AB251797

Anti-PDSS2 antibody

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(2 Publications)

Rabbit Polyclonal PDSS2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PDSS2 aa 300 to C-terminus.

View Alternative Names

C6orf210, DLP1, PDSS2, All trans-polyprenyl-diphosphate synthase PDSS2, All-trans-decaprenyl-diphosphate synthase subunit 2, Candidate tumor suppressor protein, Decaprenyl pyrophosphate synthase subunit 2, Decaprenyl-diphosphate synthase subunit 2, Solanesyl-diphosphate synthase subunit 2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDSS2 antibody (AB251797)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDSS2 antibody (AB251797)

Paraffin-embedded human colon tissue stained for PDSS2 using ab251797 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-PDSS2 antibody (AB251797)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PDSS2 antibody (AB251797)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for PDSS2 (green) using ab251797 at 4 µg/ml in ICC/IF.

Unknown

Western blot - Anti-PDSS2 antibody (AB251797)

All lanes:

Western blot - Anti-PDSS2 antibody (ab251797) at 0.4 µg/mL

All lanes:

RT4 (human urinary bladder cancer cell line) cell lysate

Predicted band size: 44 kDa

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Western blot - Anti-PDSS2 antibody (AB251797)

All lanes:

Western blot - Anti-PDSS2 antibody (ab251797) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 44 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PDSS2 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q86YH6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDSS2 also known as decaprenyl-diphosphate synthase subunit 2 is an enzyme that plays an important role in the biosynthesis of coenzyme Q (CoQ). This enzyme has a mass of approximately 43 kDa. PDSS2 is primarily expressed in the mitochondria where it participates in the process of extending the prenyl side chain of ubiquinone. Its activity is essential for the creation of isoprenoid compounds and it is involved in the synthesis of decaprenyl pyrophosphate which is an important precursor in the CoQ biosynthesis pathway.

Biological function summary

The enzyme extends the polyprenyl side chain of ubiquinone which is essential for its function in the mitochondrial electron transport chain. PDSS2 works closely with other enzymes in a multiprotein complex to ensure appropriate CoQ length and function. This activity is important for electron transport and ATP production. The enzymatic function of PDSS2 maintains mitochondrial integrity and performance by supporting oxidative phosphorylation.

Pathways

PDSS2 is involved in the ubiquinone and other terpenoid-quinone biosynthesis pathways. In these pathways PDSS2 interacts with proteins such as COQ2 which also contributes to the biosynthesis of CoQ. Together they ensure the proper synthesis of CoQ10 which is important for the mitochondrial respiratory chain pathway. This connection between PDSS2 and COQ2 highlights their collaboration in producing an important component necessary for cellular energy metabolism.

Mutations in PDSS2 have been linked to Coenzyme Q10 deficiency which can lead to mitochondrial dysfunction. This deficiency affects organs with high energy demands such as muscles and the nervous system. Additionally dysregulation of the CoQ biosynthesis pathway due to PDSS2 mutations is associated with various disorders including kidney dysfunction. PDSS2 is also related to other proteins like COQ9 which influence CoQ levels and their effect on cellular health and disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Heterotetrameric enzyme that catalyzes the condensation of farnesyl diphosphate (FPP), which acts as a primer, and isopentenyl diphosphate (IPP) to produce prenyl diphosphates of varying chain lengths and participates in the determination of the side chain of ubiquinone (PubMed : 16262699). Supplies nona and decaprenyl diphosphate, the precursors for the side chain of the isoprenoid quinones ubiquinone-9 (Q9) and ubiquinone-10 (Q10) respectively (PubMed : 16262699). The enzyme adds isopentenyl diphosphate molecules sequentially to farnesyl diphosphate with trans stereochemistry (PubMed : 16262699). May play a role during cerebellar development (By similarity). May regulate mitochondrial respiratory chain function (By similarity).

See full target information PDSS2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:19602 PubMed37950047

2023

Identification of DNA damage response-related genes as biomarkers for castration-resistant prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Masashi Oshima,Ken-Ichi Takayama,Yuta Yamada,Naoki Kimura,Haruki Kume,Tetsuya Fujimura,Satoshi Inoue

Journal of cardiovascular pharmacology 77:767-776 PubMed33929387

2021

PDSS2 Inhibits the Ferroptosis of Vascular Endothelial Cells in Atherosclerosis by Activating Nrf2.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Yang,Hejian Song,Delu Yin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PDSS2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDSS2 antibody (AB251797)

Immunocytochemistry/ Immunofluorescence - Anti-PDSS2 antibody (AB251797)

Western blot - Anti-PDSS2 antibody (AB251797)

Western blot - Anti-PDSS2 antibody (AB251797)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise