Anti-PDX1 antibody

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody (AB47308)

PDX1 staining of Rin-5F cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with Triton X-100 for 5 minutes and then blocked with 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab47308, Guinea pig polyclonal to PDX1 at 1µg/ml. Cells were then incubated with ab150185 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) at 2µg/ml (shown in pseudocolour Green). Nuclear DNA was labelled in blue with DAPI. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Images were acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody (AB47308)

Immunostaining of islets from 4 week-old mice with ab47308 guinea pig anti-PDX1 (red) and an anti-insulin antibody (green).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody (AB47308)

PDX1 staining of Rin-5F cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with Triton X-100 for 5 minutes and then blocked with 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab47308, Guinea pig polyclonal to PDX1 at 1 µg/ml. Cells were then incubated with ab150188 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 594) at 2 µg/ml (shown in pseudocolour Magenta). Nuclear DNA was labelled in blue with DAPI. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Images were acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody (AB47308)

PDX1 staining of Rin-5F cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with Triton X-100 for 5 minutes and then blocked with 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab47308, Guinea pig polyclonal to PDX1 at 1µg/ml. Cells were then incubated with ab175714 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 568) at 2µg/ml (shown in pseudocolour Magenta). Nuclear DNA was labelled in blue with DAPI. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Images were acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody (AB47308)

PDX1 staining of Rin-5F cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with Triton X-100 for 5 minutes and then blocked with 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab47308, Guinea pig polyclonal to PDX1 at 1µg/ml. Cells were then incubated with ab150186 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 555) at 2µg/ml (shown in pseudocolour Magenta). Nuclear DNA was labelled in blue with DAPI. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Images were acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody (AB47308)

PDX1 staining of Rin-5F cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with Triton X-100 for 5 minutes and then blocked with 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab47308, Guinea pig polyclonal to PDX1 at 1µg/ml. Cells were then incubated with ab150187 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647) at 2µg/ml (shown in pseudocolour Magenta). Nuclear DNA was labelled in blue with DAPI. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Images were acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.