's role in these diseases may provide insights into therapeutic targets for cardiac and muscle-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

May play an important role in the heart development by scaffolding PKC to the Z-disk region. May play a role in the regulation of cardiomyocyte expansion. Isoforms lacking the LIM domains may negatively modulate the scaffolding activity of isoform 1. Overexpression promotes the development of heart hypertrophy. Contributes to the regulation of dendritic spine morphogenesis in neurons. May be required to restrain postsynaptic growth of excitatory synapses. Isoform 1, but not isoform 2, expression favors spine thinning and elongation.

See full target information PDLIM5

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 11: PubMed34069539

2021

Mechanical Sensing Element PDLIM5 Promotes Osteogenesis of Human Fibroblasts by Affecting the Activity of Microfilaments.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolan Huang,Rongmei Qu,Yan Peng,Yuchao Yang,Tingyu Fan,Bing Sun,Asmat Ullah Khan,Shutong Wu,Kuanhai Wei,Chujiang Xu,Jingxing Dai,Jun Ouyang,Shizhen Zhong

The Journal of biological chemistry 291:19364-73 PubMed27458019

2016

Presynaptic N-Methyl-d-aspartate (NMDA) Receptor Activity Is Increased Through Protein Kinase C in Paclitaxel-induced Neuropathic Pain.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Dun Xie,Shao-Rui Chen,Hong Chen,Wei-An Zeng,Hui-Lin Pan

View all publications

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Product promise

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Anti-PDLIM5/ENH antibody

Immunoprecipitation - Anti-PDLIM5/ENH antibody (AB85967)

Western blot - Anti-PDLIM5/ENH antibody (AB85967)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise