AB86065

Anti-PDLIM7 antibody

Name: Anti-PDLIM7 antibody (ab86065) | Abcam
Brand: Abcam Limited
SKU: AB86065

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(1 Publication)

Rabbit Polyclonal PDLIM7 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PDLIM7 aa 100-200.

View Alternative Names

ENIGMA, PDLIM7, PDZ and LIM domain protein 7, LIM mineralization protein, Protein enigma, LMP

2 Images

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Immunoprecipitation - Anti-PDLIM7 antibody (AB86065)

Detection of PDLIM7 by Western blot of Immunprecipitate.
ab86065, at 1 µg/ml, staining PDLIM7 in HeLa whole cell lysate immunoprecipitated using ab86065 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-PDLIM7 antibody (ab86065)

Predicted band size: 50 kDa

false

Unknown

Western blot - Anti-PDLIM7 antibody (AB86065)

All lanes:

Western blot - Anti-PDLIM7 antibody (ab86065) at 0.04 µg/mL

Lane 1:

Hela whole cell lysate at 50 µg

Lane 2:

Hela whole cell lysate at 15 µg

Lane 3:

Hela whole cell lysate at 5 µg

Predicted band size: 50 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PDLIM7 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q9NR12

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDLIM7 also known as Enigma or LMP-1 is a protein involved in several cellular processes. It has a molecular mass of approximately 54 kDa and contains PDZ and LIM domains which facilitate its function. PDLIM7 is broadly expressed in tissues including heart skeletal muscle and osteoblasts. The protein acts mechanically by organizing cytoskeletal components and bridging interactions between actin filaments and other cellular structures.

Biological function summary

PDLIM7 plays a role in assembling protein complexes that influence structural and signaling pathways. This protein participates in bone development and remodeling processes linking it to skeletal system maintenance and repair. Through its LIM domains PDLIM7 facilitates the interaction with various binding partners. It serves as a scaffold helping to maintain proper cellular architecture and function.

Pathways

PDLIM7 integrates into the TGF-beta signaling cascade an important regulatory pathway in cellular differentiation and proliferation. It interacts with proteins such as SMADs which transmit signals from the cell membrane to the nucleus modulating gene expression. PDLIM7 also associates with the MAPK pathway influencing cellular responses to extracellular stimuli and stress.

Aberrant expression or function of PDLIM7 has been linked to bone-related diseases such as osteosarcoma and osteoporosis. Changes in PDLIM7 expression may disrupt normal bone density and growth contributing to these conditions. PDLIM7's interaction with proteins like SMADs in pathological contexts highlights its role in disease progression especially in diseases linked to abnormal bone formation or degradation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May function as a scaffold on which the coordinated assembly of proteins can occur. May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Involved in both of the two fundamental mechanisms of bone formation, direct bone formation (e.g. embryonic flat bones mandible and cranium), and endochondral bone formation (e.g. embryonic long bone development). Plays a role during fracture repair. Involved in BMP6 signaling pathway (By similarity).

See full target information PDLIM7

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Proteomics. Clinical applications 10:51-7 PubMed26147903

2015

Proteomic analysis of integrin-associated complexes from mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jila N Ajeian,Edward R Horton,Pablo Astudillo,Adam Byron,Janet A Askari,Angélique Millon-Frémillon,David Knight,Susan J Kimber,Martin J Humphries,Jonathan D Humphries

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PDLIM7 antibody

Immunoprecipitation - Anti-PDLIM7 antibody (AB86065)

Western blot - Anti-PDLIM7 antibody (AB86065)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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