Anti-PDX1 antibody

• IHC-P

AbReview12460****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody (AB47267)

ab47267 staining PDX1 in rat pancreatic tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C. The primary antibody was diluted 1/500 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

This image is courtesy of Antibody Solutions Ltd.

• IHC-P

Project

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody (AB47267)

ab47267 staining PDX1 in human appendix tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C (5 minutes for peroxide block and 10 minutes for protein block). The primary antibody was diluted 1/500 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as the secondary.

This image is courtesy of Antibody Solutions Ltd.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody (AB47267)

Immunostaining of islet-epithelial clusters from human foetal pancreata with ab47267 (green). Nuclei are visualised with DAPI (blue).

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody (AB47267)

Immunohistochemical analysis of murine pancreatic islets, staining PDX1 (red) with ab47267.

Tissue was fixed in formalin, permeablized with 0.25% Triton X-100, and blocked in blocking solution for 1 hour at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody overnight at 4°C and a fluorescent-conjugated anti-rabbit IgG was used as the secondary antibody.

Image from Zhang Y et al., PLoS One. 2012;7(5):e36675. Epub 2012 May 7. Fig 7.; doi:10.1371/journal.pone.0036675; May 7, 2012, PLoS ONE 7(5): e36675.