AB227586

Anti-PDX1 antibody

Name: Anti-PDX1 antibody (ab227586) | Abcam
Brand: Abcam Limited
SKU: AB227586
Price: 510 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal PDX1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PDX1.

View Alternative Names

IPF1, STF1, PDX1, Pancreas/duodenum homeobox protein 1, PDX-1, Glucose-sensitive factor, Insulin promoter factor 1, Insulin upstream factor 1, Islet/duodenum homeobox-1, Somatostatin-transactivating factor 1, GSF, IPF-1, IUF-1, IDX-1, STF-1

1 Images

Supplier Data

Western blot - Anti-PDX1 antibody (AB227586)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-PDX1 antibody (ab227586) at 1/1000 dilution

Lane 1:

Mouse pancreas tissue extracts at 50 µg

Lane 2:

Mouse liver tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 31 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PDX1. The exact immunogen used to generate this antibody is proprietary information.

P52945

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pancreatic duodenal homeobox 1 (PDX1) also known as insulin promoter factor 1 (IPF1) is an important transcription factor with a molecular weight of approximately 31 kDa. PDX1 plays an essential role in the development and function of the pancreas. This protein is mainly expressed in the pancreatic beta cells where it regulates gene expression linked to insulin production. Its expression also extends to the duodenum where it influences other developmental processes.

Biological function summary

PDX1 plays a critical role in pancreatic development beta-cell differentiation and insulin gene transcription. It functions as part of a protein complex that controls key aspects of beta-cell identity and function. The interactions within this complex allow PDX1 to effectively modulate pancreatic gene expression and maintain glucose homeostasis.

Pathways

PDX1 is deeply involved in the insulin secretion pathway and the regulation of glucose metabolism. It interacts with other transcription factors such as MAFA and NEUROD1 to carry out its role in these pathways. The presence of PDX1 in these pathways ensures the proper regulation of genes essential for insulin production and release connecting it to various metabolic processes.

PDX1 associates mainly with diabetes mellitus and pancreatic agenesis. Mutations or functional impairments in PDX1 affect insulin production leading to diabetes. Furthermore in pancreatic agenesis where the pancreas fails to develop PDX1 malfunctions are often implicated. PDX1 interactions with proteins like MAFA and NEUROD1 during this process are critical; alterations in these relationships can exacerbate disease states linking PDX1 to both genetic and acquired forms of pancreatic dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1 : PBX1b : MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.

See full target information PDX1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Animals : an open access journal from MDPI 15: PubMed40941427

2025

Histopathological and Immunohistochemical Study of Neoplastic Cell Heterogeneity in Early and Advanced Ovine Pulmonary Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Raúl A Reséndiz-Pozos,Jose María González-Saínz,Aurora Ortín,Javier Asin,María Climent,Luis Borderías,Marcelo De Las Heras

Cell transplantation 31:9636897221081483 PubMed35236160

2022

The Roles of Different Multigene Combinations of , , , , and in the Reprogramming of Canine ADSCs Into IPCs.

Applications

Unspecified application

Species

Unspecified reactive species

Dengke Gao,Pengxiu Dai,Zhixin Fan,Jinglu Wang,Yihua Zhang

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