Rabbit Recombinant Monoclonal PDRG1 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-PDRG1 antibody [EPR6062(2)] - BSA and Azide free (AB248379), expandable thumbnail](https://content.abcam.com/products/images/pdrg1-antibody-epr60622-bsa-and-azide-free-ab248379--western-blot-img104254.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PDRG1 antibody [EPR6062(2)] - BSA and Azide free (AB248379)")
![OI-RD Scanning - Anti-PDRG1 antibody [EPR6062(2)] - BSA and Azide free (AB248379), expandable thumbnail](https://content.abcam.com/products/images/pdrg1-antibody-epr60622-bsa-and-azide-free-ab248379--oi-rd-scanning-img83618.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-PDRG1 antibody [EPR6062(2)] - BSA and Azide free (AB248379)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Predicted | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Predicted | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
May play a role in chaperone-mediated protein folding.
Alternative names
C20orf126, PDRG, PDRG1, p53 and DNA damage-regulated protein 1
Recommended products
Rabbit Recombinant Monoclonal PDRG1 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6062(2)
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 2.25 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248379 is the carrier-free version of Anti-PDRG1 antibody [EPR6062(2)] ab131219.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDRG1 also known as p53 and DNA damage-regulated gene 1 is a protein with a mass of approximately 31 kilodaltons. It functions mechanically by influencing cell survival and proliferation processes. PDRG1 has a role in cellular response to stress with its expression prominently observed in testis tissues. Researchers have noted this protein's regulation under stress conditions indicating its involvement in maintaining cellular homeostasis.
- Biological function summary
PDRG1 interacts with several proteins to carry out its functions potentially forming part of protein complexes influencing DNA repair mechanisms. It participates in cellular protection against genotoxic stress ensuring genome integrity. The protein's presence in testis suggests a role in germ cell development and the maintenance of reproductive health. This emphasizes its importance in the cellular systems governing DNA maintenance.
- Pathways
PDRG1 plays a role in DNA damage response pathways specifically in the p53 signaling network. It interfaces with proteins such as p53 and those involved in apoptosis linking it to cellular death and survival pathways during stress. PDRG1's interaction with p53 suggests its close involvement in pathways that control cell cycle arrest and repair following DNA damage therefore participating in decision-making processes of cellular fate.
- Associated diseases and disorders
PDRG1 links to cancer development and progression through its modulation of the p53 network. Altered expression of PDRG1 associates with tumorigenesis particularly in testicular cancer where its dysregulation may disrupt normal cell cycle control. In relation to Alzheimer’s disease studies have suggested a potential involvement of PDRG1 with proteins like tau impacting neurodegenerative processes. Therefore PDRG1 plays a significant role in cellular dysfunctions seen in cancer and neurodegeneration.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-PDRG1 antibody [EPR6062(2)] - BSA and Azide free (ab248379), expandable thumbnail](https://content.abcam.com/products/images/pdrg1-antibody-epr60622-bsa-and-azide-free-ab248379--western-blot-img104254.jpg "Western blot - Anti-PDRG1 antibody [EPR6062(2)] - BSA and Azide free (ab248379)")
Western blot - Anti-PDRG1 antibody [EPR6062(2)] - BSA and Azide free (ab248379)
This data was developed using Anti-PDRG1 antibody [EPR6062(2)] ab131219, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-PDRG1 antibody [EPR6062(2)] (Anti-PDRG1 antibody [EPR6062(2)] ab131219) at 1/1000 dilution
Lane 1: 293T cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: Jurkat cell lysate at 10 µg
Lane 4: MCF-7 cell lysate at 10 µg
Predicted band size: 15 kDa
![OI-RD Scanning - Anti-PDRG1 antibody [EPR6062(2)] - BSA and Azide free (ab248379), expandable thumbnail](https://content.abcam.com/products/images/pdrg1-antibody-epr60622-bsa-and-azide-free-ab248379--oi-rd-scanning-img83618.jpg "OI-RD Scanning - Anti-PDRG1 antibody [EPR6062(2)] - BSA and Azide free (ab248379)")
OI-RD Scanning - Anti-PDRG1 antibody [EPR6062(2)] - BSA and Azide free (ab248379)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Downloads
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- Visit the general protocols
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