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Rabbit Recombinant Monoclonal PDPK1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat, Pig samples. Cited in 27 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 antibody [EP569Y] (AB52893), expandable thumbnail
  • Western blot - Anti-PDPK1 antibody [EP569Y] (AB52893), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 antibody [EP569Y] (AB52893), expandable thumbnail
  • Western blot - Anti-PDPK1 antibody [EP569Y] (AB52893), expandable thumbnail
  • Western blot - Anti-PDPK1 antibody [EP569Y] (AB52893), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Not recommended
Not recommended
Tested
Not recommended
Mouse
Expected
Not recommended
Not recommended
Tested
Not recommended
Rat
Expected
Not recommended
Not recommended
Tested
Not recommended
Pig
Expected
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1/150

Notes

The mouse, rat and pig recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and pig.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat, Pig

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Pig, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Pig, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Pig

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Pig, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages. Isoform 3 is catalytically inactive.

Alternative names

3-phosphoinositide-dependent protein kinase 1, hPDK1, PDPK1, PDK1

Recommended products

Rabbit Recombinant Monoclonal PDPK1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat, Pig samples. Cited in 27 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP569Y

Purification technique

Affinity purification Protein A

Specificity

This antibody detects PDK1 both when phosphorylated and non-phosphorylated on Tyrosine 373 and 376.
The mouse, rat and pig recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and pig.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PDPK1 also known as 3-phosphoinositide-dependent protein kinase-1 or PDK1 is a protein kinase with a molecular mass of approximately 63 kDa. It plays an important mechanical role in the activation of other kinases through phosphorylation on threonine or serine residues. PDPK1 is widely expressed in various tissues including heart lung pancreas and liver reflecting its significant roles in cellular signalling.

Biological function summary

PDPK1 is a master regulator of several AGC kinase family enzymes. It functions as a docking station coordinating with other proteins for activation processes. For example PDPK1 helps in activating PKB/Akt which is required for cell survival and growth. PDPK1 forms part of signaling complexes that manage processes like metabolism growth and survival.

Pathways

PDPK1 plays an essential role in the PI3K/AKT pathway which is an important signaling pathway for cellular growth and survival. This pathway begins with the activation of PI3K leading to the production of PIP3 which creates a binding site for PDPK1 at the plasma membrane where it activates PKB/Akt. Also PDPK1 interacts with NF-κB pathway components linking it to processes in immune response regulation and inflammation.

Associated diseases and disorders

PDPK1 has associations with cancer and Type 2 diabetes. Abnormal activity of PDPK1 is observed in several cancer types indicating its role in uncontrolled cell growth. In Type 2 diabetes PDPK1 influences insulin signaling disruptions and glucose metabolism. PDPK1 connects these conditions through interactions with proteins like mTOR which influences cell growth and metabolism further exemplifying its importance in disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 antibody [EP569Y] (ab52893), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 antibody [EP569Y] (ab52893)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling PDPK1 with Purified ab52893 at 1:150 dilution(2.4 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893), expandable thumbnail

    Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)

    The molecular weight observed is consistent with what has been described in PMID: 30171166

    All lanes: Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893) at 1/1000 dilution

    All lanes: F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/5000 dilution

    Predicted band size: 63 kDa

    Observed band size: 52-65 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 antibody [EP569Y] (ab52893), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 antibody [EP569Y] (ab52893)

    ab52893 (1:100) staining human PDPK1 in human prostate carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893), expandable thumbnail

    Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)

    The molecular weight observed is consistent with what has been described in PMID: 30171166

    All lanes: Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 2: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/5000 dilution

    Predicted band size: 63 kDa

    Observed band size: 52-65 kDa

  • Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893), expandable thumbnail

    Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)

    The molecular weight observed is consistent with what has been described in PMID: 30171166

    All lanes: Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893) at 1/1000 dilution

    Lane 1: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 20 µg

    Lane 2: Rat testis lysates at 20 µg

    Lane 3: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg

    Lane 4: 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 5: PIEC (PIG iliac artery endothelial cell) whole cell lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 63 kDa

    Observed band size: 52-65 kDa

  • Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893), expandable thumbnail

    Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: PDPK1 knockout HAP1 cell lysate (20 μg)
    Lane 3: HEK293 cell lysate (20 μg)
    Lane 4: MCF7 cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab52893 observed at 70 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab52893 was shown to recognize PDPK1 when PDPK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and PDPK1 knockout samples were subjected to SDS-PAGE. ab52893 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)

    Predicted band size: 63 kDa

  • Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893), expandable thumbnail

    Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)

    All lanes: Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893) at 1/1000 dilution

    All lanes: 293 cell lysate

    Predicted band size: 63 kDa

    Observed band size: 63 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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