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AB250595

Anti-PDLIM7 antibody [EPR13171] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal PDLIM7 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ENIGMA, PDLIM7, PDZ and LIM domain protein 7, LIM mineralization protein, Protein enigma, LMP

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM7 antibody [EPR13171] - BSA and Azide free (AB250595)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM7 antibody [EPR13171] - BSA and Azide free (AB250595)

This data was developed using ab182153, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human infiltrating ductal breast carcinoma tissue labeling PDLIM7 with ab182153 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-PDLIM7 antibody [EPR13171] - BSA and Azide free (AB250595)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PDLIM7 antibody [EPR13171] - BSA and Azide free (AB250595)

This data was developed using ab182153, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling PDLIM7 with ab182153 at 1/60 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Western blot - Anti-PDLIM7 antibody [EPR13171] - BSA and Azide free (AB250595)
  • WB

Lab

Western blot - Anti-PDLIM7 antibody [EPR13171] - BSA and Azide free (AB250595)

This data was developed using ab182153, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times :

Lane 1 : 1 minute;

Lanes 2 - 4 : 3 minutes.

Lane 1:

Western blot - Anti-PDLIM7 antibody [EPR13171] (<a href='/en-us/products/primary-antibodies/pdlim7-antibody-epr13171-ab182153'>ab182153</a>) at 1/10000 dilution

Lanes 2 - 4:

Western blot - Anti-PDLIM7 antibody [EPR13171] (<a href='/en-us/products/primary-antibodies/pdlim7-antibody-epr13171-ab182153'>ab182153</a>) at 1/2000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

Jurkat (human acute T cell leukemia) whole cell lysate at 10 µg

Lane 3:

WI-38 (human lung) whole cell lysate at 10 µg

Lane 4:

SK-OV-3 (human adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG (H+L) peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13171

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen sequence for this antibody shares 86% homology with the mouse PDLIM7 protein however in our hands this antibody did not work in mouse and rat samples in western blot (no bands detected). In some human cell lines, in addition to the band of interest detected at 50 kDa, this product also bound to a protein at ~37-40 kDa in weight. For best results it may be preferable to use a harsh lysis protocol for preparation of cell or tissue lysates. Please see notes in the western blot image below.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab250595 is the carrier-free version of ab182153.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDLIM7 also known as Enigma or LMP-1 is a protein involved in several cellular processes. It has a molecular mass of approximately 54 kDa and contains PDZ and LIM domains which facilitate its function. PDLIM7 is broadly expressed in tissues including heart skeletal muscle and osteoblasts. The protein acts mechanically by organizing cytoskeletal components and bridging interactions between actin filaments and other cellular structures.
Biological function summary

PDLIM7 plays a role in assembling protein complexes that influence structural and signaling pathways. This protein participates in bone development and remodeling processes linking it to skeletal system maintenance and repair. Through its LIM domains PDLIM7 facilitates the interaction with various binding partners. It serves as a scaffold helping to maintain proper cellular architecture and function.

Pathways

PDLIM7 integrates into the TGF-beta signaling cascade an important regulatory pathway in cellular differentiation and proliferation. It interacts with proteins such as SMADs which transmit signals from the cell membrane to the nucleus modulating gene expression. PDLIM7 also associates with the MAPK pathway influencing cellular responses to extracellular stimuli and stress.

Aberrant expression or function of PDLIM7 has been linked to bone-related diseases such as osteosarcoma and osteoporosis. Changes in PDLIM7 expression may disrupt normal bone density and growth contributing to these conditions. PDLIM7's interaction with proteins like SMADs in pathological contexts highlights its role in disease progression especially in diseases linked to abnormal bone formation or degradation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as a scaffold on which the coordinated assembly of proteins can occur. May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Involved in both of the two fundamental mechanisms of bone formation, direct bone formation (e.g. embryonic flat bones mandible and cranium), and endochondral bone formation (e.g. embryonic long bone development). Plays a role during fracture repair. Involved in BMP6 signaling pathway (By similarity).
See full target information PDLIM7
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:458 PubMed37736555

2023

S100A6 is a potential diagnostic and prognostic biomarker for human glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hong,Hui Zhang,Yufei Xiao,Lingwei Shen,Yun Qian
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com