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AB233517

Anti-PDP2 antibody

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(2 Publications)

Rabbit Polyclonal Pdp2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Pdp2 aa 1-200.
6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDP2 antibody (AB233517)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDP2 antibody (AB233517)

Formalin-fixed, paraffin-embedded rat cerebrum tissue stained for PDP2 using ab233517 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDP2 antibody (AB233517)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDP2 antibody (AB233517)

Formalin-fixed, paraffin-embedded rat stomach tissue stained for PDP2 using ab233517 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDP2 antibody (AB233517)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDP2 antibody (AB233517)

Formalin-fixed, paraffin-embedded rat liver tissue stained for PDP2 using ab233517 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-PDP2 antibody (AB233517)
  • WB

Supplier Data

Western blot - Anti-PDP2 antibody (AB233517)

All lanes:

Western blot - Anti-PDP2 antibody (ab233517) at 1 µg/mL

All lanes:

Mouse skeletal muscle lysate

Predicted band size: 60 kDa

false

Western blot - Anti-PDP2 antibody (AB233517)
  • WB

Supplier Data

Western blot - Anti-PDP2 antibody (AB233517)

All lanes:

Western blot - Anti-PDP2 antibody (ab233517) at 1 µg/mL

All lanes:

Recombinant rat PDP2 protein

Predicted band size: 60 kDa

false

Western blot - Anti-PDP2 antibody (AB233517)
  • WB

Supplier Data

Western blot - Anti-PDP2 antibody (AB233517)

All lanes:

Western blot - Anti-PDP2 antibody (ab233517) at 1 µg/mL

All lanes:

Rat serum

Predicted band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Rat Pdp2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

O88484

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDP2 also known as pyruvate dehydrogenase phosphatase catalytic subunit 2 plays a critical role in energy metabolism. The protein dephosphorylates the pyruvate dehydrogenase (PDH) complex activating it to facilitate the conversion of pyruvate to acetyl-CoA an important step in cellular respiration. PDP2 has a molecular mass of approximately 61 kDa and is expressed in various tissues with higher expression noted in skeletal muscle and heart tissues. Through its enzymatic action PDP2 regulates the activity of the PDH complex and therefore influences the energy balance of cells.
Biological function summary

PDP2 contributes to energy homeostasis by modulating the activity of the PDH complex a major component in the mitochondrial matrix. It ensures the optimal function of the PDH complex which itself is a large multi-subunit structure comprising several enzymes including E1 E2 and E3 subunits. PDP2's dephosphorylation activity is central in facilitating the switch between glycolysis and oxidative phosphorylation allowing cells to adapt their energy production according to metabolic demands. This protein therefore plays a valuable role in the cellular metabolic network impacting ATP production efficiency.

Pathways

PDP2 is significant in the regulation of the tricarboxylic acid (TCA) cycle and glycolysis. It is inherently involved in the TCA cycle as it modulates the flux of acetyl-CoA into the cycle by regulating PDH complex activity. PDP2 also interacts with other proteins involved in the energy metabolism pathway such as PDH kinase isoenzymes which perform an antagonistic function by inactivating the PDH complex through phosphorylation. This regulatory balance ensures that cellular energy demands are met accurately and efficiently.

Aberrations in PDP2 function have connections to metabolic disorders such as type 2 diabetes and heart failure. In type 2 diabetes impaired PDP2 activity can lead to reduced PDH complex activity contributing to altered glucose and lipid metabolism. Furthermore in heart failure inadequate PDP2 activity can disrupt energy production in cardiomyocytes exacerbating heart muscle dysfunction. Through these diseases PDP2 is linked with other metabolic proteins including insulin receptor substrates and mitochondrial dysfunction proteins illustrating its significance in both normal physiology and disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Pdp2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular metabolism 45:101154 PubMed33359401

2020

Insulin resistance is mechanistically linked to hepatic mitochondrial remodeling in non-alcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chris E Shannon,Mukundan Ragavan,Juan Pablo Palavicini,Marcel Fourcaudot,Terry M Bakewell,Ivan A Valdez,Iriscilla Ayala,Eunsook S Jin,Muniswamy Madesh,Xianlin Han,Matthew E Merritt,Luke Norton

Cell reports 33:108264 PubMed33053349

2020

Activation of the CARD8 Inflammasome Requires a Disordered Region.

Applications

Unspecified application

Species

Unspecified reactive species

Ashley J Chui,Andrew R Griswold,Cornelius Y Taabazuing,Elizabeth L Orth,Kuo Gai,Sahana D Rao,Daniel P Ball,Jeffrey C Hsiao,Daniel A Bachovchin
View all publications
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Product promise

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