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AB206341

Anti-PDPK1 (phospho S396) antibody [EPR19819]

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(1 Publication)

Rabbit Recombinant Monoclonal PDPK1 phospho S396 antibody. Suitable for IP, Dot, WB and reacts with Human, Synthetic peptide - Human samples. Cited in 1 publication.

View Alternative Names

PDK1, PDPK1, 3-phosphoinositide-dependent protein kinase 1, hPDK1

4 Images
Immunoprecipitation - Anti-PDPK1 (phospho S396) antibody [EPR19819] (AB206341)
  • IP

Supplier Data

Immunoprecipitation - Anti-PDPK1 (phospho S396) antibody [EPR19819] (AB206341)

PDPK1 (phospho S396) was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate, transfected with PDPK1, with ab206341 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab206341 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HEK-293T transfected with PDPK1 whole cell lysate, 10 μg (Input).

Lane 2 : ab206341 IP in HEK-293T transfected with PDPK1 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab206341 in HEK-293T transfected with PDPK1 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

The plasmids were kindly provided by our collaborator Dr. Yi Rao, Peking University.

All lanes:

Immunoprecipitation - Anti-PDPK1 (phospho S396) antibody [EPR19819] (ab206341)

Predicted band size: 63 kDa

Observed band size: 63 kDa

false

Western blot - Anti-PDPK1 (phospho S396) antibody [EPR19819] (AB206341)
  • WB

Unknown

Western blot - Anti-PDPK1 (phospho S396) antibody [EPR19819] (AB206341)

Blocking/Dilution buffer : 5% BSA/TBST.

All lanes:

Western blot - Anti-PDPK1 (phospho S396) antibody [EPR19819] (ab206341) at 1/1000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate transfected with PDPK1 WT at 10 µg

Lane 2:

HEK-293T whole cell lysate transfected with PDPK1 (WT), then treated with alkaline phosphatase for 1 hour at 10 µg

Lane 3:

HEK-293T whole cell lysate transfected with PDPK1 S396A mutant at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution

Predicted band size: 63 kDa

Observed band size: 60 kDa,63 kDa

false

Exposure time: 5s

This data was kindly provided by Dr. Yi Rao, Peking University.

Western blot - Anti-PDPK1 (phospho S396) antibody [EPR19819] (AB206341)
  • WB

Supplier Data

Western blot - Anti-PDPK1 (phospho S396) antibody [EPR19819] (AB206341)

5% NFDM/TBST : Blocking and dilution buffer.

The plasmids were kindly provided by our collaborator Dr. Yi Rao, Peking University.

Based on sequence alignment, this antigen can recognize 5 isoforms, the predicted MW are 63kDa, 58 kDa, 60 kDa, 48 kDa and 51kDa, respectively.

All lanes:

Western blot - Anti-PDPK1 (phospho S396) antibody [EPR19819] (ab206341) at 1/5000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate transfected with PDPK1 S396A mutant at 10 µg

Lane 2:

HEK-293T whole cell lysate transfected with PDPK1 (WT) at 10 µg

Lane 3:

HEK-293T whole cell lysate transfected with PDPK1 (WT), then treated with alkaline phosphatase for 1 hour at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 63 kDa

Observed band size: 60 kDa,63 kDa

false

Exposure time: 3min

Dot Blot - Anti-PDPK1 (phospho S396) antibody [EPR19819] (AB206341)
  • Dot

Supplier Data

Dot Blot - Anti-PDPK1 (phospho S396) antibody [EPR19819] (AB206341)

Dot blot analysis of PDPK1 (phospho S396) labeled with ab206341 at 1/1000 dilution.

Lane 1 : PDPK1 (phospho S393) peptide (aa391-401)

Lane 2 : PDPK1 (phospho S393) peptide (aa386-396)

Lane 3 : PDPK1 non-phospho peptide (aa392-402)

Lane 4 : PDPK1 (phospho S396) peptide (aa392-401)

Lane 5 : PDPK1 (phospho S396) peptide(aa393-402)

Goat Anti-Rabbit IgG (H+L)Peroxidase conjugated (ab97051) at 1/20000 dilution was used as secondary antibody.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

  • Carrier free

    Anti-PDPK1 (phospho S396) antibody [EPR19819] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19819

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Synthetic peptide - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDPK1 also known as 3-phosphoinositide-dependent protein kinase-1 or PDK1 is a protein kinase with a molecular mass of approximately 63 kDa. It plays an important mechanical role in the activation of other kinases through phosphorylation on threonine or serine residues. PDPK1 is widely expressed in various tissues including heart lung pancreas and liver reflecting its significant roles in cellular signalling.
Biological function summary

PDPK1 is a master regulator of several AGC kinase family enzymes. It functions as a docking station coordinating with other proteins for activation processes. For example PDPK1 helps in activating PKB/Akt which is required for cell survival and growth. PDPK1 forms part of signaling complexes that manage processes like metabolism growth and survival.

Pathways

PDPK1 plays an essential role in the PI3K/AKT pathway which is an important signaling pathway for cellular growth and survival. This pathway begins with the activation of PI3K leading to the production of PIP3 which creates a binding site for PDPK1 at the plasma membrane where it activates PKB/Akt. Also PDPK1 interacts with NF-κB pathway components linking it to processes in immune response regulation and inflammation.

PDPK1 has associations with cancer and Type 2 diabetes. Abnormal activity of PDPK1 is observed in several cancer types indicating its role in uncontrolled cell growth. In Type 2 diabetes PDPK1 influences insulin signaling disruptions and glucose metabolism. PDPK1 connects these conditions through interactions with proteins like mTOR which influences cell growth and metabolism further exemplifying its importance in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases (PubMed : 10226025, PubMed : 10480933, PubMed : 10995762, PubMed : 12167717, PubMed : 14585963, PubMed : 14604990, PubMed : 16207722, PubMed : 16251192, PubMed : 17327236, PubMed : 17371830, PubMed : 18835241, PubMed : 9094314, PubMed : 9368760, PubMed : 9445476, PubMed : 9445477, PubMed : 9707564, PubMed : 9768361). Its targets include : protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), TSSK3, protein kinase PKN (PKN1 and PKN2) (PubMed : 10226025, PubMed : 10480933, PubMed : 10995762, PubMed : 12167717, PubMed : 14585963, PubMed : 14604990, PubMed : 16207722, PubMed : 16251192, PubMed : 17327236, PubMed : 17371830, PubMed : 18835241, PubMed : 9094314, PubMed : 9368760, PubMed : 9445476, PubMed : 9707564, PubMed : 9768361). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage (PubMed : 10226025, PubMed : 12167717, PubMed : 9094314). Negatively regulates the TGF-beta-induced signaling by : modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta (PubMed : 17327236). Activates PPARG transcriptional activity and promotes adipocyte differentiation (By similarity). Activates the NF-kappa-B pathway via phosphorylation of IKKB (PubMed : 16207722). The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II (PubMed : 14585963). Controls proliferation, survival, and growth of developing pancreatic cells (By similarity). Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells (By similarity). Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis (PubMed : 17371830). Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response (By similarity). Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses (By similarity). Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages (By similarity).. Isoform 3. Catalytically inactive.
See full target information PDPK1 phospho S396
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncogenesis 6:e323 PubMed28436990

2017

TCRP1 promotes NIH/3T3 cell transformation by over-activating PDK1 and AKT1.

Applications

Unspecified application

Species

Unspecified reactive species

C Wang,H Liu,Q Qiu,Z Zhang,Y Gu,Z He
View all publications
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